干擾素對HL-60細胞影響的研究.pdf

1、青島大學碩士學位論文干擾素對HL60細胞影響的研究姓名：于宏申請學位級別：碩士專業(yè)：兒內(nèi)科（小兒血液）指導教師：孫立榮20050528StudyofIFNaeffectonHL60cellsAbstract0bjectiveTodetectinvitrotheeffectofIFNainHL60proliferation、differentiationandapoptosisandtoprovidesomeexperimentalevi

2、dencesforthewidelyclinicuseofIFNincuringacuteleukemiaMethodsCulturingstandardHL一60cellstrainanddispartingonecontrolgroupandfiveexperimentalgroupsMTTwasusedtomeasuretheproliferationofHL一60cellsThecellmorphologicalchangesw

3、asobservedbystmnedwithWrightmethodTheCDl3positiveratewasdetectedbyimmunofluorencetechniqueThecellapoptosiswasinvestigatedbystainedwithAO／EBResults1、HL一60leukemiacellinthecontrolgrouphasnochangeTheODvalueofBgroupinMTThasn

4、odifferencewithAgroupfPO05)TheODvalueofCgrouphasdifferencewithAgroupfPO05)TheODvaluesofD、E、Fgroupshavehighdifferences伊O01)Andthevaluesaredecreasedwiththeconcentrationincreased2、ThecellmorphologyhasnochangesinBgroupthough

5、stainedwithWrightmethodItbeginedtochangeinCgroupandbecomeddistinctinDgroupThenucleolusreducedobviouslyThenucleoluskerneldisappearedThecellplasmbecomedmuchThenucleoluscomparedlittlewithplasmThenucleolusleanedonesidewitIlh

6、ollowanddispartedleaves3，ThepositiverateofCDl3Was65％inAgroupTheyare61％、46％，39％、32％、29％separatedlyinB、C、D、E、FgroupsTheBgrouphasnodifferencewithAgroup，howevertheothershavedifferences(P005)4、Thecellpositiveratewas28％inAgrou

7、pwimacridineorangestainedTheywere50％，56％，59％，65％，71％separatedlyinB、C，D，E、FgroupsTheyweremainlylateapoptosiscellsinE、Fgroups，howevertheywereearlyapoptosiscellsinB、C、DgroupsConclusionIFNanotonlyinhibitstheproliferationbuta