18804.134株克羅諾桿菌的多位點序列分型研究

1、南開大學(xué)碩士學(xué)位論文134株克羅諾桿菌的多位點序列分型研究姓名：李昆申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：王磊201205AbstractAbstractThegenusCronobaeteriscomposedofGramnegative，facultativeanaerobicrods，whicharemembersoftheEnterobacteriaceaeFamilyItwasoriginallydefinedasaspe

2、cies(Enterobactersakazatai)andincluded16differentbiogroupsRecently，CronobacterhasbeenreclassifiedasagenusTheprimaryreservoirofCronobacterspphasnotbeendetermined，butitispostulatedthatplantmaterialmaybeanimportantsourceCro

3、nobactersppstrainshavebeenisolatedfromawidevarietyoffoodsandwater，includingmilk，cheeseandpowderedinfantformula(PIF)SurveillancestudieshavedetectedCronobactersppinhouseholdsandfoodfactoryproductionfacilitiesCronobacterspe

4、ciesareopportunisticpathogens，maycauseinfectionsacrossallagegroupsHoweverneonates，particularlythoseoflowbirthweight，arethemajoridentifiedgroupatriskwithahighmortalityrateTheorganismisacauseofneonatalmeningitis，necrotisin

5、genterocolitis(NEC)andsepsisAnumberofoutbreaksofCronobacterspphavebeenreportedinneonatalintensivecareunitsaroundtheworldPowderedinfantformulahasbeenidentifiedasamajorsourceoftheseoutbreaksRecentreportshavehighlightedther

6、iskposedtoimmunocompromisedadultswithbacteremiaandosteomyelitisThemultilocussequencetyping(MLST)schemeWasdesignedtoprovideatoolforinvestigatingpopulationsandpopulationdynamicsonaglobalscaleItappliesneutralorslowlyaccumul

7、atinggeneticvariationinhousekeepinggenesTheevolutionofthesehousekeepinggenesisconstrainedbytheirrequirementtoencodefunctionalproductsandisnotaffectedbytherapidevolutionthatmaybedetectedwithingenesencodingproteinsthatinfl

8、uencesurvivalinaparticularnicheMLSTalsesolvesothermoleculartypingtechniques’shortfalls，whicharethattheirresultscouldnotbecomparedamongdifferentlabsInthisstudytheMLSTschemehasbeenusedforthefastandreliableidentificationand