LTD4對K562細(xì)胞增殖和凋亡的影響.pdf

1、華中科技大學(xué)碩士學(xué)位論文LTD4對K562細(xì)胞增殖和凋亡的影響姓名：郭冬梅申請學(xué)位級別：碩士專業(yè)：臨床檢驗(yàn)診斷學(xué)指導(dǎo)教師：李慧玉201105華中科技大學(xué)碩士學(xué)位論文華中科技大學(xué)碩士學(xué)位論文3LTD4MediatesProliferationApoptosisinChronicMyelogenousLeukemiaCellsK562InVitroAbstractObjective：Bonemarrowmicroenvironment(BM

2、M)notonlysuppthematopoieticcellsgrowthdifferentiation，butalsocontributetotumcellsgrowthBMMplayacriticalroleinproliferation，differentiationapoptosisoftumcells.LTD4existsinBMMinthepresentstudyweinvestigatedtheeffectsofLTD4

3、onproliferationapoptosisofchronicmyelogenousleukemiacelllineK562cellsindertoprovidetheeticalsupptfanewstrategyonreversingdrugresistancethetherapyofleukemiabymeansofmodifyingtheleukemichemopoieticmicroenvironment.Methods:

4、1.Bonemarrowstromalcellsweregrownfrombonemarrowmononuclearcellsobtainedfrom6casesofnmal6casesofleukemiaindividualsthecontentofLTD4insupernatantfrommarrowstromalcellcultivationwasinvestigatedbyenzymelinkedimmunoadsdentass

5、ay(ELISA).2.HumanleukemiacellslineK562cellswerecultured.directlystimulatedwithdifferentconcentrationsofLTD4f48hinvitro.CCK8assaywasusedtoexaminetheeffectofinducinggrowth.AnnexinVPIstainingwasusedtodetecttheeffectofapopto

6、sis.TheexpressionsofrelatedgenemRNAwasdetectedbyRTPCR.Results:1.ThemeancontentofLTD4insupernatantfromleukemicstromalcellculturewas1684.5185.1pgmlsignificantlyhigherthanthatfromnmalstromalculture(1192.589.6pgml)（P0.05）.2.