雞傳染性法氏囊病抗獨特型抗體疫苗的研究.pdf

1、東北農(nóng)業(yè)大學碩士學位論文雞傳染性法氏囊病抗獨特型抗體疫苗的研究姓名：劉懷然申請學位級別：碩士專業(yè)：預防獸醫(yī)學指導教師：李一經(jīng)2000.5.1簍絲竺。登簍塞蘭蘭壘塞窒量鍪些簍釜竺壘冀塑絲童!!!!；：StudiesonAnti—idiotypeAntibodyVaccineofChickenInfectious弘ursalDiseaseABSTRACTlnfoctiousbursaldisease(IBD)ofchickeniSOneof

2、theseriouslyinfectiousdiseasesthatdamagepoultryindustryVaccinationofroutinevaccinesiSthemajormeansforcontrolofIBDThough，theyhavesomedisadvantagesinevitablyforexample：inactivatedvaccinescouldresultinpotentialinfectionduet

3、osurvivalviruses；ailattenuatedvaccinesdoharmtochickenbursas；thewideranginguseofattenuatedvaccinescouldhampertIlecontrolofinfectiousdiseaseandCausealterationtovirusvirulenceandantigenicityTherefore，wehavethestudiesonanti—

4、idiotype(anti—lmantibodyvaccineofIBDinordertoavoidthesedisadvantagesanddevelopahovelkindofsafevaccineaswellasestablishthetheoryfoundationforit’SpracticalUSeinfutureThemulticlonalantiIdantibodywaspreparedbymeansoftraditio

5、nalinlmunoserofog弘AttenuatedD1sstrainoflBDVwerepropagatedinchickenembryofibroblastscells(CEF)andpurifiedonasucrosegradientThespecific—pathogenfree(SPF)henswereimmunizedwiththepurifiedvirusesandtheIBDVspecificyolklgG(AbI)

6、werepurifiedfromtheyolkwithtrichlommethmledegrease，sodiumsulfateprecipitationandDEAE32ionexchangefiltrationTherabbitswerejmmanizedwithAblandpartoftherabbitsefUmcontaininganti—Id(Ab2)weresaltedoutwith(NH4)2S04，thenantiIdw

7、erepurifiedwithSepharose4BaffinityfiltrationThepurifiedantiIdwasdetectedwithinhibitionandcompetitiveinhibitionELISAonimmunochemistryTheresultsindicatedthatantiIdantibodycouldcompetitivelycombinedAblwithantigen(IBDV)，buta

8、lSOinhibit|hecombinationbetweenAblandantigen，whichconfirmedthatthepurifiedanti—Idantibodyactuallycarryantigen”intemalimage”一Ab2BTheantildantibodyconfirmtoimmunechemicalpropertyofantiidletypeantibodyBiologicalexperimentso

9、fanti—Idantibodyi靠vitrodemonstratedthatanti—ldantibodycouldmimicIBDVcombiningneutralizingantibodyofIBD、，_Immuneprotectivetrialshowedthatanti—ldvaccineinducedantiIBDVantibodyandprotectedchickenagainst1BDVtosomeextentThead

10、juvantaffecteditsprotectiveefficiencyInthestudyprotectiveratioofFreund’Sadjuvant(833％)issuperiortothatofcommonwhiteoiladjuvant(615％)，andprotectiveratioofroutinevaccine(80％)iSbetterthanthatofanti—Idvaccine(695％)inover越l。P