食管鱗狀細胞癌和宮頸鱗狀細胞癌中9號染色體等位基因雜合性缺失的比較研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文食管鱗狀細胞癌和宮頸鱗狀細胞癌中9號染色體等位基因雜合性缺失的比較研究姓名：馬海霞申請學(xué)位級別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：王全紅20090316山西醫(yī)科大學(xué)碩士學(xué)位論文ComparativeresearchoftheLOHallelomorphicgeneonchromosomeybet、ⅣeenesophagealsquamouscarcmomaandcelwlcalSCluamouscarcinomaAbst

2、ractobjective：TocomparetheLOHrateofallelomorphicgeneonchromosome9attheidenticaldistrictandsitebetweenesophagealsquamouscarcinomaandcervicalsquamouscarcinomatoexploretherelatedantioncogeneincommonMethods：LOHofsevenmicrosa

3、tellitemarkersonchromosome9weredetectedin45casesofesophagealsquamouscarcinomaand46casesofcervicalsquamouscarcinomabymierodissectionPCRdenaturingpolyacrylamidegelelectrophoresis，silvernitratestainingandradiographtechnolog

4、y111erelationofLOHrateandclinicalpathologyparameterwereanalyzedusingxtestbySPSS130softwareResults：①Intheinformativecases，thetotalfrequencyofLOHinesophagealsquamouscarcinomaWas258％，LOHWasdetectedasf0110ws：43％atD9S753，38％a

5、tD9S303，36％atD9S171，36％atD9S162，17％atD9S242，17％atD9S1748and0％atD9S43T11etotalfrequencyofLOHiIlesophagealhighgradeintraepithelialneoplasiawas164％，LOHWasdetectedatmarker308％atD9S303，286％atD9S171，286％atD9S753，151％atD9S162，1

6、11％atD9S242，67％atD9S1748and0％atD9S43(9Intheinformativecases，thetotalfrequencyofLOHincervicalsquamouscarcinomawas252％，LOHWasdetectedasfollows：407％atD9S171，333％atD9S43，314％atD9S162，237％atD9S242，172％alD9S303167％atD9S753and1

7、29％atD9S1748ThetotalfrequencyofLOHiIlcervicalhighgradeintraepithelialneoplasiawas159％，LOHWasdetectedatmarker296％atD9S171，229％atD9S162，20O％atD9S43，172％atD9S303，125％atD9S753，105％alD9S242andO％atD9S1748③ThereWasnosignificant

8、associationbetweenLOHrateatsevenmicrosatellitemarkersandgenderhistopathologicgrade，theinformationoflymphadenmetastasisConclusions：①Thestudyinsevenmicrosatellitemarkersindicmesthatthereisaccumulationofantioncogeneabnormal

9、ityduringtheprocessfromnormalsquamousepitheliumtohighgradeintraepithelialneoplasiaandesophagealsquamouscarcinomaandcervicalsquamouscarcinoma②Theabnormalityofp15ispossiblyassociatedwiththedevelopmentandprogressionofesopha

10、gealsquamouscarcinomaandcervicalsquamouscarcinoma③TherearecommonantioncogenepossiblyatorReal“D9S162whichrelatedtotheprogressionofesophagealsquamouscarcinomaandcervicalsquamouscarcinoma@D9S303andD9S753on9q13—223possiblyre