孕牛乳汁中早孕因子的分離純化及部分特性的研究.pdf

1、東北農業(yè)大學碩士學位論文孕牛乳汁中早孕因子的分離純化及部分特性的研究姓名：張宏玲申請學位級別：碩士專業(yè)：臨床獸醫(yī)學指導教師：于文會20080620AbstractEarlypregnancyfactorSeparation，PurificationandIdentificationfromPregnantCows’MilkAbstractTheearlypregnancyfactor(EPF)WaSseparatedandpurifie

2、dbycelluloseionexchangechromatography，sephadexgelchromatographyandaffinitychromatographfrommilkofpregnantCOWS，tOgainthehighlypurifiedEPF，andtrytomakeantiEPFmonoclonalantibodyatfirststageExperimentalMethods：Milkoffourmont

3、hpregnantCOWSWaSgatheredanddegreasedbyhypothermyfreezingcentrifugationThedegreasedmilkwasseparatedandpurifiedbyammoniumsulfatefractionationsalting—out，DEAE52celluloseionexchangechromatography，G—100dextrangelchromatograph

4、y，ConASepharoseFF6BaffinitychromatographytechnologyinturntoobtainEPFofhighpurityTheEPFactivitiesofvariousstagesweredetectedbyrosettesuppressiontestMolecularweightofEPFwasexaminedbySDS—polyacrylamidegelelectrophoresis1soe

5、lectricpointofEPFwasexaminedbypolyacrylamidegelisoelectricfocusingelectrophoresisExperimentalResults：Afterion—exchangechromatographyandrosettesuppressiontest，theEPFactivityofeveryreceivedpeakwasdetected，withtheresultthat

6、peakofEPFactivityisDE—IAfterdextrangelchromatographyandrosettesuppressiontest。t11eEPFactivityofevel’yreceivedpeakwasdetectedwiththeresultthatpeakofEPFactivityisG111C—Icomponentof06800mgWasobtainedbyaffinitychromatography

7、technologyfrom400mlmilkofpregnantCOWSTwoproteinbandscanbeseenafterSDS—polyacrylamidegelelectrophoresiswiththerelativeolecularweightof2I0kDa，670kDaandisoelectricpointof680Conclusion：Thisresearchindicatedthattheseparationa

8、ndpurificationtechnical1outeofearlypregnancyfactorisfeasible，earlypregnancyfactorsofhighpuritywereobtainedKeywords：pregnantcows；milk；earlypregnancyfactor；separationandpurificationCandidate：ZhangHonglingSpeciality：clinica