微波固態(tài)合成低聚糖及其對小鼠抗氧化和免疫活性研究.pdf

1、江南大學(xué)博士學(xué)位論文微波固態(tài)合成低聚糖及其對小鼠抗氧化和免疫活性研究姓名：李新明申請學(xué)位級(jí)別：博士專業(yè)：食品營養(yǎng)與安全指導(dǎo)教師：樂國偉20080601江南大學(xué)博士學(xué)位論文AbstractMicro眥Ive嬲sistedsolidstales)，Iltllesisofoligosacch耐deso虢rSusaIlidealresol“on塒mtlleadV觚tagesofr印idi劬lli曲yielda11dlessenv的mentalp

2、ollutionIIlⅡlepresentwork，weoptimizedtllereactionconditionsofmicrowave—assistedsolidphasesynmesisofoligosacch撕des(gluCooligosaccharides(GOS)鋤dm鋤00ligosacch撕des(MOS))Andmee岱：ctsofMOSontllescaVengingrateof丘eemdic甜s，t11eant

3、ioxidaIltenzymes觚dimmlmjt)ractiVitiesiIlmicewereiIlVestigatedOptimalexperiInentshowtllattheoptimalconditionof面crowaVeassistedsolidphaSesynthesisofGOS嬲fono、髑：1000w0fmicrowavepow％9millofmicrowaveinadiationtime，30％water嬲ini

4、tiator，4％phosphoricaCid嬲tllecatalystTheyieldofproductw嬲7656％Theproduct、ⅣascharaCterizedbyHPLCThecomponentsofsyntheticproductwaSdetemined嬲follows：2081％disacch撕des，1550％trisacch撕des，1084％t酏滔aCcharides，779％pentsaCch撕des，216

5、3％01igosacch撕deswim11ighermoleculeweightbeyondhexsaccharidesTheoptimalconditionofIIlicrowa、，e2LssistedsolidphaSesyntbesisofMOS嬲f01lo、ⅣS：1000wofmicrowavepower，4miIlofmicrowaveirradiationtime，15％water嬲i11itiator，3％phosphor

6、icacidaSt11ecatalyStTheyieldofproductw嬲8650％Theproductwascharact耐zedbyHPLCThecomponentsofsyntlleticproductwasdete冊iIled硒foUows：1350％mallIlosaCcharides，382％disaCch撕des，756％trisacch撕des，684％tetrsaCcharides477％pemsaccharide

7、s，554％hexsacch撕des，8650％謝t1111i幽ermolecule、ⅣeightbeyondheptasaccharideTheresultsi11dicatetllatMoScouldemciennyscavengetllehydroxylradicalinvitro謝mtlleIC50of4m∥m1The撕malexperimentshowthatm曲fatdietordgaladministlationcaIls

8、igni矗calltlyincreaSedoxidativestress(尸O05)comparedtotheyoungconn011kMOSdietadministrationcaIlsi鰣ficantlyincreasedtheWei曲tofth)恤us，spleen，liVeraIldkidneyandthelevelsofsemmlgGLgAa11dlgM(尸005)Moreover，allmicefeeded謝tllMOSdi

9、etshowasigllificantincreaseinthestimulationindeX(SI)(witll／withoutCorA)oft：hyInusaIldspleen妒o05)ItismereforebelievedtllattheMoSdietcanenhancetlleactivityofantioxidalltenzymesandimproveimmuIlityactivi鑼inmiceKeywords：Gluco