具有抑菌活性成分中草藥的篩選及防腐保鮮應(yīng)用機(jī)理研究.pdf

1、華南理工大學(xué)碩士學(xué)位論文具有抑菌活性成分中草藥的篩選及防腐保鮮應(yīng)用機(jī)理研究姓名：賴毅東申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：寧正祥20030501華南理工大學(xué)工學(xué)碩士學(xué)位論文AbstractThirtyspeciesofChineseherbalmedicineswereextractedwithethanolandtheroughextractsweretestedfortheirantimicrobialandfungitoxi

2、tyactivityonsevenspeciesmicroorganismsThemethodofinhibitoryareawasusedtotesttheinhibitoryeffectsofthirtyspeciesofplantextractsonmicroganismsandsporegerminationofAspergillusnigerandPeronophythoralitchichenResultsshowedtha

3、tfivespecies，RhizomaAcoriRhizoneEugeniacaryophyllataThumb，爿fpiniaofficinarumHanceCinnamomumJaponicum，Rheumpalmatum上havethebestantimicrobialandfungitoxityeffectThefivespecies’ethanolextractshaveshowedstronginhibitoryeffec

4、tsinmycelialgrowthinhibitiontestandinhibitoryareatestfortheirinhibitoryeffectsonsporegerminationofAspergillusnigerandPeronophythoralitchichenalsoshowstornginhibitoryeffectTheinhibitionrateofthefivespecieswereabove85％Thre

5、especies’ethanolextracts，RhizomaAcoriRhizone，EugeniacaryophyllataThumb，CinnamomumdaponicumshowedcompletelyinhibitionrateonmyeelialgrowthandthezoosporesreleaseofPeronoph)’thoralitchichenshowedequivalentstronginhibitoryeff

6、ectswiththechemicalcontrol02％sodiumbenzoateTenmatchingliquorsweregeneratedbytheabovefiveextractionsInhibitoryeffectsofextractionsandmatchingliquorsweremadebymeansofthemiddleinhibitoryconeentration(MIC)，thetestsshowedthee

7、thanolextractsofclove(TW15)andthematchingliquor，PW6havethestrongestinhibitoryeffectonmycelialgrowthandthezoosporesreleaseofPeronophythoralitchichenMICofPW15ishalfofTW15ThephysicalandchemicalcharactsofTW15andPW6weretested

8、afterbeingtreatedatdifferenttemperature，illuminationandpHvaluesAndthetimelengthsforpreservationandtheactivitymaintenancetimelengthsoftwoliquorsTheresultsshowedthatmatchingliquorPW6havebettereffectsthanTWl5inthesetestingi

9、temsTheinhibitoryactivityofTWI5decreasedsharplywhentemperaturehigherthan60℃，TW二l5andPW6remainedmostactiveatpH，bothincreasinganddecreasingofpHcausedthedroppingdownofinhibitoryactivityAftertreatedwithilluminationfor72h，bot

10、hofextractshadnosignificantchangeintheirinhibitoryactivitiesThepreservativeexperimentresultsofTW15andPW6onpeargrapeandlitchishowedthattwopreservativeliquorshavereallysignificantfresh—keepingeffectsComparedtothecontroltes