HAP納米粒子對肝癌細胞及其端粒酶基因表達的影響.pdf

1、武漢理工大學碩士學位論文HAP納米粒子對肝癌細胞及其端粒酶基因表達的影響姓名：王紅暉申請學位級別：碩士專業(yè)：生物醫(yī)學工程指導教師：閆玉華2003.5.1武漢理工大學碩士學位論文目@!!㈣e!_目!_≈!!≈E!_!!女e!s_!g_目!_!!se!_∞!!㈣!ABSTRACTTheeffectofapatitenanoparticlesoncancercellsisakindofbiologicaleffectofinorganicna

2、noparticles，andithadalsobeenstudiedbytheBiomedicalMaterialsandEngineeringResearchCenterofWUTsincemanyyearsbefore。Onthebasisoftheformertesearchtheauthorselected‘TheeffectoflAPnanoparticlesonthehepatocellularcarcinomacella

3、nditstelomerasegeneexpression’ashismainresearchcontentinthesubjectBystudyingthetelomeraseactivity，thepurposeofthisarticleistoinvestigatetheanti—carcinomaeffectandmechanismofapatitenanoparticlesattheviewpointofgenelevel，a

4、ndfinallytooffersometheorygroundsforbasicandclinicalmedicineFortheexperimentstheBel一7402andL—02wereculturedinvitroWiththehelpofcomputerimageanalysesystem，theircharacteristicsandbiologicalbehaviorswereobservedTheMethylthi

5、azolyltetrazolium(MTT)testusuallyusedtoevaluatetheproliferationofcellswhichculturedinvitrobecauseofltssuchadvantagesashighsensitivity，goodrepetition，fastandsimpleoperation，andlittleinfluencebySUbjectivefactorsExtracorpor

6、ealcellcultureandMTTcolormetricassaywereusedtomeasuratethechemosenstivityofhepatomacellstotheHAPnanoparticlesandfivekindsoftraditionalantitumordrugs，suchascisplatin，doxorubicin，vincristine，mitomycin，cyclophosphamideInord

7、ertOdeterminetheeffectofHAPnanoparticlesontheexpressionoftelomerasegene，thetelomeraseinsituhybridizationtestkitwasusedandtheresultwascontrastwiththatofatelomeraseinhibitor(eisplatin)Then，conclusionsaremade：1Inthesameoper