帶魚下腳料蛋白水解螯合物制備及生物特性研究.pdf

1、中國海洋大學碩士學位論文帶魚下腳料蛋白水解螯合物制備及生物特性研究姓名：鐘明杰申請學位級別：碩士專業(yè)：生物工程指導教師：陳西廣20090603PreparationofcheIateofproteoIysisfrom局囟廠e力獸j“／a2鋤s，BIeeker’sIeftoveranditsbiologicaIcharacterstudyAbstractInthisp印er’theionsbindingconditionsofhydrol

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3、fsofcontentaSfollows：1Thee丘ectofhydr01yzedtimeonDHintheconditionsoff獅ordefat哆bycompounden巧mes2Theeffectofdif凳remDH，pHtemperatureandtimeonthechelateratioofFepandJ如彤餾甜么Zz們船≠Bleekerenzymatichydrolysate3Infraredspectrumanaly

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5、iIltheconditionsofdef；啊or2h，4h，10h，23hintheconditionsoff≥啊2)T11eoptimalchelatingFe什ratiowas94，15intheconditionofDH5％，pH7，temperacure20℃time30min3)ThepeptidescouIdchelatemetalionsandtheycombinedwithmonodentatecoValentbond

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7、ject，theantibacteriaIactivi哆ofpeptideschelatingFe2hadbeendeternlinedbythemethodofoxfordCupt、VoIayerpIatinginvitro，respectivelyAndinhibito彤e腩ctofthechelatin薩e什byfhctionaIprecipitationwith80％ethanolto召卯川搬s柏f池，麗印砂幻∞cc搬口班哪wa