2,3-吲哚醌抗人神經(jīng)母細(xì)胞瘤SH-SY5Y細(xì)胞侵襲及遷移機(jī)制研究.pdf

1、 分類號：R739.4 密級：不保密 UDC UDC：610 學(xué)校代碼：11065 碩 士 學(xué) 位 論 文 2，3-吲哚醌抗人神經(jīng)母細(xì)胞瘤 吲哚醌抗人神經(jīng)母細(xì)胞瘤 SH SH-SY5Y SY5Y 細(xì)胞侵襲及遷移機(jī)制研究 細(xì)胞侵襲及遷移機(jī)制研究 徐平平 指 導(dǎo) 教 師侯琳

2、 教授 學(xué) 科 專 業(yè) 名 稱 生物化學(xué)與分子生物學(xué) 論 文 提 交 日 期 2 0 1 5 年 5 月 1 1 日 論 文 答 辯 日 期 2 0 1 5 年 6 月 3 日 答辯委員會主席 劉均洪 教授 Abstract Background: Isatin is widely present endogenously in both human and other mammalian tissues and

3、 fluids likely as a result of the tryptophan metabolic pathway. Isatin showed significant inhibiting effect on the growth of SH-SY5Y neuroblastoma cells. The objective of this study was to investigate that isatin is also

4、 able to alter the invasion and migration ability of SH-SY5Y cells and probably mechanism. Methods: SH-SY5Y cells were exposed to isatin at various concentrations (100, 200 and 400 μmol/L). Invasion assays were conducted

5、 to detect the invasiveness and scratch assay was used to analye metastasis. MMP2 and MMP9 mRNA was analyzed by Real Time RT-PCR. MMP2, MMP9 and pSTAT3 protein were analyzed by Western blotting. Results: Isatin caused th

6、e most significant inhibition of cell migration and invasion. The inhibition rates of invaded cells were 42.14 % and 87.42 %, respectively (P<0.01). Wound-healing assay showed that cell migration was reduced in 200 μm

7、ol/L group compared with the control group at 36 h and 48 h, respectively. The inhibition rates of migrated cells were 42.78 % and 45.03 %, respectively (P<0.05). Treated with isatin, the expression levels of MMP2 and

8、 MMP9 mRNA and protein was decreased (P<0.05). Moreover, phosphorylated STAT3 was decreased in SH-SY5Y cells with isatin in a concentration-dependent manner (P<0.01). Conclusions: Isatin can inhibit migration and i

9、nvasiveness probably by reducing MMP2 and MMP9. The reason leading to these alterations might be that isatin down-regulates the level of pSTAT3. Postgraduate student: Xu Pingping (Biochemistry and Molecular Biology) Dire