檢測糞便中K-ras突變及胰液細胞學輔以檢測抑癌蛋白表達在胰腺癌篩選和診斷中的價值.pdf

1、中國協(xié)和醫(yī)科大學博士學位論文檢測糞便中Kras突變及胰液細胞學輔以檢測抑癌蛋白表達在胰腺癌篩選和診斷中的價值姓名：徐彤申請學位級別：博士專業(yè)：消化內(nèi)科指導教師：陸星華錢家鳴2000.5.1DetectionofKrasmutationsinstoolsanddetectionofantioncogeneproductsasanadjunctincytologicexaminationofforscanninganddiagnosispu

2、repancreaticjuiceofpancreaticcarcinomaABSTRACTSBACKGROUND：1Pancreaticcarcinomaisconsideredasoneofthemostmalignanttumorinhuman，withthe5yearsurvivalratelessthan5％Inrecentyears，itsincidencewasincreasingsteadilyEarlydiagnosi

3、scanmakemoretumorresectableandimprovetheoutcomeofthisdiseaseKrasisthoughtspecificallyrelatedtothetllmorigenesisofpancreaticcarcinomaThepointmutationofcodon12inexon1ofKrasgonehavebeenidentifiedin90％ofpancreaticctrcinoma，b

4、utmuchlessinbenignpancreaticdiseaseSodetectionofKrasmutationmayassistthediagnosisofpancreaticcarcinomaOne0fourobjectsiStodetectKrasmutationsinstoolSanddisccussitsapplicationinscreeningforpancreaticcancers2!Theabnormaliti

5、esofgeneandexpressionofCDKinhibitorssuchadp53、P16Ink4、p21WallhaverecentlybeenimplicatedinpancreaticcarcinogencsisTherateofoverexpressionor10SSofexpressionoftheseantioncogeneproductsinpancreaticcarcinomawas37—79％Buttheexp

6、ressionofp27andtheinterrelationshipoftheseproteinsarestillunknown，CytologicexaminationofpurepancreaticjuicecanfindtumorCellsandhelptodistinguishbenignfrommalignantpancreaticdiseaseButthesensitivityoftheconventionalcytolo

7、gicexaminationwasunsatisfactoryTheseDondaimofthisstudyiStoinvestigatetheexpressionofantio硎cogeneproductsinpancreaticcarcinomaandbasedontheseresultstodevelopanewmethodofimmunOcytOchcmistryinconjunctionwithconventionalcyto