藠頭離體培養(yǎng)技術(shù)的研究.pdf

1、浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院碩士學(xué)位論文藠頭離體培養(yǎng)技術(shù)的研究姓名：閆淼淼申請學(xué)位級別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：郭得平20080501浙江大學(xué)碩士學(xué)位論文菖頭離體培養(yǎng)技術(shù)的研究AbstractChinesejiaotou0IliumchinensisGDon)hasbeencultivatedvegetativelybecauseofsterility，lowpropagationrate，andalaborious，time—cons

2、umingprocedureand，asaresult，viralinfectionconstitutesaveryseriousprobleminitscultivationTissueculturetechniqueprovidesausefultoolforimprovingtheefficiencyofpropagationandeliminatingvirusesfrominfectedplantletsandforprodu

3、cingvirusfreeChinesejiaotouseedlings，whichimprovestraditionalChinesejiaotoubreedingprogramsandproducescultivarswithhigheryields，highertolerance／resistancetoviraldiseasesTherefore，weintendedtoevaluatetheeffectsofdifferent

4、explantsandgrowthregulatorsoncallusinductionandplantregenerationinthisplantThemostimportantfactorsaffectingplantregenerationaretheexplanttype，thephysiologicalconditionoftheexplantandthegrowthregulatorcombinationusedinthe

5、culturemediumInthisexperiment，theexplantsuchasbasalplate，root，leafandflowerwereused，thegrowthregulator6BAand24一DandtheircombinationsweretestedonthecallusinductionandshootregenerationTheresultsindicatedthatbasalplatewassu

6、itableexplantforinvitrocallusinductionandregenerationTheB5mediumsupplementedwithO1mg／L6BAand10mg／L24一Dprovedtobeoptimummediumforcallusinduction，andthebestcallusproliferationwasobservedwhenculturedonB5mediumsupplementedwi

7、th15mg／L24一DThehighestnumberofadventitiousshootswasachievedonB5mediumsupplementedwith01mg／L6BAand10mg／LNAAThehighestfrequencyofcallusinductionwasobservedonmediumcontaining10mg／L24D6BAcouldpromotethefrequencyofcallusinduc

8、tionandO1mg／L6BAwasfoundtobemostsuitableconcentrationforcallusinductionHighconcentrationof24一DwasnotonlyeffectiveoncallusinductionbutalsofavoredthemaintainceofproliferationanddifferentiationofcullusproducdHigherNAAconcen

9、trationresultedinthehigherfrequencyofshootdifferentiationfromcallusEffectofdifferentphysiologicalage，inducedtimeandplantgrowthregulatorsoninvitrodifferentiationandregenerationofflowerorganofAlliumchinensiswereinvestigate

10、dinthisexperimentResultsfromthisstudydemonstratedthatonlythosebudsofsmallandmediumsizecoulddifferentiateThefrequencyofadventitiousshootproductionwashigherwhenthesegmentsofovariesexplantsculturedfor15doninductionmediumThe