水蛭滲濾液對(duì)凝血酶誘導(dǎo)的人視網(wǎng)膜色素上皮細(xì)胞周期的影響.pdf

1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文水蛭滲濾液對(duì)凝血酶誘導(dǎo)的人視網(wǎng)膜色素上皮細(xì)胞周期的影響姓名：左玉霞申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中醫(yī)眼科學(xué)指導(dǎo)教師：鄭燕林20060401成都中醫(yī)藥天學(xué)碩：t學(xué)位論文AbstractPurposeToinvestigatetheeffectsofleechpercolateOncelldivisioncycleofculturedhumanretinalpigmentepithelium(hRPE)cellsindu

2、cedbythrombinExploringmechanismofleechpercolateonpreventingandcuringPVRMethods(1)Humanretinalpigmentepithelium(hRPE)cellswerecultured／nv／trowithdigestculturemethod。(2)Theselectedconcentrationsofleechpercolatef128mg／ml、64

3、mg／ml、32mg／ml、16mg／mtand8mg／mI)werebasedonthepreviousexperimentsWithorwithout0SNIHU／mlthrombin，hRPEcellsweretreatedwithleechpercolatebyvariousconcentrationsfor24hrsandtheeffectsofcelldivisioncycleofleechpercolatewerestud

4、iedwithFlowCytometer(FCM)。(3)WithorwithoutO5N／HU／mlthrombin，hRPEcellsweretreatedwith64mg／mlleechpercolateatdifferenttimes(6hr、12hr、24hrand48hr)，andtheeffectsofcelldivisioncycleofleechpercolateweretreatedwithFCMResults(t)

5、TherewasageneraltendencythatleechpercolateoftheselectedconcentrationsarrestedhRPEjceilsonGo／Glphaseand64mg／mlleechpercolateinhibited779％hRPEcellsOn氌鼯l癆aseandmadehRPEcellsoRSphaselessespecially(2)Duringtheperiodof6hrsand4

6、8hrsthelongerthetreattimeof64mg／mlleechpercolate，themoretheRPEcellsarrestedonGo／GlphaseConclusionTheresultsapproveleechpercolatecallinhibitproliferationofhumanRPEcellscultured跏vitroItsmechanismmaybethatleechpercolatecoul