YN株鴨肝炎病毒的分離鑒定及表達(dá)YN株VP1基因的重組鴨瘟病毒構(gòu)建.pdf

1、ClassifiedIndex:U.D.C:DissertationfortheMasterDegreeinAgricultureYNSTRAINSOFDUCKHE刊汀ITISVIRUSISOLATIONANDIDENTIFICATIONANDRECONSTRUCTIONOFTHERECOMBINANTDUCKPLAGUEVIRUSWHICHEXPRESSEDTHEVPIGENEOFYNSTRAINCandidate:SuPerviso

2、r:Aeade而eDegreeAPPlledfor:SPeeiality:DateofOralExamination:University:ZhangHan洲anProf.HuangBaoxuMasterofAgriculturePreventiveVeterinaryMedicineJune，2010NortheastAgriculturalUniversity‘東北農(nóng)業(yè)大學(xué)農(nóng)學(xué)碩士學(xué)位論文4.4病毒感染力滴定......……，...

3、............................................................................................……164.5pTCL一VPI酶切鑒定結(jié)果...............................................................................................……174.64.74.

4、8重組病毒基因組PCR鑒定.............……，.，......................................................................……17重組藍(lán)白斑結(jié)果.........................................……，............................................................……18

5、重組病毒毒力滴定........................................................................……，.........................……184.9重組病毒接種刺激鴨瘟、鴨肝炎抗體水平測(cè)定........................................................……195討論......................

6、.................................................................................……，..…，..................……，.215.1病毒載體的應(yīng)用.............................……，..................................................................

7、......……215.2鴨瘟抗體檢測(cè)方法....……，.............................................................……，.........................……215.3重組病毒免疫后中和抗體檢測(cè)結(jié)果的分析................................................................……226結(jié)論.......

8、.......................................……，...................……，.................……，.................……，.........……23致謝.....................................................................................................

9、...................................……24參考文獻(xiàn)..................................……，...............……，.....................................................................……25附錄......................................……，......