馬巴貝斯蟲Be82基因片段的克隆、表達及其在診斷中應用的研究.pdf

1、中國農(nóng)業(yè)大學碩士學位論文馬巴貝斯蟲Be82基因片段的克隆、表達及其在診斷中應用的研究姓名：王玉玲申請學位級別：碩士專業(yè)：預防獸醫(yī)學指導教師：趙德明20060901中國農(nóng)業(yè)大學碩卜學位論文AbstractAbstractBabesiaequiisabloodparasitewhichcauseshorsesahemoprotozoandiseasefrequentlycalledasEquinebabesiosisEquinebabesi

2、osisisaseriousdiseaseandhardtobeerasedIthasaconsiderableimpactoninternationalhorsetradeSoitisaimportanthomediseasesinspectedbycountriesSensitiveandspecificserologicaldiagnosisisamajorsubjectresearchedbyscientistsincountr

3、iesInthisstudyBc82AgeueofBequiissyneticedrecombinedexpressioncarriersoftwoBe82geuefragmentsandonccompleteBe82geueareconstructedbyPCRamplificationenzymescoring，ligation，andsuccessfullyexpressedinthefusionexpression∞lTiero

4、fpGEX4T1ByPCRthelengthofthethreeexpressedBe82genefragmentsis192bp306bpand420bptheresultsamconfidentwithexpeoedBySDSPAGE，theresultshowsthatthesizesofthethreeproteinsexpressedis33KD37KDand42KDByELlSAallofthethreefusionexpr

5、essedproteinsgiveaspecificreactionwithBequlcontrolpositiveserumtheyshowthatthesethreeexpreesionproteinshaveaprotencyasdiagnosisantigenELISAbasedoffthesethreeantigensrespectivelyistestedinsmallsamples，theresultssuggesttha

6、tsensitivityof33KDproteinishigherthan37KDand42KDandthen33KDproteinexpressedisconfirmedasdiagnosisantigentodetectBequLExporthorseseraarctestedbyELISAestablishedinthisstudyandbytraditionaltestofcomplementfixationtesttheres

7、ultsindictethattheconfidencerateofthesetwotestis100％ThesesuggestthatELISAestablishedinthisstudyhaveapossibilityinplaceofcomplementfixationtestAllaboutBe82genefragmentissuccessfullyconstmcedandexpressedinthisstudysensitiv

8、eandspecificBequldiagnosticantigenisscreenedfirstlyinom“countryELISAbasedOntheantigencouldbeusedtodetectBequlpositiveanimalsprovidestechniquetoinspectentryexithorses，solvesthedifficultquestionthereistoBequidiagnosticreag

9、entsinonrcountrymakesaavailabilitytoproduceBequidiagnosticreagentsItisusefulindiagnosing，quarantiningcontrollingandclearingBequiItprovidesamethodfursupervisingandinvestigatingBequiKeyWords：BabesiaequiBe82gene，expression，