C-myc反義核酸對肝癌細胞增殖、凋亡、細胞周期的影響.pdf

1、蘭州大學碩士學位論文Cmyc反義核酸對肝癌細胞增殖、凋亡、細胞周期的影響姓名：李振軍申請學位級別：碩士專業(yè)：外科學（普通外科學）指導(dǎo)教師：郭天康李一平20060501——～蘭型查蘭墮塞生鱟焦造壅AbstractObjectivesToinvestigatetheeffectofCmyeantisenseoligo‘deoxynucleotide(ASODN)Oilgrowth，apoptosisandcellcycleofHEPG2ce

2、llsMethodsHEPG2cellswereculturedconventionallyGrowthinhibitionrateofHEPG2cells，afterhavingbeentreatedwithCmycASODN，WeredetectedbyMTTmethodMorphologicalchangeofcellswereobservedunderafluorescentmicroscopeDNAladderwasexami

3、nedbyDNAelectrophoresisTheapoptoticmteandcellcyclephasedistributionwereexaminedbyflowcytometeranalysisTheexpressionofCmycgenewasdetectedbyimmunohistochemislrystainingandRTPCRonthemRNAorproteinlevelrespectivelyResultsTher

4、esultofimmunohistochemicalandRTPCRassaysshowedthatC—mycASODNcoulddecreasetheexpressionofC—mycgene。ThegrowthinhibitionofHEPG2cellsafterhavingbeentreatedwithCmycASODN’wascertainedbyMTranalysis。Furthermorewiththeconcentrati

5、onincreasingofC，mycASODNandtimepassing，theinhibitionabilityalsoelevatedCellapoptosiswasidentifiedbyfluorescentstaining，DNAeleclrophoresisandflowcytometerAtthesametime，CmycASODNcouldblockcellscycleonGa／G1phasebyflowcytome

6、teranalysisConclusions圓emycASODNcouldinhibitthegrowthofHEPG2cells。⑦CmycASODNcouldinduceapoptosisofHEPG2cells@C—mycASODNcouldblockcellscycleonGo，GlphaseKeywordsCmycanfisenseoligodeoxynucleotidehepatoceltularcarcinomacellI