AA-2βG對過氧化氫誘導(dǎo)兔晶狀體混濁的保護性實驗研究.pdf

1、寧夏大學(xué)碩士學(xué)位論文AA2βG對過氧化氫誘導(dǎo)兔晶狀體混濁的保護性實驗研究姓名：周旋申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：張自萍20090401AbstractObjective：ToevaluatetheeffectofAA213GinprotectinglensagainstoxidativestressandtoinvestigatetheprotectionofAA213GMethods：Culturednorma

2、lrabbits’lensesinvitrowererandomlydividedinto4groups：controlgroup(exclusiveofH202)，H202group(involvinglmmoFL)，H202addedVcgroupandH202addedAA一2pGgroupThetransparencyofalllensesWaSdetectedafterabovelenseswhichwereincubated

3、for24tl’48hand72h；Attheendoftheincubationperiod，thelenseswereexaminedforbiochemicalparameterssuchassuperoxidedismutase(SOD)，glutathione(GSH)totalantioxidiationability(TAOC)andmalondialdehyde(MDA)byspectrophotometricallyA

4、tthesametime，thehistologicalchangesofalllenseswhichwereincubatedfor72hwereexaminedbyelectronmicrosopiesResults：1ThelongertimeincubatedintheRPMI1640exclusiveofH202，theopacityofthecontrolgroupWaSnotapparent；Thelongertimein

5、cubatewithH202treatment，themoreopaqueoflenswasfound；Thelensopaciticationwerefoundat12haftertreatmentwithH202，andthelensbecamealmostcompletelyopaqueinabout72haftertreatmentwithH202；TheopacityoftheVcgroupandAA2pGgroupwasap

6、parentlylowerthantheH202group2Tocomparewiththecontrolgroup，thelevelofTAOC，GSHandtheactivityofSODofthegrouptreatedbyhydrogenperoxidereducedstatistically；thelevelofMDAincreasedstatisticallyThelevelofMDAWaSsignificantlydecr

7、easedbutthelevelsofotherindexeswereincreasedinVcgroupandAA213Ggroupcomparedwiththoseoftheminhydrogenperoxidegroup3ThestructureoflensofthegrouptreatedbyhydrogenperoxideandtheVcandthegrouptreatedbyhydrogenperoxideandAA一2陽g

8、rouphadsomedamageaScomparedwiththecontrolgroupConclusion：iAA213Gcandelaythelensopacificationandmaintainthemdiaphaneity2ThelevelofSOD，GSHand％AOCwereincreased，butMDAdegradedAA218Gcaninhibitobviouslylipidperoxidationactivit