簡(jiǎn)介：華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文核盤(pán)菌弱毒相關(guān)病毒基因組分子生物學(xué)特性的初步研究姓名衛(wèi)冬妹申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師姜道宏20030501華中農(nóng)業(yè)大學(xué)2003年碩士論文核盤(pán)苗弱毒相關(guān)病毒基因組分子生物學(xué)特性的初步研究PRELIMINARYSTUDIESONTHE64KBDSRNAGENOMEOFTHEMYCOVIRUSINFECTINGTHEHYPOVIRULENTISOLATEEP1PNOFSCLEROTINIASCLEROTIORUMABSTRAETINTHISDISSERTATIONTHE64KBDSR_NAGENOMEOFTHEMYCOVIMSINFECTINGTHEHYPOVIRULENTISOLATEEP1PNOFSCLEMTINIASCLEROTTORURASSHVWASSTUDIEDUSINGTECHNIQUESOFEDNASYNTHESIS，CLONING，ANDSEQUENCINGTHERELATIONSHIPBETWEENHYPOVIRULENCEOFSCLEROTINIASCLEROTIORUMEP1PNANDTHEDSR2QAMYCOVIRUSWASFURTHERCONFIRMEDATTILEMOLECULARLEVELRESETSACHIEYEDSOFARINCLUDELPOSITIVEEDNACLONESOFTHE64KBDS_RNAOFEP1PNWEREOBTAINEDAFTERSEQUENCINGTHEINSERTIALCDNAFRAGMENTS，INTHEPLASMIDPARTIALEDNASEQUENCEOFTHE64KBRNAGENOMEOF4629NUCTEOTIDESWASALIGNEDWITHDNAMANPROGRAMTHESEQUENCE4629BPWASSUBMITTEDTOTHEGENBANKWITHTHEACCESSIONNUMBERAYL472602AINCOMPLETEOPENREADINGFLAME0咖ENCODINGAFUSIONPROTEINOF1440AMINOACIDRESIDUESOFHELICASEANDRNADEPENDENTRNAPOLYMERASERDRPOFRNAVIRUSWASREVEALEDTHEGENECOATPROTEINAMINOACIDSSEQUENCEWASNOTINCLUDEDINTHISSEQUENCEBASEDONTHECHARACTEDSTIESOFPUTATIVEFUSIONPROTEINTHE64KBDSLLNAELEMENTCOULDBEREGARDEDASMYCOVIRUS，ANDNAMEDASSCLEROTINIASCLEROTIORUMHYPOVIRULENCEASSOCIATEDVIRUSSSHV3BOTHTHENUCLEOTIDESEQUENCEANDPUTATIVEAMINOACIDSEQUENCEWERESUBJECTEDTODOHOMOLOGYSEARCHWITHBLASTPROGRAMONHTTP／／WWWDDBJNIGACINTHERESULTREVEALEDTHATTHEOBTAINEDNUCLEOTIDESEQUENCEHADTHECHARACTERISTICOFPLANTPOTEXVIRUSSUBSEQUENTLYTHEPUTATIVEPROTEINCODEDBYTHE64KBDSRNAWASHIGHLYSIMILARTOTHOSEPROTEINSCODEDBYPOTEXVIRUSSUCHASBAMVBAMBOOMOSAICVIRUS，PVXPOTATOVIIUS蜀，CIYMVCLOVERYELLOWMOSAICVIRUSANDTUVXTULIPVIRUS均ETA1THERESULTSUGGESTEDSSHVMIGHTDERIVEFROMPLANTVIRUSORSHOWEDTHESAMEANCESTORWITHPLANTVIRUS4BASEDONTHEEDNASEQUENCEOFSSIWSPECIFICPRIMERSWASDESIGNEDTODETECTDSR_NAINEPIPNANDITSDERIVATIVESUSINGRTPCRTHERECOVEREDCULTURESINCLUDTHEEASCOSPOREPROGENIESEP一1PNAAEP1PNABANDEPIPNAE，THEEPROTOPLASTREGENERATIONCULTURES∞PIPNPL4，EP1PNP94ANDEP1PNL31ANDONESINGLESCLEROTIUMISOLATION2

簡(jiǎn)介：浙江大學(xué)碩士學(xué)位論文葫蘆科植物病毒研究Ⅰ主要病毒鑒定與基因組分析Ⅱ病毒生態(tài)學(xué)和致病性研究姓名陳潔云申請(qǐng)學(xué)位級(jí)別碩士專業(yè)微生物學(xué)指導(dǎo)教師陳集雙20030501略語(yǔ)表AMP氮芐青霉索鈉鹽BP堿基對(duì)BSA牛血清蛋白BU束狀體CI柱狀內(nèi)含體CP外殼蛋白DDH20雙蒸水DEPCH20DEPC處理水DSRNA雙鏈RNAEDLA已二胺四乙酸ELISA酶聯(lián)免疫吸附測(cè)定ICTV國(guó)際病毒分類委員會(huì)IPL’G異丙基硫代．B一阱半乳糖苷KB千堿基對(duì)KDA千道爾頓NT核苷酸ORF開(kāi)放閱讀框PCR聚合酶鏈?zhǔn)椒磻?yīng)PVP聚乙烯吡咯烷酮PW風(fēng)輪狀體卟PCR逆轉(zhuǎn)錄聚臺(tái)酶鏈?zhǔn)椒磻?yīng)SDS十二烷基磺酸鈉SR卷筒體UTR非編碼區(qū)XGAI薯F寢’3。吲噼臥畔乳V¨IAMPJCILLINSODIUMSALTB∞EPAIRBOVJNESE門(mén)MAIBUMJNBUNDIECYLINDERICALINCLUSIONCOATPROTEINDOUBLEDJSTILIEDWATERDISTILIEDW蹴RTREATEDWITLLDEPCDOUBLE．S訂卸DEDRNAE岫LENEDJ姍JNETE打AACETICACIDER嗎恤ELJNKEDIMMUNOSORBENT邪SAYINCEMATIONA【∞MM；CTEEONTAXONOMYOFVJLLISESISOPROPYLMIOBDGAJACTOSIDEKIIOB鶴EPAIRKJIODALT∞NUCIEIOTIDESOPENREADING療1MEPOLYMER勰ECHAINREACTIONPOIYVINYLP”ROIJDONEPINWHEEIREVER北咖SCRIPTJONPOIYMERA轉(zhuǎn)CHAJDREACTI冊(cè)SODIUMDODCCYLSULFATESCM¨UNN鋤SIA鈀D障GION5．BMM一．CHOIORO_3INDOLYLBD驢L孔TOSIDC

簡(jiǎn)介：山東農(nóng)業(yè)大學(xué)碩士學(xué)位論文中國(guó)傳染性法氏囊病病毒野毒株致病性的流行病學(xué)研究姓名孫淑紅申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師崔治中200361山東農(nóng)業(yè)人學(xué)碩JJ學(xué)位論文2003IBDVVVIBDVELDJ。RTPCRSPFDEPCDDH0PBSSDS英文縮略表傳染性法氏囊病病毒超強(qiáng)毒傳染性法氏囊病病毒雞胚半數(shù)致死量反轉(zhuǎn)錄聚合酶鏈反應(yīng)套式PCR無(wú)特定病原微生物焦碳酸乙二脂超純水磷酸鹽緩沖液十二烷基硫酸鈉

簡(jiǎn)介：上海交通大學(xué)碩士學(xué)位論文雞傳染性支氣管炎病毒保守基因片段的克隆及分子生物學(xué)檢測(cè)技術(shù)的研究姓名張斌申請(qǐng)學(xué)位級(jí)別碩士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師華修國(guó)朱建國(guó)20040101上海交通大學(xué)碩士研究生畢業(yè)論文II14天M41組可從腎臟氣管肺臟和盲腸扁桃體中檢測(cè)到IBV存在肝臟不能檢出T株組可從腎臟肝臟肺臟和盲腸扁桃體中檢測(cè)到IBV氣管未檢出H120組只能從腎臟檢出其它臟器未檢出H52組從腎臟氣管和肺臟中可檢出其它臟器未檢出上海株組只能從盲腸扁桃體和腎臟中檢出IBV攻毒后21天可從M41和T組中腎臟檢出IBV攻毒后28天后從各組各臟器中均未能檢出IBV存在對(duì)照組均未檢出IBV這為研究IBV感染動(dòng)物模型積累了一定的資料本研究還對(duì)一株雞傳染性支氣管炎病毒上海分離株SH的核蛋白基因進(jìn)行RTPCR和序列測(cè)定分析并對(duì)其編碼蛋白進(jìn)行了分析結(jié)果如下對(duì)上海分離毒株進(jìn)行RTPCR特異性擴(kuò)增獲得長(zhǎng)約12KB的特異性條帶對(duì)上海分離株進(jìn)行序列測(cè)定結(jié)果表明該片斷含有IBV核蛋白基因全序列其結(jié)構(gòu)基因全長(zhǎng)1227BP編碼409個(gè)氨基酸將上海分離株核蛋白基因序列與已經(jīng)在GENBANK中注冊(cè)的IBV其它毒株進(jìn)行同源性分析結(jié)果表明SH株與M41H120GX298SAIBWJZJ971VICS株的核蛋白基因同源性分別為87588291287385和872,將SH株的氨基酸序列與參考毒株的氨基酸序列進(jìn)行同源性比較結(jié)果如下M41株897H120株902GX298株912SAIBWJ株897ZJ971株89VICS株909本研究豐富了IBV分子流行病學(xué)資料為進(jìn)一步研究IBV的分子水平檢測(cè)方法研制IBDNA疫苗提供了基礎(chǔ)材料關(guān)鍵詞雞傳染性支氣管炎病毒核蛋白基因NESTEDPCR

簡(jiǎn)介：中國(guó)農(nóng)業(yè)大學(xué)碩士學(xué)位論文屠宰豬肝臟人病毒性肝炎的病原學(xué)初探和病理學(xué)研究姓名許江城申請(qǐng)學(xué)位級(jí)別碩士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師佘銳萍20040601ABSTRACTWEDESIGNEDTHESEEXPERIMENTSINORDERTOKNOWTHEMEETHYGIENESITUATIONANDDETECTVHLHEPATITISINTHESLAUGHTEREDSWINETHESERESULTSWILLBEHELPFULTOTHEADMINISTRATIONOFS、ⅣINESLAUGHTEREPIDEMIOLOGYANDVACCINESTUDYOFTHEVIRALHEPATITISWEHOPETHATTHERESEACHERSHOULDADVERTTOTHISPROBLEMWEDESINGED7EXPERIMENTSTOPROVIDEENOUGHEVIDENCE1SEROLOGYDETECTIONINSLAUGHTEREDSWINE2HISFILOGYANDPATHOLOGYSTUDYINSLAUGHTEREDSWINE3HBCAGANDHBSAGIRNMUNOHISTOCHEMICALDETECTIONINSLAUGHTEREDSWINE4APOPTOSISDETECTIONINSLAUGHTEREDSWINE5ELECTRONMICROSCOPEDECTIONINSLAUGHTEREDSWINE6HBVDNAPCRDETECTIONINSLAUGHTEREDSWINE7HCVIMMTMOHISTOCHEMICALDETECTIONINSLATLGHTEREDSWINE。RESULTTHEREARETOTAL136LIVERSAMPLESOFSLAUGHTEREDSWINE，ITWASFOUNDTHAT48CASESOFALIDETECTEDSAMPLES35％WEREPOSITIVEFORBOTHTHEHBSAGANDHBCAG25CASESF18％1APPEAREDPOSITIVEHBCAG，BUTNEGATIVEHBSAGINTHESE船VANTIGENHBSAG，HBCA曲POSITIVESAMPLES，WEUSEANTIBCL2／13AXANDTUNELMETHODTODETECTAPOPTOSISINTHESELIVERSAMPLESRHEREARE8SAMPLESANTIBAXPOSITIVEWITHBEL2NEGATIVE3SAMPLESANTIBD2POSITIVEWITHBAXNEGATIVENETUNELRESULTISTLLESALNEASANTIBAXRESULT8SAMPLESCORRESPONDINGPOSITIVE，PCRRESULTSHOWSDOPOSITIVESAMPLESINTHESELIVERSSOMEVIRUSPARTICLESALEALSOFOTRADEDUNDEREMTHEREARE5SAMPLESPOSITIVE8％OFTOTALTHESERESULTSINDICATETHATTHEREISHEPATITISVIRUSESPECIALLYHBVINLTHESESLAUGHTEREDSWINE’SLIVERS，BUTNOTTHESAMEASTHEHUMANHBVWESUGGESTTHATTHEPUBLICHEALTHDEPARTMENTANDHEPATITISRESEARCHERMUSTPAYATTENTIONTOTHISPROBLEMKEYWORDSSLAUGHTEREDSWINE，IMMUNOHISTOCHEMIEALMETHOD，APOPTOSIS，VIRALHEPATITIS，PATHOLOGY

簡(jiǎn)介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文黑龍江省部分地區(qū)PED流行特征及病毒生物學(xué)特性研究姓名郎景華申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師李一經(jīng)仇波20031201STUDIESONTHEEPIDEMICCHARACTERISTICSOFPORCINEEPIDEMICDIARRHEAINSOMEAREASOFHEILONGJIANGANDTHEBIOLOGICALPROPERTIESOFITSPATHOGENABSTRACTPORCINEEPIDEMICDIARRHEAPEDISAHIGHLYCOOTAGINUSENTERICDISEASEOFSWINECHARACTERIZEDBYVOMITINGDEHYDRATIONANDAHIGHMORTALITYINPIGLETS11LEDISEASEHASBEENFOUNDINMANYCOUNTRIESINCLUDINGCHINA，ANDCAUSESECONOLILICLOSSESINPIGFARMINGINTHEPRESENTSH|DY∞ACCURATEDIAGNOSISHASBEENMADEFORFIVEPIGFARMSTHATOUTBROKEVIRUSASSOCIATEDDIANHNINPIGSONTHEBASEOFCLINICALDIAGNOSISPCRDETECTIONANDVIRUSISOFATIONANDTWOOFTHEMWEREIDENTIFIEDASPEDVINFECTEDFARMS11”TESUITSFROMTHECLINICALDAMSHOWEDTHATTHEMORBIDITYRATESARE7269％AND8995％INSUDDEDPIGLETS6807％AND75％INGROWINGANDFATTENINGPI貉WHILETHEMORTALITYRATESARE6288％AND8131％INSUCKLEDPIGLETS831％AND1037％INOTHERAGEGROUPSRESPECTIVELYONTHETWOFARMSALTHOUGHSWINEOFALLAGESARCSUSCEPTIBLETOTHISVIRALM危C的NTHEMOFT8LITYINPIGLETSUNDER4WOEKSISCA3MFTLONLYOBSERVEDN把OUTBREAKSUSUALLYOCCURDURINGTHEWMTERASWELLASTHEEARLYSPRINGANDTHEMAJORCLINICALSIGNSCANBEOBSERVEDASSEVEREWATERYORYELLOWISHDIARRHEAISOLATIONANDIDENTIFICATIONOFVIRUSWEREMADEOFTCONTINUOUSVEROCELLSLINEFECALSPECIMENWHICHAREPOSITIVEFORAMPL心YLQGMGANEOFPEDVWITHUESTEDPCILWMINOCULATEDONTHECELLSANDTHEBLINDPASSAGESWEREⅦ弧KNAKENUNTILTHECPESCOULDBE毹蜘ON18PASSAGES11”CELLSB∞OMCROUNDEDLYSEDANDOFFTHEWALLOFTLIEVESSELSFORTESTIFYINGTHEPRESENCEOFPEDISOLATEONVETOCELLSRTPCRTECHNIQUEWASUSEDTOAMPLIFYMGENEATTHE5，10，15AND20PASSAGES，ANDASPECIFICFIAGMENTOFPEDVMGENEWASAMPLIFIEDTHERESULTSHOWEDTHATTHEISOTATEHASADAPTEDTOVETOCELTSTWOPAIRSOFPLIMERSWEREDESIGNEDACCORDINGTOTHEPUBLISHEDMGENESEQUENCESOFPEDVCV777ANDBRL／87SUAINSWITHOLI9041ANDELIMER50S011IFEAPREDICTED6SLBPFIAGMENTOFTHEMGENEOFPEDVWASAMPLIFIEDFROMTHERNACXLR嘶EDFROMTHEFECALSAMPLESBYRT“％睡PCI乙ANDTHENCLONEDINMTHEPMDL8TVECTOR111ECOMPLETESEQUENCESOFTHEMGANEOFTHEPEDEPIDEMICSTRAINIS681NUCLENTIDESENCODES226AMINO∞IDSN“SEQUENCESANALYSISRESUITSINDICATEDTHATTHEMMLEOTIDESEQUENCEOFPED∞IDEMICUALFTHAS9809％HOMOLOS_“INCOMPARISONWITHTHATOFCV777。EXC≈PTFOR13NUCLCOTIDEDIFFERENCESINTHESITESOF37。47，138，179，192，213，269，285，348，529，579。618AND629ANDSHARE9794％HOMOLOGYCOMPAREDTOBRL／87EXCEPTFOR14NUCLENTIDEDIFFMMLCESINTHEVL

簡(jiǎn)介：揚(yáng)州大學(xué)博士學(xué)位論文鵝源新城疫病毒部分生物學(xué)特性鑒定及其囊膜糖蛋白基因序列分析姓名萬(wàn)洪全申請(qǐng)學(xué)位級(jí)別博士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師劉秀梵20020501揚(yáng)州大學(xué)博士學(xué)位論文胞、網(wǎng)狀細(xì)胞、巨噬細(xì)胞的胞漿內(nèi)。在感染鴨的氣管、肺、食道、腺胃、法氏囊、腎臟、哈氏腺等組織中亦能檢測(cè)到病毒抗原，持續(xù)時(shí)間多為11D～15D，氣管、腎臟則可達(dá)19D以上，個(gè)別感染鴨的腸道里微弱的陽(yáng)性反應(yīng)；在肝臟、胰腺、脾臟、腦和心肌組織中未檢測(cè)到病毒抗原。結(jié)合免疫組化染色強(qiáng)度、病毒抗原存在時(shí)間及相應(yīng)組織病變的嚴(yán)重程度進(jìn)行比較發(fā)現(xiàn)，鵝源NDV對(duì)鵝、鴨的致病性和組織親嗜性存在較大差異。在體外試驗(yàn)中，本試驗(yàn)研究了鵝源NDV在雞胚成纖維細(xì)胞CEF培養(yǎng)物上的致病變特性和增殖動(dòng)態(tài)，并將其與雞源、鴿源NDV的相應(yīng)特性進(jìn)行了比較。結(jié)果，鵝源NDV、雞源NDV及鴿源NDV感染CEF次代培養(yǎng)物后，在24H即開(kāi)始有病變產(chǎn)生，最初各種毒株導(dǎo)致的病變相似，表現(xiàn)為細(xì)胞變圓、皺縮、折光性增強(qiáng)，但鵝源毒株感染的細(xì)胞單層病變進(jìn)展緩慢，表現(xiàn)為細(xì)胞逐漸地變圓、浮起、崩解，并伴有大量合胞體形成在84～144H單層破壞、消失。麗雞源、鴿源毒株接種的細(xì)胞單層病變進(jìn)展迅速，在36一48H即見(jiàn)單層有因細(xì)胞壞死、脫落而導(dǎo)致的空白區(qū)產(chǎn)生，60～84H單層即徹底破壞。對(duì)病毒接種后不同時(shí)間培養(yǎng)上清HA效價(jià)的測(cè)定結(jié)果表明，鵝源NDV接種后84～120H培養(yǎng)上清HA效價(jià)達(dá)到高峰，為5～8L092，而雞源、鴿源NDV接種后60～84H上清HA效價(jià)即達(dá)到高峰，但其最高效價(jià)只有4LO啦。本研究結(jié)果表明鵝源NDV可以在感染鵝的多種組織細(xì)胞中復(fù)制，導(dǎo)致以變性、壞死為主的廣泛的組織損傷；鴨對(duì)鵝源NDV具有較強(qiáng)的抵抗力，但病毒可以在感染鴨的一些組織中復(fù)制鵝源NDV在體外具有比雞源和鴿源毒株更強(qiáng)的導(dǎo)致細(xì)胞融合的能力。2鵝源新城疫病毒融合蛋白基因部分片段的克隆及序列分析用RTPCR技術(shù)擴(kuò)增NDVF基因的部分片段，經(jīng)克隆、測(cè)序獲得了11株鵝源NDVF基因的部分5’端1700個(gè)核苷酸M序列，分析測(cè)定的序列及推導(dǎo)的相應(yīng)氨基酸序列，并對(duì)鵝源NDV的基因型分類地位進(jìn)行探討。結(jié)果表明，除J蚍，98／GO株以外，11株病毒中有LO株病毒F基因的核苷酸同源性大于97％，JS／2／98／GO株與這10株病毒F基因核昔酸的同源性為907～912％。11株病毒與標(biāo)準(zhǔn)強(qiáng)毒株F49E8F基因的同源性為853％～868％。

簡(jiǎn)介：學(xué)校名稱垡主壅些盔堂學(xué)校代碼Q5Q皇丫662045分類號(hào)密級(jí)豬流感病毒的分離鑒定、HA基因的克隆序列分析和血清學(xué)研究SWINEINFLUENZAVIRUSISOLATIONANDIDENTIFICATIONCLONINGANDANALYSINGOFTHEHAGENEANDSEROLOGICALSTUDIES研究生導(dǎo)師指導(dǎo)小組伍銳金梅林教授陳煥春院士金梅林教授陳煥春院士郭愛(ài)珍教授何啟蓋副教授吳斌副教授方六榮副教授專業(yè)預(yù)防獸醫(yī)學(xué)研究方向病毒性傳染病學(xué)位級(jí)別碩士中國(guó)武漢二OO四年五月ABSTRACTSWINEINFLUENZAISAHIGHLYINFECTIOUSRESPIRATORYDISEASECAUSSEDBYSWINEINFLUENZAVIRUSAFFECTINGSWINEOFDIFFERENTSTAGES，SEXESANDBREEDSSWINEINFLUENZAWASFIRSTREPORTEDINUSAIN1918SHOPEISOLATEDANDIDENTIFIEDH1N1SUBTYPESWINEINFLUENZAFROMSWINEIN1930THEDISEASERESULTSECONOMICLOSEANDTHREATSANIMALHUSBANDRYANDHUMANHEALTHATPRESENT，ITWIDELYTAKESPLACEINMANYNATIONSANDREGIONSINTHEWORLDTHERESEARCHOFSWINEINFLUENZAHASBECOMEAHOTTOPICNOWADAYSSWINEINFLUENZABROKENINOURCOURTRYIN2002OURLABORATORYCARRIEDOUTISALATIONANDIDENTIFICATIONFROMCOLLECTEDCLINICALSAMPLESTHREESIVH3SUBTYPESTRAINSWEREIDENTIFIEDUSINGSTANDARDDIAGNOSTICMETHODSBESIDESBIOLOGICALCHARACTERIZATIONSWERESTUDIEDTOTHESEVIRUSTHESEISOLATESCANAGGLUTINATEREDBLOODCELLSCOMINGFROMDIFFERENTANIMALS，BUTTHEIRHEMAGGLUTININWERENOTSTABLETHESEISOLATESWERESENSITIVETOPHYSICALANDCHEMICALFACTORS，BUTWERESTRONGLYRESISTANTTOD刪ANDCOLDTHESEISOLATESAREHIGHLYINFECTIOUSTOCHICKENEMBRYOTHESEMICEANDPIGSEXPERIMENTLYINFECTEDWITHSWINEINFLUENZADISPLAYEDCLASSICRESPIRATORYSYNDROMESANDVISIBLEPATHOLOGYTHESWINEINFLUENZAVIRUSTHEFULLHALGENEWASAMPLIFIEDBYRTPCRWITHPRIMERSDESIGNEDSPEFICTOCONSERVEDHAREGIONTHERESULTSINDICATEDTHATTHREESWINEINFLUENZAHADHIGHLYHOMOGALARSEQUENCESWITHH5SUBTYPEAVIANINFLUENZAVIRUSMEANTIMETHERESULTSHOWEDTHATFUJIANISOLATEBELONGSTOTHESAMEBRANCHASAIVINDIRECTENZYMELINKEDIMMUNOSORBENTASSAY伍L(zhǎng)ISAOFSIVWASESTABLISHEDFORDIAGNOSESIVTHEBESTWORKCONDITIONWASDONE，MEANTIMESPEFLCITYANDREPRODUCIBILITYTESTWERECARRIEDOUTTHERESULTINDICATEDSIVELISAWEESTABLISHEDHASGOODSPEFLCITYANDREPRODUCIBILITYANDCANBEAPPLIEDTODIAGNOSESWINEINFLUENZATHERESULTSINDICATEDTHATOURNATIONHADEXISTEDANDPREVAILEDSWINEINFLUENZA，THATSWINEINFLUENZAHADBECOMESERIOUSDISEASETHROUGHOURASSAYMETHODWHICHHADBEENESTABLISHEDWITHTHEINCREASINGDANGEROFMULTIINFECTION，THESEROLOGICALSTUDYWASBEDONETHERESULTSSHOWEDSWINEINFLUENZAANDPCVORAPPHAVEHIGHLYINFECTIOUSPERCENT，THOUGHSWINEINFLUENZAANDPRRSHADLOWLYINFECTIOUSPERCENTKEYWORDSSWINEINFLUENZAVIRUS；ISOLATEANDIDENTIFY；BIOLOGICALCHARACTERIZATION；HAGENESWINEINFLUENZASERAEPIDEMICSURVEY

簡(jiǎn)介：中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文甜菜黑色焦枯病毒和煙草壞死病毒的比較病毒學(xué)研究姓名席德慧申請(qǐng)學(xué)位級(jí)別博士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師于嘉林李大偉20040601中國(guó)教監(jiān)大學(xué)博士學(xué)位論文ABSTRACTABSTRACTLHAVESTUDIEDTHEBEETBLACKSCORCHVIRUS，XINJIANGBBSVXANDNINGXIABBSVNISOLATESANDHAVEFOUNDPATHOGENICITYDIFFERENCESBEDNCONTHESETWOSTRAINSTHECOMPLETECDNACLONEOFBBSVXWASOBTAINEDBYRTPCROFTHEXINJTANGRNACOMPARISONSBETWEENBBSVXANDBBSVNREVEALEDANUCLEOTIDESEQUENCEIDANTITYOF98，9％，ANDTHEGENOMICSTRUTTUREWASALMOSTUNIFO巾1HOWEVER，THREEADDITIONAINUCLENTIDESWEREINTERSPERSEDBETWEENNUCLEOTIDES889AND975WITHINORF2INBBSVXTHESETHREENUCLEOTIDEDIFEE佗NCESRESULTEDINAFRAMESHIFFDIFFEREOCEINCOMPARISONSWITHTHEBBSVNSTRAINTHATAFFECTEDAMINOAEIDS28510312OFTHEP82PROTEINWHICHMAYBETHEREPLICASEASSOCIATEDPROTEINTHEAMINOACIDSEQUENCEIDENTITYOFTHEP82PROTEINSOFBBSVXANDBBSVNWAS954％IALSOCONSTRUCTEDANINFECTIOUSCLONEOFBBSVXBYPLACINGTHECD_NAUNDERTHECONTROLOFTHE啊PROMOTERBASEDONTHEINEECTIOUSCDNASOFBBSVNANDBBSVXIEXCHANGEDPARTIALFRAGMENTSOFTHEP82GENEAFTERINOCULATINGCHENOPODIUMAMARANTICOLORANDVIGNASINENSISWITHINVITROTRANSCRIPTS，MTRANTSRESULTINGFROMTHEP92FRAGMENTEXCHANGESANDWILDTYPEVIRUSWEREOBSERVEDFORPHENOTYPICCHANGES，BUTTHESYMPTOMSANDHOSTRANGEWERENOTALTEREDTHUS，THEREAPPEARST0BENODIRECTREL撕ONSHIPBETWEENTHEVARIATIONOBSERVEDINP82ANDTHEPATHOGENICITYOFTHETWOBBSVISOLATESANINFECTIOUSELONEOFTHESATRNAOFBBSVXWASALSOCONSTRUCTED塒PLACINGTHECD_NAUNDERTHECONTROJOFTHE35SPROMOTERTHISPLASMIDCOULDINFECTCHENOPODIUMAMARANTO如RWITHTHEHELPOFINVITROTRANSCRIPTSOFBBSVXSIXDIF托RENTDELETIONANDINSERTIONMUTANTSOFBBSVXWERECONSTRUCTEDANDTHESEWEREINOCALATEDTOCHENOPODIWNAMARANTICOLOR11地PRELIMINARY船ULTSSHOWTHATTHESATRNACOULDENHANCESVMPTOMOFBBSVXANDITSJNSERTIONMUTANTSONCHENOPODIWNAMARAMICOLORBUTTHEPATHOGENICITYOFTHECPDELETIONMUTANTS蟠REDUCEDINORDORTODETERMINETHERELATIONSHIPBETWEENBBSVANDOTHERNECROVIRUSMEMBERS，INCLUDINGTHETYPESPECIESTOBACCONECROSISV0USTNV，IALSOSTUDIEDTHEBIOLOGICAL，PHYSICAL，CHEMICALANDSEROLOGICALPROPERTIESOFTHECHINASOYBEANISOLATEOFTNVR簿DATAP讒SENTEDBELOWINDICATE也缸THISISOLATEISANEWSTRAINOFTNVAWHICHIHAVED髓IGNATEDNⅣA。1K1NV小ISOLATEHASAHI曲STABILITYINVITROANDALSOHASABROADHOSTRANGEINCLUDING29SPECIESIN8FAMILIESTNVA。GENERALLYINDUEES10COLLESIONSINMOSTHO啦BUTSOYBEANANDⅣBENTHARAIANAALESYSTEMICHOSTSIPURIFIEDTHEVIRUSANDTHENOBTAINEDAHIGHTITER_NQVA。ANTISERUMTHERESULTSOFSEROLOGICALSTUDIESSHOWEDTHATTNVA。ISCLOSELYRELATEDTOTHETNVWILLOWISOLATEBUTFAILSTEACTWITH“INVDANDBBSVBASEDOBTNVSEQUENCESREPORTEDPREVIOUSLY，LDESIGNEDDEGENERATEPRIMERSANDOBTAINEDSUBCLONESREPRESENTINGPORTIONSOFTHETNVA。GENOMETHECOMPLETESEQUENCEOFTHEISOLATEWASDETERMINEDBY5“RACEAND3’RACETHEDATAREVEALTHATTHEVIRUSENCODESFIVEORFSINA3682NTGENOME，ANDTHATTHENUELEOTIDESEQUENCEIDENTITYISRESPECTIVELY864％437％AND443％BETWEENTHE1NVA。ISOLATEANDTHOSEOFTNVA，TNVDANDTNVD“WHOSESEQUENCESHAVEBEENREPORTEDPREVIOUSLYTHUSACCORDINGTOTHEBIOLOGICALPROPEAIESMADNUCTEOTIDESEQUENCESOFTHESESTRAINSIPOSTULATETHATTHESOYBEANISOLATEISANEWSTRAINOFTNVATOPRODUCEINFECTIOUSCDNACLONESTNVA。CDNAWASPLACEDUNDERTHECENTRELOFTHET7ANDTHE35SPROMOTERS，ANDDEIETIONMUTANTSINOR1，ERR3，ORF4ANDORF5WEREGENERATEDTHROUI曲ANALYZINGTHESYMPTOMSANDGENOMERNAREPLICATION，ICONCLUDETHATTHEORFIGENEISTHEREPLICASEASSOCIATEDGEREANDTHATORF3ANDORF4ARECELLTOEELLMOVEMENTGENESTHEPROTEINENCODEDBYORF5AFFECLSLONGDISTANCEVIRUSMOVEMENTWHICHRESULTSINATWODAYDELAYOFLESIONAPPEARANCEINCHENOPODIUMAMARANTICOLORKEYWORDSBEETBLACKSCORCHVIRUSPATHOGENICITYDIFFERENCE，TOBACCONECROSISVIRUBIOLOGICALPROPERTIES，INFECTIOUSEDNACLONE11

簡(jiǎn)介：浙江大學(xué)碩士學(xué)位論文植物病毒侵染寄主的分子細(xì)胞學(xué)研究ⅠBBWV2在寄主體內(nèi)的復(fù)制和轉(zhuǎn)運(yùn)機(jī)制探討ⅡSCMV和SRMV的細(xì)胞病理變化比較姓名王衛(wèi)兵申請(qǐng)學(xué)位級(jí)別碩士專業(yè)植物病理學(xué)指導(dǎo)教師洪健20040501SCMV引起風(fēng)輪體、卷簡(jiǎn)體和片層聚集體，屬于EDWARDSON等劃分的第1II型，一些成束的病毒粒子和內(nèi)含體存在于篩管中。SRMV引起風(fēng)輪體和卷簡(jiǎn)體，屬于第1型，一些內(nèi)含體分布于細(xì)胞壁附近，有的直接與細(xì)胞壁胞間連絲相接，完整的柱狀內(nèi)含體存在篩管中，推測(cè)內(nèi)含體在病毒的胞間運(yùn)動(dòng)中起定位和傳輸作用。兩種病毒在細(xì)胞病理學(xué)效應(yīng)方面的差異也可以作為診斷鑒定的一個(gè)參考依據(jù)。關(guān)鍵詞蠶豆萎蔫病毒；甘蔗花葉病毒；高粱花葉病毒；細(xì)胞病理學(xué)；超微結(jié)構(gòu)膠體金；內(nèi)含體管狀結(jié)構(gòu)；電子顯微鏡

簡(jiǎn)介：廣西大學(xué)碩士學(xué)位論文廣西豬繁殖與呼吸綜合征病毒的分子生物學(xué)研究姓名蔣小紅申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)學(xué)指導(dǎo)教師余克倫黃偉堅(jiān)20040501廣西大學(xué)2004屈碩士畢業(yè)論文苷酸及其推定的氨基酸的同源性分別為938100％和952100％，與LV株的核苷酸及其推定的氨基酸的同源性分別為663％和639％。同源性分析顯示，GXA株與VR一2332、MLV和BD一4的同源性非常高，而GXA與LV同源性非常低。構(gòu)建的系統(tǒng)發(fā)育樹(shù)分析，GXA與VR一2332、MLV及BJ4株親緣關(guān)系比較密切。從而表明GXA株屬于美洲型毒株，可能來(lái)源于疫苗株。將GXA株的E、M、N基因從重組質(zhì)粒PMDE、PMDM、PMDN中亞克隆至原核表達(dá)載體PET32A上，構(gòu)建了原核表達(dá)重組質(zhì)粒PETE、PETM和PETN，并通過(guò)酶切鑒定，證實(shí)構(gòu)建成功。關(guān)鍵詞豬繁殖與呼吸綜合征病毒PRRSV分離GXA株克隆測(cè)序同源性原核表達(dá)重組質(zhì)粒RESEARCHONMOLECULARBIOLOGYOFPORCINEREPRODUCTIVEANDRESPIRATORYSYNDROMEVIRUSPRRSVINGUANGXIABSTRCTINRECENT，SOMEPIGFARMSINGUANGXIOCCURFREQUENTLYANINFECTIOUSDISEASE，WHICHCHARACTERSAREABORTION，DEATHOFEMBRYO，MUMMIFIEDFETUSES，ANDRESPIRATORYPROBLEMSINPIGLETPATHOGENOFTHEDISEASEWASCONSIDEREDASPORCINEREPRODUCTIVEANDRESPIRATORYSYNDROMEVIRUSPRRSVINTHISSTUDY，WEIDENTIFIEDTHATTHESAMPLECOLLECTEDFROMTHEFARMINNARMINGWASPOSITIVEBYRTPCRWITHAPAIROFSPECIFICNGENEPRIMERSOFPRRSVBASEDONIT，PERMISSIVECELLS，MARC一145CELLSWEREINOCULATEDWITHTHESAMPLEAFTERSIXBLINDPASSAGES，TYPICALCYTOPATHOGENICEFFECTCPEWASOBSERVEDONCELLSASWELLASTHEPOSITIVECONTR01ANDPRRSVWASPOSITIVEINTHECELLSANDCULTUREMEDIUMBYRTPCRTHEFIRSTPRRSVSTRAININOUANGXIWASISOLATEDSUCCESSFULLY，NAMEDGXASTRAINANDITSINFECTIONTITERWAS105”TCID5N／M1

簡(jiǎn)介：山東農(nóng)業(yè)大學(xué)碩士學(xué)位論文山東省H5N1和H9N2亞型豬流感病毒的分子流行病學(xué)調(diào)查姓名許傳田申請(qǐng)學(xué)位級(jí)別碩士專業(yè)預(yù)防獸醫(yī)指導(dǎo)教師趙宏坤范偉興20040526山東農(nóng)業(yè)大學(xué)碩L學(xué)位論文中文摘要近年來(lái)，山東省各地豬場(chǎng)不斷發(fā)生以呼吸道癥狀為主要表現(xiàn)的疾病，給養(yǎng)豬業(yè)造成了嚴(yán)重的損失，這些呼吸道疾病包括藍(lán)耳病、豬喘氣病、豬圓環(huán)病毒、豬流感等，有時(shí)為兩種或兩種以上的病原同時(shí)感染，為了弄清豬流感病毒在引起豬呼吸道疾病中所起的作用，20022003年我們?cè)谏綎|省20多個(gè)發(fā)病比較典型的豬場(chǎng)展開(kāi)對(duì)豬流感病毒的分離鑒定和分子流行病學(xué)的調(diào)查。首先在各疑似豬流感發(fā)病地區(qū)進(jìn)行采樣，按常規(guī)把病料處理之后接種SPF雞胚，在雞胚中進(jìn)行連續(xù)傳代，每代無(wú)菌收獲雞胚液，用雞或豚鼠的紅細(xì)胞進(jìn)行血凝和血凝抑制試驗(yàn)來(lái)驗(yàn)證是否有流感病毒，待傳代后雞胚液的血凝價(jià)較高并比較穩(wěn)定后再用標(biāo)準(zhǔn)血清進(jìn)行定型。對(duì)初步確診為H5和H9兩個(gè)豬流感病毒株進(jìn)行了電鏡觀察，并作了小自鼠攻毒和豬體回歸試驗(yàn)來(lái)驗(yàn)證這兩個(gè)毒株的致病性。對(duì)山東10個(gè)發(fā)病豬場(chǎng)的30份血清進(jìn)行檢測(cè)，H5和H9的抗體檢出率為30％，盡管抗體水平不高由哈爾濱獸醫(yī)研究所流感中心代測(cè)，所測(cè)的血清中有H3和HL的豬流感病毒的抗體，但本次流感病毒分離中沒(méi)有分離到H3和H1亞型。在細(xì)胞上觀察H9和H5豬流感病毒引起的致細(xì)胞病變效應(yīng)CPE，山東分離株H5和H9流感病毒能在雞成纖維細(xì)胞、HELA細(xì)胞，MDCK，以及MDBK上產(chǎn)生的CPE。分別挑選一株H9N2和H5N1亞型典型毒株進(jìn)全基因克隆測(cè)序，利用DNASTAR軟件進(jìn)行序列分析，并和GENBANK中的禽、人和豬的核苷酸同源性分析和氨基酸形成的進(jìn)化樹(shù)進(jìn)行分析，同時(shí)對(duì)HA的氨基酸裂解位點(diǎn)

簡(jiǎn)介：V762‘5C奶牛持續(xù)性感染口蹄疫病毒的分子生物學(xué)檢測(cè)導(dǎo)師FII建民教疆“孔飪州究日Ⅱ，M覽膏Q基礎(chǔ)獸畦學(xué)0，0礎(chǔ)免＆學(xué)沈日衣_E上學(xué)攢簧大小約為838BP戇基因片段。DNA廖列分輯表甓，該部分基因與嗣態(tài)搬遂的0型FMD瘸海抹L冬基鞭瓣澡毪這88％。葒結(jié)聚為持續(xù)毪爨染F鬻蚤V靜分予浚幸亍瘸學(xué)誕壹提供了襖據(jù)。關(guān)鍵溺；爨蹲痰FMDV；逆轉(zhuǎn)豢聚會(huì)薅鑣式茨應(yīng)；RNA搓鞭；滓歹分新

簡(jiǎn)介：Y9500．70分類，J8526密級(jí)單位代碼1Q盟學(xué)號(hào)?S03，盟QQ8名微雇業(yè)大學(xué)學(xué)位論文安徽省豬圓環(huán)病毒病分子流行病學(xué)調(diào)查T(mén)HEINVESTIGATEOFMOLECULAREPIDEMIOLOGYOFPCV2INANHUI研究生指導(dǎo)教師合作于}}導(dǎo)教師申請(qǐng)學(xué)位門(mén)類級(jí)別專業(yè)名稱研究方向所在學(xué)院揚(yáng)世基魏建蠱副煎援塹疊羞熬援叢生塹』麴隨盞匡堂苤查籃鎏瘟堂塾塑型拉堂醫(yī)答辯委員會(huì)主席透鑫支又和DQL95679及AY556476、AY556473三個(gè)毒株關(guān)系較近，而與一株美國(guó)株AY325495、一株福建毒株AY556474共同組成的一個(gè)分支相距較遠(yuǎn)。關(guān)鍵詞豬圓環(huán)病毒II型，分子流行病學(xué)，調(diào)查Ⅱ

簡(jiǎn)介：中山大學(xué)碩士學(xué)位論文蛙病毒分子流行病學(xué)調(diào)查及其對(duì)中華鱉致病性和防治姓名游靖申請(qǐng)學(xué)位級(jí)別碩士專業(yè)水生生物學(xué)指導(dǎo)教師何建國(guó)20050530RANAVIRUSMOLECULAREPIDEMIOLOGYANDSTUDYONTHEPATHOGENICITYOFRANAVIRUSAFFECTINGONTRIONYXSINENSISASTRACTRANAVIRUSWASIDENTIFIEDASTHEPATHOGENCAUSINGTHEFLOGSANDTHEOTHERAQUATICANIMALSMORTALITIESINCOMMERCIALCULTURESINCHINAINOURSTUDY4PAKSOFPRIMERSWEREDESIGNEDACCORDINGTHESEQUENCESOFTWOFUNCTIONALGENESINTIGERFROGVIRUS，NⅣ，WHICHGENOMESEQUENCEHADBEENTESTEDBYOURLABORATORYANDSENSITIVENESTEDPCRPROCEDURESAREMODIFIEDINTHEEXPERIMENTTODETECTTHE10。4PGGENOMEDNAOFTFVINTISSUETHENTHESENSITIVEPATHOGENSPECIFICNESTEDPCRDETECTIONPROCEDURESAREAPPLIEDTOINVESTIGATETHEMOLECULAREPIDEMIOLOGYOFTHERANAVIRUSINTHEAQUATICANIMALSINSIXCITIESOFGUANGDONGPROVINCEINTHEINVESTIGATION，WEFOUNDTHELATENTAFFECTIONWASVERYHIGHABOVE20PERCENTINTHETIGER的GSRANATIGRNARUGULOSA，THEAMERICAFLOGSFR口NEHECKSTHERIANDTHECHINESESOFTSHELLTURTLESTRIONYSSINENSISANDWEDRAWACONCLUSIONTHATTHEIRIDOVIRUSCAMEFROMBOTHTHETIGERFROGSANDTHECHINESESOFTSHELLTURTLESMAYBEONESPECIEBYTHEPHYLOGENETICANALYSISOFTWOFUNCTIONALGENESFORFURTHERSUBSTANTIATEWHETHERTRIONYSSINENSISCOUMHEAFFECTEDBYTF址WEINJECTEDTHEVIRUSWHICHCAMEFROMCELLCULTURETHEMAFTER78DAYSOBSERVATION，THEMORTALITYRATEWAS7080％ATTHETEMPERATUREOF25℃THEREISTHEREDABDOMINALSHELLDISEASEINTHESURFACESYMPTOMASBOTHTHEPCRANDINSITUHYBRIDIZATIONVIRUSDETECTIONS，EVERYVISCERACANBEAFFECTEDBYTHEVIRUSESWHENTHECHINESESOFTSHELLTURTLESWEREIMMUNIZEDWITHTHEINACTIVATEDVIRUSVACCINE，THEPROTECTIONRATESWERE70％IIL20DAYSOBSERVATIONKEYWORDSRANAVIRUS，NESTEDPCR，MOLECULAREPIDEMIOLOGYPHYLOGENETICANALYSIS，VACCINE，INSITUHYBRIDIZATIONⅡ