29161.重金屬cd納米抗體的制備及其免疫學(xué)檢測方法的建立與應(yīng)用

1、ShanghaiNormalUniVersityMasterDissertationDeVelopmentandApplicationofanImmunoassayforDetectionofCdBasedonSpecificNanobodyAgainstCd—ChelateDep扒mentSpecialt)，Life鋤dEnvimnmentScienceCollegeMicrobiologyResearchDirectionMicro

2、biology&FoodNameJiangR礎(chǔ)嘴nSupervisorZhouXiaoliCompleteDataMarch2014人BSTRACT卜海師范大學(xué)碩士學(xué)位論文ABSTRACTWiththerapiddeVelopmemofglobalindustIy，thepresentsituationofheaVymetalspollutionofourcountryaJldeVentheworldbecomesmoreandmore

3、serious，thishasarousedthegreata吐entionofscientistsaroundtheworld，theproblemisimminentCurrentanalyticalmethodsfordetectionofheaVymetalsincludemicr0spectralanalysis(MS)，ultmVioletVisiblespectrophotome仃y(UV)，atomicsbso叩tion

4、spectrometry(AAS)，atomicnuorescencespectrometry(AFS)，Xnuorescencespectroscopy(XRF)，andinductiVeIycoupledplasmamassspectrometry(ICPMS)，etcwhicharesensitiVea11dreliableHoweVer，thesemetllodsareexpensiVeandrequirehighlyspeci

5、alizedappamtusajldtrainedoperators，time—consumingandcostlytodetect，whichma：kesthemunsuitdbleforonsite(1etectionBasedontheSeconsiderations，thebuildofasimple，r印id，specificandsensitiVedetectionmethodhasbecomeahottopicImmuno

6、logicaldetectionmethodsforheaVymetaldetectionproVidesanewstrategyandapproach，whichisahi曲1yspecific鋤dsensitiveanalytica】methods，andthesimple，inexpensiVe，shortdetectiontime(approximately3～5min)，suitableforlargescalescreeni

7、ngsiteAtpresent，domesticimmunoassayforheaVymetalscadmiumfocusedoncadmium(Cd)，lead(Pb)，mercuD，(Hg)，cl啪mium(Cr)ando血erheaVymetalwimhightoxicityHereweselecttheheavymetalcadmium(Cd)for廿1estudy，usingPhageNaJlobodydisplaytecll

8、llologysuccessmllydemons仃atedanoVelteclllliquespecificforCdDTPAnon—traditiO僦monoclonalamibodyN鋤obody0妯)，initiallyestabliShedtheindirectcompetitiveELISAimmunoassays，鋤dfortlledetectionofcadmiu】!11ioninwaters鋤plesInt11isStu

9、dy，p—SCN—BnDTPAwaschosenasabi觚“onalchelatortofacilitatethesynthesisofantigensforCd，andgainedt11ecoInpleteantigeIlCd—DTPA—BS～OVAsuccess砌lyThephageaIltibodiesagainstCd—DTPA：CdDTPAVHH418aIldCdDTPAVHH615wereproducedthroughph

10、agedisplaytechn0109ysuccess向llyaIldCd—DTPA—VHH418isthebest，therelativeamnityis595108L／m01，thereisnocross—reaetivityeXistsbetweentheantibodya11d廿1ecarrierproteinBS～OVAaIldalsotllenonmetallicaIl_t培enDTPA—BS～OVA，a11dalmoStn

11、ocrossreactiOnwithothermetalionsTheinitiallyestablishedindirectcompetitiVeELISAimmunoassaymethoddetectionforthedetectionofCdwiththepreparedphageantibodywasmetthenationalstandardofwaterqualit)rmonitoring7I’helowestdetecti