粘著斑激酶和PCNA反義寡核苷酸治療胃癌的研究.pdf

1、Y950025分類號(hào)；R_7352學(xué)校代碼l10392學(xué)號(hào)：2003255福建醫(yī)科大學(xué)碩士研究生畢業(yè)論文粘著斑激酶和PCNA反義寡核苷酸治療胃癌的研究TheeffectiononStomachCarcinomaCellbyAntJsemseOllgonucleotldmTargetingtheMexseagerRNAEncodingFoealAdhesionKiBiiileandPro|ireratingCellNuclearAntig

2、en所在院(系)：福建醫(yī)科大學(xué)協(xié)和臨床學(xué)院研究生：江紅星學(xué)科(專業(yè))：腫瘤學(xué)導(dǎo)師：官國先副教授黑心愿教授研究起止日期，2005年3月至2006年3月二霉零六年三月福建醫(yī)科大學(xué)碩士學(xué)位論文TheeffectiononStomachCarcinomaCellbyAntisenseOligonucleotidesTargetingtheMessengerRNAEncodingFocalAdhesionKinaseandProliferatin

3、gCellNuclearAntigenAbstractobjeetivesToinvestigatetheeffectsofFAK、PCNAantisenseoligonucleotidesonthehumanstomachcarcinomacellsviasynthesizingFAK、PCNAantiscnseoligonuclcotidestoworkOilhumanstomachcarcinomacells—SGC7901Met

4、hodsTheantisenseoligonucleotideswb∞r(nóng)esynthesizedcomplementarytotheproteinencodingreg“onsofFAKmRNAandPCNAmRNAandintroducedintothehumanstomachcarcinomacellsSGC7901respectivelyOrassoeiatelyTheeffectionsWb豇eestimatedbycellco

5、lonyforming、cellgrowthcurves、cellimmunoehemistrystaimgmethod、nn，e讎transcriptionPolymaraseChainReaction(RTPCR)andTunnelMethodResultsThecellproliferationwasinhibitedeffectivelybyantisenseoligonucleotides，thecolonyformation

6、ratedescendsharplytheinhibitioneffectstaperedafter72hours，theexpressionsofFAKmRNA、PCNAmRNAandtheirproteinalsodecreasedsharplyandthecharacteristicapoptosisWasinducedConclusionTheFAK、PCNAantiscnseoligonucleotidesCaninhibit

7、sthehumanstomachcarcinomacells’proliferationandtheexpressionsofFAKmRNA、PCNAmRNAandtheirproteineffectivelyTheantisenseoligonucleotidesCaninducethehumanstomachcarcinomacellstoapoptosisKeywordsFAKPCNAantisenseoligonucleotid