產香蘋果酒嗜殺酵母的選育.pdf

1、山東農業(yè)大學博士后士學位論文產香蘋果酒嗜殺酵母的選育姓名：杜金華申請學位級別：博士后士專業(yè)：果樹學指導教師：束懷瑞2001.9.1AbstractTherearealotofyeastsinapplejuiceprocessingplantwherefermentablesugarisrichAfterseparatingandselectingbyfermentingexperiment，acideryeast46withgoodfe

2、rmentationcharacteristicswasselectedThe5yeaststrains46，Saccharomycescerev&iaeay—l，ay一2，ay一3anday一4wereusedtofermentapplejuicetodryandthebrothwasstoredforthreemonthsatnomore也anl0℃afterthedepositisolatedTheclearciderswereu

3、ndergoneGC，ph)7sicalandchemicalanalysisandsensoryevaluationAmongthose5strains，ay一4showedgoodcharacteristicThecharacteristicsofay～4areasfollowed：shortlagphase，quickandsmoothfermentation，lessvariationlevelofacidityandpHdur

4、ingfermentation；producinglowerfoamheadduringmainfermentation；thehighestlevelofdiacetylbeingquietlowTheciderfermentedbyay一4hadtypicalandharmoniousfruitaromaGCanalysisshowedthattherewerehilghestcontentsofnpropynol，nbutanol

5、，isobutanol，2一methylbutanol，nhexanolandethyllactatewhichwere68798mg／L、33634mg幾、215908mg／L、50271mg／L、3263mg／Land28360mg／LrespectivelyTherewerelessacidsandacetaldehyde，lowestcontentsof2phenylethanolandglycer01Aly～4wasmores

6、uitabletofermentappleciderUsingprotoplastfusiontechnique，KIkillerplasmidfromSaccharomycescerevisiae5045，amutantofSaccharomycescerevisiaedefectivefornuclearfusion，wasintroducedintotherecipientstrainay一4，adiploidSaccharomy

7、cescerevisiaeidentifiedasauxotrophicdefectiveforpantothenicacidThefactorsontheformationandregenerationoftheprotoplastofay一4werestudiedinthispapegsuchaspretreatment，betterphaseoflogarithmicgrowth，concentrationofZymolyasea

8、ndlytictime，culturalmethodforregenerationOnoptimumconditions，thecellofay一4waspretreatedwithmixedEDTAsolutionand13METsolution(v／v，25：1)for30minandthenlysedby005mg／mLZymolyasefor40～60minat30℃，asaresult，thefrequenciesofform