基于凝血酶與腺苷核酸適配體功能化納米探針識別的分析方法研究.pdf

1、江南大學碩士學位論文基于凝血酶與腺苷核酸適配體功能化納米探針識別的分析方法研究姓名：彭曉麗申請學位級別：碩士專業(yè)：營養(yǎng)與食品衛(wèi)生學指導教師：樂國偉王周平20080801AbstractNanoprobetechniqueandaptamerrecognitionarenovelprobetechniqueandmolecularsrecognitionmodedevelopingrapidlyinrecentyears，whichhav

2、epresentedtremendouslypotentialintheapplicationofbiologicalanalysis，drugtesting，foodsafetydetection，clinicalverify，environmentalanalysisandtheothersLuminescnectanalysiswiththemeritsofhilghsensitivityandwidelinearrangehas

3、alsoattractedmoreandmoreattentionsrecentlyThispapermeanstoconstructnovelnanoprobesusingthrombinandadenosinebindingwiththeirspecificaptamer—functionalizedAunanoparticles，combining謝t11chemiluminescencedetectingtechnology，t

4、heAuandAgstainingsignalamplificationtechnologyandresonancelightscatteringtechnology，establishmethodssystemssuitforhighlysensitivedetectionofthrombinandadenosineInthemeanwhile，electrochemiluminesceceanalysiswascombinedwit

5、IlcapillaryelectrophoresisseparationtoestablisharapidlyandsensitivelyanalyticalmethodforclarithromycindeterminationinbiologicalfluidsAllthesewouldbeofimportanceforenrichingthetheoryandacceleratingtheapplicationsofnano—pr

6、obeandluminescentanalysistechniqueFirstly，combiningtheaptamerfunctionalizedAunanoparticleswithaptamerrecognitiontechnology，usingthrombinasthemodelanalyte，anovelanalyticalmethodforthrombinWaSdevelopedcoupling、Ⅳithflowinje

7、ctionnanogoldstrippingchemiluminescenceanalysistechniqueThedectionglimitofthismethodis03x10。10egmLSecondly，based011abovereseachusingthrombinasmodelanalyte，introducingitsspecificaptamerrecognisethrombin，thrombinspecificap

8、tamerfunctionalizedAunano—probewereaSsembledtothesolidmatrixsurfaceThenaselectivegolddepositionprocedureongoldnanoparticlessurfacewascarriedouttorealizethefirstsignalamplificationStrippingchemiluminescencedetectionWascon

9、ductedtofurtheramplifythedetectionsignals，makingthesensitivityofthebiologicalsensingsystemenhancedgreatlyThirdly，consideringtheproblemoftimeconsumptioningoldstainsimilartogoldstainingbaSedstrippingCLdetection，ChapterIVse

10、lectivelydepositesilveronthesurfaceofnanogoldforthefnstinsitusignalamplification，andthenbondwiththestrippingchemiluminescencedetectiontocarryoutthesecondsignalamplification，establishedanovelultratracedetectionmethodforth

11、rombinThedectionglimitofthismethodis03xlO‘12g／mLFinally，couplingnanoprobetechnology，aptamerrecognitionwithresonancelightscatteringanalysis，throughrecognisingreactionbetweenadenosineanditsspecificaptamerinhomogeneousmatri

12、x，causingthedeaggregationoftheaggregatednetworkstructureofadenosinespecificaptamerfunctionalizednanogoldstructure，asharplychangeontheresonancelightscatteringsignalcanbeobtainedBasedonit，anovelhomogeneousaptamerrecognizin