豬水腫病大腸桿菌毒力基因突變菌株對豬的免疫效果研究.pdf

1、ⅱ2.2.3突變菌株的定植檢測..................................................272.2.4攻毒保護試驗.......................................................273.結果.......................................................................293.1微囊的制備

2、.............................................................293.1.1微囊形態(tài)與結構.....................................................293.1.2微囊的耐人工胃酸試驗................................................303.1.3微囊的人工腸液溶出試驗.........

3、.....................................303.1.4微囊產品的貯存性能..................................................313.1.5微囊的安全性評價...................................................313.2免疫效果檢測........................................

4、...................313.2.1SLTIIeA蛋白的表達和純化...........................................313.2.2免疫效果測定.......................................................353.3突變菌株的定植檢測.....................................................4

5、43.4攻毒保護實驗...........................................................463.4.1大腸桿菌SLTIIe的提取及檢測........................................463.4.2臨床表現(xiàn)...........................................................473.4.3眼觀病變.......

6、....................................................473.4.4病理組織學變化.....................................................484.討論.......................................................................524.1微囊制備方法的選擇..........

7、...........................................524.2突變菌株在仔豬腸道內的定植.............................................534.3黏膜免疫與口服疫苗.....................................................534.4仔豬口服突變菌株O139免疫效果評價........................

8、...............545.結論.......................................................................56致謝.........................................................................57參考文獻.............................................