基于芯片技術(shù)的肥胖人外周血基因表達譜初步研究.pdf

1、北京中醫(yī)藥大學博士后學位論文基于芯片技術(shù)的肥胖人外周血基因表達譜初步研究姓名：龔海洋申請學位級別：博士后專業(yè)：中醫(yī)內(nèi)科學指導教師：王琦20040701北京中醫(yī)藥大學博士后研究工作報告ABSTRACTObjeetive：Obesityisacommonmetabolicdiseasenowadays，andisoneofthecriticalriskfactorstohumanhealthinthe21centuryTheeffectso

2、fgeneticandenvironmentonthegenerationofobesityhavebecomethefocusinobesityresearchGenechipshavebeenreportedasausefultoolinhumanadiposetissueresearchObeseandnonobesepersonsweredescribedbytraditionalChinesemedicine口CM)since

3、ancienttimesIt’sthefirsttimethattheperipheralbloodgeneexpressionprofileofChineseadultobesitieswasstudiedbygenechiptechniqueThistechniqueprovidestheoreticalsupportforthediscriminationofTCMobeseandnonobesepersonsfortheprev

4、entionandtreatmentofobesity■Methods：Fivecasesofobesepatients(4maleandlfemale，aged214士o9years)andthreecasesofnonobesepersons(2maleandlfemaleaged26蛸3years)wet@selectedbytheinernationalobesitystandardTheregularphysicalexami

5、nation，TCMsymptomsandsyndromes，dietaryhabitsofthepatientswereinvestigatedaccordingIyTotalRNAwasextractedfromperipheralblood，anditwasamplifiedandlabeledThequalityandquantityoftheprocessedsampleswerecheckedbyA母metrixhumang

6、enomeTest3arrayThegeneexpressionprofilesweremonitoredbyAffymetrixhumangenomeU133AsetThedatawasanalyzedusingAffymetrixMicroarraySuiteVersion50AffymetrixMicroDBVersion20，AffymetrixDataMiningToolVersion30andSASONEWAYANOVATh

7、egenesymbolswithsignificantdifferenceweresearehcdinGENEBANKdatabaseResults：Theresultsshowedthatthebodyweightbodymassindex∞IMXobesegrade，pu№，systolicbloodpressureanddiastolicbloodpressureoftheobesepatientsweresignificantl

8、ybgherthanthoseofthenonobesecontrolsComparewiththenonobesepersons，theperipheralHoodgeneexpressionlevelsweredifferentintheobesepatientsTherewele212genesexpressionupregulatedand251genesexpressiondownregulatedinwhich96genes

9、weroupregulatedabove2folaand109genesweredownregulatedabove2foldTheresultsdemonstratedthatthefollowinggenesupregulatedabove4fold：GYPA，CRATCRI，CHRNB4，MGC3771，PKLR，MAPK8IP3，CHDCl，CRBl，DKFZP434N1923andKIAAl598；whileG。FSEIand

10、NAALAD2weredownregulatedabove4foldHLADQAIandLOC201895wereevenshowntobeupregulatedabove10foldConclusions：ThepetipheralbloodgenedifferentialexpressionprofilesinChineseadultobesepatientswerefoundforthefirsttimeItisvaluablef