前列腺癌演變過程中的等位基因失衡.pdf

1、山西醫(yī)科大學碩士學位論文前列腺癌演變過程中的等位基因失衡姓名：張海峰申請學位級別：碩士專業(yè)：病理學與病理生理學指導教師：路喜安20100515山西醫(yī)科大學碩士學位論文Abstractobjecti、，e：TbinVeStigatethegeneticbasisoftheclonaleVolutionofprostatecarcinomaMethods：1’wentyfivcc雒eswe陀∞lected矗0mdepanmentofpamo

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3、elclosspattemwe他prep卸edfomprima巧tumors鋤dmatchedsynchronouslymphnodemet嬲ta略esbyl雒盯capturemicrodissection(LCM)TheoligonucleotideprimerpairSfomthemicorSatelliteDNAmarkerS、VereD8S133，D8S136，D8S137，ANKlonchromosome8p1221，LPLT

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7、iIIlbal鋤cebe№npriIIm巧齜ldmetasEatictumorSatanymarkeranalyzed(PO05)ConcllIsions：1Pr0蛐carcinomdeVel叩wimmeaccumulationofgeneticabnomalitiesT|lehi曲骶quencyofL0ssofHeter0巧gosity(LOH)0n8phaVebeenfoundbothinprima巧鋤dme切statictumor

8、smmisstudynsu髂eStsthatone鋤dmoreunidentifiedTSGsare10cated0nchromosome踟8pthatplayaroleinthedeVelopmentofpr0Statecarcinoma2Theseda：taSuggeStthatdi腩remp舭nlSofallelicdeletionmaybeacquiredduringcancerprogrcssiont0metaSt嬲esThe

9、di仃erenCesiIlgeneticcompositionbe帆eenprimaD，pr0疏atecarciIloIIla鋤ditsmet嬲taSesmayberelatedt0證trinsiccancerheterogenei劬oVerallgeneticins訕訂i劬andcloIlaldive唱ence3Collectingandpuri匆ingc觚ceroustissuebyusinglasercapturemicrodis

10、sectionmethod，s吮ngtIlsliesiIlitsselectiVi劬hi曲accuracyc觚e船ctiVelypreVentotherepitllelialcellsarldsn0lnalcells111ixed、Ⅳimspecime船、^，：hjchledt0falsenegatiVeproblem，SothatresultSoftlle鋤lysisaremoreaccurateK叼啊ords：proStatec趾c