30224.乙酰輔酶a羧化酶αct亞基acca基因表達(dá)載體的構(gòu)建及在杜氏藻中超量表達(dá)的研究

1、廈門大學(xué)碩士學(xué)位論文乙酰輔酶A羧化酶αCT亞基accA基因表達(dá)載體的構(gòu)建及在杜氏藻中超量表達(dá)的研究姓名：任妮申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：水生生物學(xué)指導(dǎo)教師：劉仁海劉廣發(fā)20090601AbstractItiseffectivetorelievethepressurefromconventionalenergyshortageandenvironmentandestablishthesustainableenergysystembyexplo

2、ringandusingbiomassTheexploitationandutilizationofbiomassenergyareeffectivestratagems，whichnotonlyrelievethepressurefromconventionalenergyshortageandenvironment，butalsoestablishthesustainableenergysystenLAmongnumerousorg

3、anisms，microalgaeareconsideredasexcellentcandidatesforbiomassenergyproducing，becauseoftheirlargerbiomass，fastergrowthrateandhigherpercentageof1ipidcomponentsInthispaper，Dunaliellaba_rdawiLflhalophilousalgea，wasusedasatra

4、nsgenticbioenergyproducerTheaccAgenewhichencodedQ—CTsubunitofacetylcoenzymeAcarboxylase(ACCase，akeyenzymeoflipidssynthesis)wastransformedintoitschromosomeItisexpectedthatalargenumberofmutatedaccAproteinwasexpressed，andth

5、ecapabilityofproducinglipidswasimprovedobviouslyInthiSstudy。theaccAgeneofACCaseenzymeofthe叵coliwascloned。theninvertedintoaprokaryoticexpressionplasmidPGEX一6p—laccAandthentherecombinedplasmidexpressedin￡colistrainResultss

6、howedthattheexpressedmutatedaccAproteinaccountedfor294％ofthebacteria’Stotalprotein，andmostofthemexistedasinsolubleproteinthroughSDS—PAGEanalysiSTheshuttlevectorpaccACATwithareportergeneCATandaresistancegeneofchlorampheni