急性白血病MLL基因重排的檢測.pdf

1、中國協(xié)和醫(yī)科大學碩士學位論文急性白血病MLL基因重排的檢測姓名：趙佳煒申請學位級別：碩士專業(yè)：病理學和病理生理學指導教師：王敏20070501中國醫(yī)學科學院中國協(xié)和醫(yī)科大學碩士學位論文DetectionofMLLGeneRearrangementsinPatientswithAcuteLeukemiaAbstractRearrangementsofMLLgeneoftenOCCUTFcdinacuteleukemiaespecially

2、treatedwiththeinhibitorsofmpolIMLLgenelocatedat11q23anditisdifficultbyconventionalcytogeneticanalysistouncovertheabnormal1lq23SoisFISHmethod，whichistimeandlaborgOllSUnledandhardtodetecttheminimalresidualleukemiccellswith

3、abnormal1lq23ThusweplantosetupaconvenientandrapidmethodtodetectMLLgenearrangementsinpatientswithleukemiaThenthedetectionresultinturnwouldcontributetotheclinicaldiagnosisofleukemiaandtherapy—relatedMLLgenearrangementsWees

4、tablishedtwotypesofpanhandlePCRmethods，panhandlePCRandreversepanhandlePCRtodiagnosetherearrangementsofMLLgenePanhandlePCRandreversepanhandlePCRmethodsareobviouslybetterthantheconventionaldetectionmethods，such船cytogenetic

5、analysis，F(xiàn)ISHexperiments，andspecificRT—PCRmethodsTheyarebothuniveralmethodswithhighsensitivity，easytoapplication，andtheyrequireonlysmallquantitiesofgenomicDNAAndwhat’smorebothofthemcouldidentifyeachchromosomalbreakpoints

6、itesandobtainmoredetailedinformationThenthoseinformationcouldcontributetofurtherunderstandthemolecularmechanismofMLLarrangementsandtherelationshipamongthesubtypeofMLLarrangements，therapeuticregimens，andclinicalprognosis1

7、11eacuteleukemiapatientstreatedwiththetopolIinhibitotsandthedenovoacuteleukemiapatientswereusedinourstudy，andthepatientwitht(4；11)aspositivecontr01WesuccessfullyampplifiedtheabnormalbandbypanhandlePCRandreversepanhandleP