牛乳中抗菌類藥物、雌激素殘留檢測(cè)及其營(yíng)養(yǎng)素含量的研究.pdf

1、甘肅農(nóng)業(yè)大學(xué)碩士學(xué)位論文牛乳中抗菌類藥物、雌激素殘留檢測(cè)及其營(yíng)養(yǎng)素含量的研究姓名：趙金蓮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：孫曉林許敬東20080601甘肅農(nóng)業(yè)大學(xué)碩士研究生學(xué)位論文SUMMARYSUMMARYToinvestigatethephysicalpropertiesof舶shIIlill【，theresidueofantibioticsandhormonein510freshmilksamplesofdairycat

2、tlefromfarmeroffivecities(Lanzhou，Tianshui，Hezuo，WuweiandLinxia)inGansuprovince，itwasdeterminatedthatthephysicalpropefliesoffreshmilk，water，protein，fat，nutrientcontentandthatothermajorelementswithGBmethodInaddition，itwas

3、dectectedthattheaminoglycosides，sulfa，tetracyclineclass，quinolones，andotherbroadcategoriesofantibioticsbyusingmicrobioanalysis，high—performanceliquidchromatographyandkitBesidesthedectectingmethodofthreekindsofestrogenous

4、residues(estradiol，diethylstilbestrolandestrone)inmilkwastobefoundbyhighperformanceliquidchromatographyTheresultswereasfollows：(1)Thecolorofmilkwas110abnormal，andthetissueofmilkwasaslightviscousphenomenoninthefivecities；

5、therelativedensityof12sampleswerelowerthanthenationalstandardinWuweicity50samples喇thwater，protein，fatandnutrientcontentandothermajorelementsweredeterminedinfivecities，whichwe豫foundthattheproteincontentwaslowerthanthenati

6、onalstandardofsamples，4inIABzhoucityand5Tianshuicity；Thecontentoffatwaslowerthanthesampleofnationalstandards，3samplesinLanzhoucityandfivesamplesinWuweicity(力TheresidueofantibioticsinmilkWasdeterminedby11rCmthodandpaperme

7、thod，whichshowedtheaveragepositiveratewas94％TheminmumdetectionlimitofsulfanilamidegroupandtetracyclineantibioticsWas03mg／kg，05mg／kgrespectivelyandthecontentswerebetween030and125mg／L；positiveratewas107％，8O％respectivelyStr

8、eptomycin，Quinolone，ChlormycetinandsulfamethazineWasdetectedrespectivelybykitmethod，whichshowedaveragepositiveratewas117％、36％、78％and9％(3)Theefficientmethodforsimultaneousdeterminingthreeestrogens(estradiol，diethylstilbes

9、trolandnorethisterone)inmilkbyhighperformanceliquidchromatographywasdevelopedThesampleWasdealedwithacetonltrfletemovetheproteinprecipitationsupernatantenrichmentAcetonitrilewater(volumeratio，1：1)Wasusedforthemobilephasef

10、oranalysisThreekindsofestrogenWereseparatedwellTheaveragerecoveryrateWasbetween816％and958％TherelativestandarddeviationWasbetween15％and42％ItshowedthattheminimumdetectionlimitWasat10—20ng／mLKeywords：milk，antibiotics，sulfan