羧甲基殼多糖對(duì)皮膚成纖維細(xì)胞生長(zhǎng)的影響及生物組織相容性研究.pdf

1、青島海洋大學(xué)碩士學(xué)位論文羧甲基殼多糖對(duì)皮膚成纖維細(xì)胞生長(zhǎng)的影響及生物組織相容性研究姓名：王真申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：海洋生物指導(dǎo)教師：陳西廣2000.6.1童璺塑堂奎蘭雯主堂墊望蘭塑里苧查墨壁墅鏖壁盛塹絲塑墮竺蘭塑墅墮璺生塑望塑塑查壁塑!簍一TheeffectofcarboxymethylchitosanonthegrowthofskinfibroblastsanditsbioIogicalcompatibiIit)rCarboxyme

2、tllylchitosan(CM—CH)isawate卜solublederiVatiVeofchitos舡1Usingmeskintissueofyellowpeople，wesuccessfullyculturedfibroblastsofnormaladultskin，fbtalskinandkeloidinvitrDandwestudiedmeefrectofCMCHonmeproliferationof也esefibrobla

3、Stsandc01lagenmetabolismTheresultsdemons仃ated協(xié)atCM—CHpromotedproliferationofnolmalskinfibroblastsandmarkedlyinhibitedthegrowChofkeloidderivedflbroblastsCMCHmarkedlyreducedt11esynmesisofcollagenofkeloidfibroblastsTheresul

4、tsofmrtherstudydemonstratedCMCHirIlibitedthesyntIlesisoftypeIcollagenaIlddidnotch鋤gemesynmesisoftypeIIIcollagenforkeloidfibmblastCM—CHdidnotmarkedlyaffectthesynthesisofcollageninno哪alskin助roblastsCM—CHmembranewasprepared

5、toinVestigatetheaffinitybetweenCM—CHaJldserumproteinsTheresultssuggestedtllatCM—CHfilmcouldremarkablyadsorbserumpmteinsoncertainpHconditionsandtheadso印tionhadselectiVityFibroblastscouldadhereont11efilmofCM—CHandprolifera

6、tequicklyforalongtimeTheseresultsshowedCMCHhadgoodbiologicalcompatibili妙HemolysisandcelltoxicityexperimentsshowedthatCM—CHdidnotexpresstoxicityt0cellsKeywords：carboxymethyl—chitosall(CM—CH)肋roblastkeloidproliferationcoll