尾穗莧再生體系的建立及茉莉酸甲酯對維生素E合成的影響研究.pdf

1、華南理工大學碩士學位論文尾穗莧再生體系的建立及茉莉酸甲酯對維生素E合成的影響研究姓名：劉南波申請學位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：鄭穗平20090401濃度MeJA對四種TCPs的生物合成均有不同程度的抑制，而致使TCPs總水平較顯著降低。關(guān)鍵詞：尾穗莧：再生；愈傷組織；不定芽；茉莉酸甲酯AbstractAmaranthuscaudatusLinn，asoneoftheessentialgrainspecieofAmaranthus

2、，showsaverypromisingnutritionalandeconomicpotentialbecauseofitshighproteincontentontheaveragewithawellbalancedaminoacidcomponentwithahighlysineandmethioninecontent，andCanbeusedasagoodfeedresourcesUptonowlimitedworkhasbee

3、ndoneonthetissuecultureofAmaranthuscaudatusLinnInordertoexploitthisnutritionresourcesandimprovethevitaminEqualityinthispaperweconductedarangeofexperimentsaimedattheestablishmentahigh—frequencyregenerationsystemofplantsfr

4、omdifferentexplantsofAmaranthuscaudatusLinn，aswellasastudyontheeffectsofMeJAtreatmentonthesynthesisofvitaminE(tocopherols)inthissystemThemainresultsaresummarizedasfollows：(1)Optimizationofinfluentialfactorsofseedgerminat

5、ion：theoptimizedfactorsforseedgerminationWasusing01％HgCl2tosterilizeseedsforImin，1／2MS(halfofallelements)mediumanddarkincubationforonedaybywhichtheseedgerminationrateontheseventhdaycouldreach827％(2)Callusinductionandorga

6、nogeneticprocess：theoptimummediumforcallusinductionfromhypocotylandeotyledonWasMS05mg／L6BA05mg／L2，4D，onwhichthecallusinductionrateWas100％，andthequalityofcallusfromhypocotylswagbetterthanthatfromcotyledonShootsregeneratio

7、nfromcallusfromhypocotylWasnotobserved，andsomeredgranuleswereformedoncallusculturedonMS20ppm6一BA02ppm2，4一D，buteventuallyfailetegenerateshoots(3)Directdifferentiationandregenerationsystem：explantssuchashypocotyl，cotyledon

8、andleafofAmaranthuscaudatusLinnnevershowedshootsregenerationinpresenceofthosedesigneddifferentiationmediums，instead，lotsofcalluswereformedShootWassuccessfullyinducedfromeotyledonarynodesexplant，seedlingstageandcuttingway

9、sdidnotshowdistinctinfluencesShootscouldsuccesfullybeinducedfromcotyledonarynodesonlyby6BA，butshootsgrewslowlyandweaklyWhencultruedinpresenceof6BAplusNAA，highqualityshootswereinduced，andtheoptimumculturemediumWasMS10megL