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1、浙江大學(xué)碩士學(xué)位論文大麻素受體對(duì)神經(jīng)干細(xì)胞分化后神經(jīng)突起生長的影響及其信號(hào)轉(zhuǎn)導(dǎo)機(jī)制姓名：鐘婷婷申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：孫益201009浙江大學(xué)碩士學(xué)位論文EffectsandsignaltransductionmechanismofcannabinoidreceptorsonneuriteoutgrowthofdifferentiatedneuralstemcellsAbstractWefocusedour
2、studyontheeffectsofcannabinoidreceptoronneuriteoutgrowthofneuronscomingfrommouseneuralstemcellinvitroandthepossiblesignaltransductionmechanismWefirstlyculturedneuralstemcells(NSCs、insuspensioninserumfreemediumcontainingb
3、asicflbroblastgrowthfactor(bFGF)andepidermalgrowthfactor(EGF)111eNSCsarefloatedasmulti—cellaggregatescalledneurospheresinvitronleneurosphereswhicharenestinpositiveCanbepassagedandCandifferentiatetoneuronsandgliacellsInor
4、dertostudytheeffectsofcananbinoidreceptorsonneuriteoutgrowth，weinducedtheNSCstodifferentiatemostlyintoneUmnSTheendogenouscannabinoidsystemhasbeenfoundwidelyexpressedinvarioustissuesandorgansofmammalianTodate，twomajorcann
5、abinoidreceptorshavebeendescribed，CB1receptors(CB1R)andCB2receptors(CB2R)CB1RispresentmainlyinCNSandmodulatekindsofneurophysiologyfunctionsTheCB2RisthoughttobeexpressedonlyinperipheralsystemHoweverithasbeenreportedrecent
6、lythatCB2RisalsolocalizedindifferentcellpopulationsinCNSTodoSO，weinvestigatedthepresenceoftheCBlRandCB2RmRNAsintheneurons，astrocytes，NSCsanddifferentiatedcellsfromNSCsbyRT—PCRWefoundthatCB1RandCB2RmRNAswereexpressedbya11
7、thekindsofthesecellsSubsequently，WestudiedtheeffectsofWIN552122(WIN)，amixedCB1R／CB2Ragonist，onneuriteoutgrowthofdifferentiatedcellsfromNSCsinvitroThroughtheneuriteoutgrowthassay，WINhadbeenshowedtoimproveneuriteoutgrowthi
8、naconcentration—dependentmannerTobettercharacterizetheinvolvementofeachsingleCBreceptorinWINenhancedneuriteoutgrowth，wecoincubatedthedifferentiatedcellswithWINandeithertheCB1RantagonistSRl41716AortheCB2RantagonistAM630As
9、aresult，botllofSRl41716AandAM630wereabletosignificantlydecreasetheaveragelengthofneuritetreatedwithWINItsuggustedthatWINenhancedtheneuriteoutgrowthbyactivatingbothCB1RandCB2RTodelineatehowWINenhancedneudteoutgrowthweexam
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