酶解玉米蛋白提取ACE抑制肽的研究.pdf

1、分類號(hào)浮卻t。乏碩士學(xué)位論文題目：酶解玉米蛋白提取ACE抑制肽的研究密級(jí)英文并列題目：墅!!型!!竺翌塵!星蘭堡!盟塑墮壘墾里!些!堡i塑型旦121i魚望魚!坐曼!!坐墮!!!竺!Y!墮!研究生：劉萍專業(yè)研究方向：食品生物技術(shù)食品科學(xué)導(dǎo)師：楊嚴(yán)俊教授指導(dǎo)小組成員學(xué)位授予日期答辯委員會(huì)主席：夏文水教授江南大學(xué)地址：無錫市二oo六年穴月英文摘要AbstractACE(An2iotensin—I—ConvertingEnzyme)，isane

2、nzymewhichexistintheendodermiscellofhumanbloodvesselItplaysanimportantroIeintheadiustInentofbJoodpressureWhenitisinhibited，thebloodpressLlrewiIldropTodavalotofsyntheticalACEinhibitorhavebeenmade，buttheirinhjbitoreff爸cton

3、ACEarereversjbIeSo。theeffbctcannotlastforlongtimeanddm2withdrawlcancausetherebounceofb】oodpressureWhat’smore，thesvntheticalmedicinecandoha肌forrenalfunctionSo，itismeanin2fultofjndanewkindofmedicinefromfoodresourceswhichis

4、safeeⅡ色ctualandinnoXiousCGMfComGlutenMeal)isthemainoutgrowthjntheproducingofcomstarchItcontainsabout60％protein，mainlyofzeinTherearehighproportionofhydrophobeaminoacidandpm，glu，but10wproportionofalkalescenceaminoacidsucha

5、slysinCOMSuchidiographicaminoacidmal(eupofCGMmadeitIownutritionvalueandlowsoJubility，butgoodresourcesformaI】ykindsofbioactivepeptides，especiallyforACEinKbitor)，p印tidesInthispaper，CGMwasusedassubstratetopmduceACEiflhibito

6、rypeptidesPretreatmemofsubatrateisnecessaryastherearestillmanyinlpurityinthea3MFiveor2anicsolventwastestedinthedecolorexperiment，and95％etlanolwasselectasbestsolventARerhvdroIyzedby口一artlylaSe，盧一amyIase，mestarchwaSmostlyr

7、emoved舶mtheCGM，andtheproponionofproteininCGMincreasedfrom6154％to8572％ReductaJlt，pHandtemperaturecanallafrectthesolubilityofCGMThesolubilityofCGMincreasedf沁m420％to3096％aRerheated15minutesat90℃ontheconditionofDH10～12with01

8、0％Na2SO，PretreatedCG】Ⅵwashydrolyzedbysingleandcomplexproteaserespectivelvin7kindsofproteaSesforaIltihypenensivepeptidesPeptidesactivitywasdetenninedbyCuslmlanUltra—violetspectrophotometerThehydrolyteobtainedbyAlcalase24L

9、proteaseshowedthehi2hestactivity，andtheoptnnumconditionsofhydrolysisofCGMwasdetennindedbyexperimenttobe：【S】=25％，【E]／【s]=48Au／l(g，pH=8O，T=55～60℃Thehydml”ewimhvdrolysisdegreeof1596％boastthehighestjnhibitoryactivitytoACE，an

10、ditsIC50was0247mg／mLARertheDuri行cationofthehydrolytewithultrafiltration，BioradP一2Gelnltrationchromatogratphy，andRP—HPLC，anactivefractionwithanIC50ofO085m∥mLwasobtained，theIC50ofwhichis344％oftheoriginalhydr。lyteThesequenc

11、eoflhefractionwasfoundtobeAlaTvraRertheapplicationofaminoacidcompositionassavandthecombinationofliquidchromat02raphywithmassspectrometrYAnditsICsowas2269umol／LTheIC50ofAlaTvrhardlychan疊edaRerincubatedwith30muACEat37℃for3