eNOS基因轉(zhuǎn)染兔腦動脈預(yù)防蛛網(wǎng)膜下腔出血后遲發(fā)性腦血管痙攣的實(shí)驗研究.pdf

1、南昌大學(xué)醫(yī)學(xué)院碩士學(xué)位論文eNOS基因轉(zhuǎn)染兔腦動脈預(yù)防蛛網(wǎng)膜下腔出血后遲發(fā)性腦血管痙攣的實(shí)驗研究姓名：伍偉俊申請學(xué)位級別：碩士專業(yè)：外科學(xué)（神經(jīng)外科）指導(dǎo)教師：錢鎖開20100601AbstractABSTRACTObjective：Toinvestigatetheeffectsofintravasculartransfectionofendothelialnitricoxidesynthasegeneoncerebralvasospa

2、smaftersubarachnoidhemorrhageinrabbits，andexploretherelatedmechanismthroughobservatingpathohistologyandultrastructuralstructureofcerebralarteriesandhippocampusMethods：Recombinantadenovirusvectorscarryingendothelialnitric

3、oxidesynthasegenewereestablishedDelayedcerebralvasospasmmodelafterSAHWaSdevelopedbyinjectingarterialbloodtwicethroughcistemamagnaRecombinantadenovirusWaSinjectedintocarotidarterialtOtransfecttherabbitsRabbitswererandomly

4、dividedintofourgroups：normalgroup：nobloodWasinjectedintocistem；SAHgroup：injectingarterialbloodtwicethroughthecistemamagnaafter0and48hours；Adsgroup：injectingadenoviruswithouteNOStotheSAHrabbits；AdeNOSgroup：injectingAdeNOS

5、tOtheSAHrabbitsAllanimalswerekilledusingperfusion—fixationonday7andthebrainarteriesandhippocampaltissuewereremovedforpathohistOlogyandultrastructuralstudiesImmunohistochemicalmethodwasusedfordetectingtheexpressionofeNOSR

6、esults：Indoublehemorrhagemodle，bloodaccumulatedinthesubarachnoidspace，especiallyinthebasalcisternImmunohistOchemistryconfirmedthatrecombinanteNOSgeneexpressedmainlyintheendotheliumUndermicroscope，theaveragediametersofthe

7、cerebralarterialinAdeNOSgroupwerelargerthanSAHgroupandAdsgroup，andtherewaslessvasospasmunderelectronmicroscopeSAHgrouphadhippocampaltissueedemaandwidenedperivascularspaceThenumberofneuronsofhippocampalCA1regioninAdeNOSgr

8、oupismorethanSAHgroupSAHgrouphadmarkedpathologicalchangesofneuronultrastructureincludingcellswelling，structuralintegritynuclearcondensationmitochondrialvacuolizationConclusions：Thisstudysuggeststhatcontinuoususeofcarotid