醒腦增智方對AD模型大鼠淀粉樣蛋白前體裂解酶基因表達的影響.pdf

1、陜西中醫(yī)學院碩士學位論文醒腦增智方對AD模型大鼠淀粉樣蛋白前體裂解酶基因表達的影響姓名：彭紅春申請學位級別：碩士專業(yè)：中醫(yī)臨床基礎指導教師：焦俊英20100401XingnaobytheChileansideoftheADmodelratsamyloidprecursorproteincleavingenzymegeneexpressionAbstractPurpose：Thisexperimentaimsatstudyingtreat

2、menttraditionalChinesemedicineXingnaobytheChileansideoftheratswithAlzheimersdiseaseamyloidprotein(amyloidproteinA)precurscleavageenzymegeneexpressionindertoachievetheamyloidprecursproteincleavageenzymeactivityinterventio

3、n.totakeastepfwardtoexpletheprotectiveeffectofagentsonneuronsinthemolecularmechanismfthepreventiontreatmentofseniledementiaoftreatmenttraditionalChinesemedicinetoprovidenewideas.Methods：In80healthymalefemaleSDratsareeven

4、lydividedromlyed10astheblankcontrolgroup(nmalcontrolgroup).Theremaining70ratsareinjectedintraperitoneallyDgalactose6weeksafterthebilateralbasalfebraininratbrainnuclearinjectionofMeynertibotenicacid(IBO).2weeksaftertherat

5、ssurvivemodelingwatermazetestfilteroutthememylearningdysfunctioninratsasasignificantnextstepintestobjects.Pathologyofthesemiceareromlydividedintomodelcontrolgroup(modelgroup)thechinesemedicineXingnaobytheChileansidetreat

6、mentgroup(referredtoastraditionalChinesemedicinetreatmentgroup)brainrehabilitationtreatmentgroup(treatmentgroup).ThemodelgroupofratsfedwithphysiologicalwatertheChinesemedicinetreatmentgroupofratsgiven8.3gkgdWesternmedici

7、netreatmentgroupgiven500mgkgd2timesadayatotalof10weekseachgroupinthelastadministration2hourslaterdecapitatedhippocampustakentocarryoutHEstaininglightmicroscopeviewofinvestigationstheuseofreversetranionpolymerasechainreac

8、tion(ReversTranionPolymeraseChainReactionRTPCR)detectionofAPPcleavageenzymemRNA.Results：After10weeksoftreatmentofthechinesemedicineXingnaobytheChileansidethewatermazeexercisetimeexercisedistancethequadrantofthememyfreque