基因芯片研究肝硬化和原發(fā)性肝癌的基因表達(dá)譜.pdf

1、華中科技大學(xué)同濟醫(yī)學(xué)院碩士學(xué)位論文基因芯片研究肝硬化和原發(fā)性肝癌的基因表達(dá)譜姓名：黃海濤申請學(xué)位級別：碩士專業(yè)：消化內(nèi)科指導(dǎo)教師：林菊生2002.5.1華中科技大學(xué)同濟醫(yī)學(xué)院碩士畢業(yè)論文GeneexpressionprofilingofcirrhosisandHepatcellularcarcinomawithcDNAmicroarraysThepathogenesisofhepatitisBvirus(HBV)associatedci

2、rrhosisandhepatocellularcarcinomainvolvesmanygenesfrommultiplepathogenicpathwayseDNAmicroarraycontaining1，568geneswasusedtocomprehensivelyanalyzethegeneticalterationsinHCCandlivercirrhosisAmongthe1，568targetgenes，therewe

3、re6676(42％48％)geneswhoseexpressionleveldifferedin2casesand28(18％)differentiallyexpressedgenesin3casesbetweenthenormalandcirrhotictissuesand19genesdifferentiallyexpressedbetweenthecirrhoticandHCCtissuesHierachicalclusteri

4、ngwasperformedtoidentifygenesrelatedtoclinicalphenotypesoftheHCCpatients12genesshowedchangesassociatedwiththedegreeofdifferentiationofHCCOurpresentanalysisclarifiedanumberofgenesthatcharacterizelivercirrhosisandHCCTheres

5、ultobtainedfrommicroarraymaybehelpfulindiagnosisforHBVassociatedcirrhosisandhepatocellularcarcinomaandinidentificationoftargetgenesfordesigningrationaltherapeuticstrategiesFurtheranalysisofthesedifferentiallyexpressedgen

6、eswillbehelpfulforunderstandingthemolecularmechanismoflivercirrhosis，itsmalignantchangeandclarificationofthemechanismofhepatocarcinogesisKeywords：eDNAmia[oim職hepatocellularcarcinoma；livercinhosis；differentlyexpressedgene