簡介：新疆農(nóng)業(yè)大學(xué)碩士學(xué)位論文日糧中添加亞油酸和維生素A對乳腺組織和肌肉組織中硬脂酰輔酶A去飽和酶的影響姓名張瑞超申請學(xué)位級別碩士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師王加啟羅淑萍20060501ABSTRACTEXPEDMENTSWERECONDLLCTEDT0INVESTIGA把MEE任BCTSOF1DIETARYLIONLEICACIDLAONGENEEXPRCSSION趾DACTIVITYOFSTEAROYLCOADES咖RASESCDIN刪砌ARYGL鋤DOFLAC詛TMGGOAT；AILD2DIE卿VITAINILLAVAONSCDACTIV時(shí)OFLONGISSIMUSDORSIMLLSCLEINCROSSBIEDBEEFCATTLEINEXPERIMEM1，伽DVEXINONGS鋤ENGOATSDAYSILL“LL【AVEMGED36士3WEREMDOMLYASSIGINEDTO押MGROUPSN6、】VITLLACO玎叩LETERANDOIILIZEDDESIGND昭IGNTFE咖EN協(xié)CONSISTEDOFAOOR40眺GDMLASUPPLEMENT0NDAY28皿猢ARYGL趾DMOPSIESWEREPERF嘶NEDBYSURGERY姐DAPROTIONOFTHETISSUE托ALYZED如RMRNAE坤嘴SSIONOFSCDBYREALTIRNEPCR111EACTIV姆OFSCD、V夠EVALUATED、L，IⅡLAILOTLLCRPONIONOFMCDSSUECOMPAREDTOCONTROLGROUP0SUPPLEMENTOFLA，GOATSFED40舭GDMOFDIETARYLADECREASEDTLLESCDGCNEEXPRESSIONINM蝴ARY91AILDP005HOWEV％ITSHOWEDA舢NERICALDECRE船EOFSCDACTIVITY訪40艫徊MOFDIETARYLASUPPLEMENT111EXPERIMENT2，LI咖INN1【ICMSSBREDBEEFCATTLESO儼32，12MTLLOLD誦TLLAAVERAGEBODYWEIGHTOF350士5KG、VERE珊DOMLYALLOCATEDT0O∞OFFOLLRGROUPSC砌ESINFOUR仃齠衄即臨WE∞FEDDIETSCON劬LG0，50％，100％觚D200％OFME2200I唧MB鶴ALDIETARY、德，RESPECTIVELYTA11CAMESWEREWEIGHT酣MO餓111Y缸D3C枷ES、訂ILLINEACHGOILPWERESLAUMEREDAFTER3MONTLLSFEEDIRLG，LONGISSIMUSDO瑙IMUSDES蛐PLESWERCTA_KENFORDCTE珈【LIIL撕ONOF也ESCDACTIV如111ERESTC砌ESWERENOTSLAUT啪DUNTN6MON也OF觸MNG。D嘶NGME3MON也FEED啦，血CSCDAC廿VITYW鵲LLI曲ERILLVASUPPLEMENTGROUPSTLLAN也CCON廿OL掣OUPO05，W11ILEDIDNOTDI儆AMONG50％，100％AND200％SUPPLE砌ENTGR01JPS，O05HOWEVER，MESCDACLIVITYIIL刪SCLESOFC刪ESOF6IN伽瞳HFCEDINGDE鍶ASED勰DIETARYWLINCRE勰MGINLOO％AND200％GROUPS11LCONCLUSION，LACTATINGGOATSFEDWIM40眺GDMLADECREASEDMMAEXPRESSION趾DACDVITYOFSCD證MA【毗NARYGIANDT1IEAPPMPRIATEDOSEOFⅥ～COULD硫REASESCDACTI訴TYOFLON舀SSIMUSDORSIMUSCLEINASHONPERIODKEYWORDSLINOLEICACID，VIT毒IININA，STEAROYLCOADES柵E，GENECXPRESSIONII

簡介：中國農(nóng)業(yè)大學(xué)博士學(xué)位論文小鼠乳腺原基中干細(xì)胞定位及其再生功能性乳腺姓名韓建永申請學(xué)位級別博士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師李寧20060601～中國農(nóng)業(yè)大學(xué)博I學(xué)位論文ABSTRACTABSTRACTTHEMAMMARYGLANDHASBEENUSEDASANIDEALMODELFORTHERESEARCHESONDEVELOPMENTBECAUSEOFITSSPECIALTYOFSTRUCTUREANDDEVELOPMENTDURINGTHEBREASTMORPHOGENESISANDDEVELOPMENTTHEPROLIFERATIONANDINVOLUTIONOFMAMMARYGLANDINDIFFERENTSTAGESAREDUETOTHESELFRENEWAL，PROLIFERATIONANDDIFFERENTIATIONOFMAMMARYSTEMCELLSTHELOCALIZATIONOFMAMMARYSTEMCELLSINTHEMAMMARYANLAGEN，THETRANSPLANTATIONOFMAMMARYANLAGENANDTHEMECHANISMSOFMAMMARYMORPHOGENASISANDDEVELOPMENTINTHEEXPERIMENTSCANPROVIDESOMENEWIDEASANDTECHNIQUEINSTNANENTSONTHECLINICALAPPLICATIONOFSOMEBREASTDISEASESTHERAPYANDTHEMAMMARYREGENERATIONAFTERBREASTABLATEDPARTLYALSOONTHEBREASTMODIFICATIONOFDOMESTICANIMALSFORSOMECOSTLYPROTEINS，ORFORIMPROVEMENTOFTHELACTATIONANDTHEMILKPRODUCTIONTHEPRECISELOCALIZATIONOFPUTATIVESTEMCELLSANDOTHERCELLSWITHINTHEEMBRYONICMAMMATYGLANDWOULDHELPTOELUCIDATETHEMOLECULARSIGNALPATHWAYSTHATGOVERNNORMALMAMMARYDEVELOPMENTTHEULTRASTRUCTURALAPPEARANCESANDTHEANTIGENOFSEA1WERECONSIDEREDTOGETHERASPUTATIVESTEMCELLMARKE∞，ANDTHEANTIGENSOFCYTOKEMTIN，CDL0，MUC一1ANDCD34ASDIFFERENTIATIONMARKERSELECTRONMICROSCOPYWASPERFORMEDTOREVEALTHEULTRASTRUCTUREOFCELLSINDIFFERENTSITEOFTHEMAMMARYANLAGENANIMMUNOFLUORESCANCESYSTEMWASUSEDTEVEALTHEWHOLESTRUCTUREPROFILEOFTHEMAMMARYANLAGENUSINGTHEANTICYTOKERATINANTIBODYTOLOCALIZESPECIFICTYPESOFCELLPOPULATIONSSUCHASSCAI，CD|0，MUC1ANDCD34POSITIVECELLSWITHINTHEANLAGEN，WHICHDISTRIBUTENEARTHEINSIDEEDGE，DISTRIBUTEUNIFORMLYDISTRIBUTEINTHECENTRALREGIONANDDISTRIBUTEINTHESITEOFMESENCHYMESURROUNDTHENECKOFTHEANLAGENRESPECTIVELYWEALSOOBSERVEDUNDERELECTRONMICROSCOPETHATSOMEPALECELLSLIKEPUTATIVESTEMCELLSREPORTEDBYPREVENIENTSCHOLARS，WHICHAMMAINLYDISTRIBUTEDINTHESEA1CELLPOPULATIONNEARTHEINSIDEEDGEOFTHEANLAGEN，HAVEPALESTAINEDNUCLEOPLASMANDC”O(jiān)PLASM，SPARSEORGANELLESCLUSTEREDCLOSETOTHEIRNUCLEUSANDHAVEALACEOFROUGHENDOPLASMICRETICULUMSANDCELLPOLARIZATIONTHERESULTSINDICATETHATTHEPUTATIVESTEMCELLSARELOCALIZEDNEARTHEEDGEOFTHEMAMMARYANLAGEN，THECELLPOPULATIONSWITHDIFFERENTDIFFERENTIATIONDEGREEWERELOCALIZEDINTHECENTRALPARTANDAROUNDTHEEDGEWITHINTHEANLAGENTHEMAMMARYEPITHELIUMOFEARLYLETHALPHENOTYPESCANBERESCUEDBYTHETRANSPLANTATIONOFEMBRYONICMAMMARYGLANDSOTHESEGENESEXPRESSEDINSOMEKEYSTAGESOFMAMMARYGLANDDEVELOPMENTCANBESTUDIEDFORTHECONTINUEGROWTHOFTRANSFERREDMAMMARYANLAGENINTHE‘‘CLEAREDMAMMARYFATDAD”TRANSGENICMICEEXPRESSINGARECOMBINANTHUMANMONOCLONALANTIBODYAGAINSTHANTAVIRUSWEREUSEDINTHISEXPERIMENTTHETRANSGENICMAMMARYANLAGENWERETRANSFERREDINTOTHEWILDFATPADWHOSEMAMMARYGLANDHADBEENCLEAREDANDTHETECHNIQUESOFPER，RTPCR，INSITEHYBRIDIZATION，HESTAINING，WESTUMBLOTTINGANDIMMⅡHISTOCHEMISTRYETCWEREUSEDTODETECTTHEREGENERATEDMAMMARYGLANDTHGRESULTSINDICATEDTHATTHEGENERALREGENERATEDRATEAFTERTRANSPLANTATIONIS444％24／54；THETRANSGENEWASALSOINTHEREGENERATEDMAMMARYANDTHETRANSGENECANEXPRESSINRNAANDPROTEINLEVELS；THEEXPRESSIONQUANTITYANDMANNERWERERELATEDTO

簡介：導(dǎo)通路刺激乳腺上皮細(xì)胞的增殖和分化泌乳期，瘦素可以誘導(dǎo)信號(hào)轉(zhuǎn)導(dǎo)子和轉(zhuǎn)錄激活子STAT5的磷酸化，STAT5是激活B酪蛋白基因表達(dá)的信號(hào)分子，表明泌乳期瘦素對乳蛋白的合成和分泌具有促進(jìn)作用，在泌乳期瘦索還可以誘導(dǎo)STAT3磷酸化，啟動(dòng)乳腺上皮細(xì)胞凋亡，但是瘦素和OBRB在泌乳期的低表達(dá)表明在這一時(shí)期瘦素誘導(dǎo)乳蛋白基因表達(dá)和啟動(dòng)乳腺上皮細(xì)胞凋亡的作用不顯著；退化期，瘦素和OBRB的表達(dá)水平同時(shí)上調(diào)，此時(shí)瘦素專一性誘導(dǎo)STAT3磷酸化，表明在退化期瘦素與OBRB結(jié)合，通過激活JAKSTAT3信號(hào)轉(zhuǎn)導(dǎo)通路啟動(dòng)乳腺上皮細(xì)胞凋亡，這是乳腺組織重建所必需的。關(guān)鍵詞乳腺瘦素；長型瘦素受體；表達(dá)；信號(hào)轉(zhuǎn)導(dǎo)II，

簡介：東北農(nóng)業(yè)大學(xué)博士學(xué)位論文小鼠乳腺中FGF7、FGF10及其受體的表達(dá)與作用姓名崔英俊申請學(xué)位級別博士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師李慶章20071220ABSTRACTEXPRESSIONANDFUNCTIONOFFGF7、FGF10ANDITSRECEPTORINMAMMARYGLANDOFMOUSEABSTRACTINMAMMALIANREPRODUCTIVECYCLE，MAMMARYEPITHELIALSEXPERIENCETHEPROCESSOFREPEATEDGROWTH，DIFFERENTIATIONANDDEGRADATIONTHELACTATIONANDTHEDEVELOPMENTOFMAMM哪GLANDWASREGULATEDBYAVARIETYOFHORMONESANDCYTOKINESTHETHREEMAJORSYSTEMICHORMONESDIRECTLYSTIMULATEDTHEDEVELOPMENTOFMAMM咧GLANDESTROGEN，PROGESTERONE，ANDPROLACTINTHESESYSTEMICHORMONEISPROBABLYTHROUGHTHETISSUEREGULATOR，SUCHASFGFSTOREGULATECELLFUNCTIONALLOFTHEFGFSFAMILYARETHEMITOGENAFFECTINGTHEECTODERM，ENTODERMANDTHEMESENCHYMALCELLSINMAMM哪GLANDDEVELOPMENTPROCESS，KGFRANDITSMATRIXLIGANDFGF7ANDFGF10PLAYANIMPORTANTROLEFORMAMMARYEPITHELIALCELLPROLIFERATIONANDMORPHOGENESISTHEMICEWEREUSEDASEXPERIMENTALANIMALSINTHISSTUDY，THROUGHTHEEXPERIMENTINVITROANDINVIVO，SYSTEMICINVESTEDTHEDYNAMICCHANGESINEXPRESSIONANDLOCALIZATIONOFFGF7，F(xiàn)GF10ANDITSRECEPTORINMAMMALSDEVELOPMENT，LACTATIONANDINVOLUTION；REVEALEDTHERELATIONSHIPSBETWEENTHEEXPRESSIONOFFGF7，F(xiàn)GFL0ANDITSRECEPTORANDTHEFUNCTION；CLARIFIEDTHEFUNCTIONOFFGF7ANDFGF10INMAMMARYGLANDDEVELOPMENT，LACTATIONANDINVOLUTIONANDTHEEFFECTOFMAMMOGENICHORMONESONEXPRESSIONOFFGF7ANDFGF10ANDITSRECEPTORINDIFFERENTPERIODS加VITROTHERESULTSWEREASFOLLOWS1THEEXPRESSIONOFFGF7HADAFEWNOTABLEHIGHPOINTSVIRGIN20D，VIRGIN40D，THEEARLYANDMIDDLEPREGNANCY，EARLYLACTATION，ANDTHEINITIALANDTHELATEINVOLUTIONTHEEXPRESSIONOFFGF7INCREASEDTOTHEHIGHESTVALUEININVOLUTION4DAMONGTHESEPERIODSTHESIGNIFICANTLYHIGHEREXPRESSIONPOINTSOFFGF10WEREVIRGIN20DAND35D，PREGNANCY4DINLACTATION4D，THEEXPRESSIONOFFGF10HADBEENRELATIVELYHIGHANDTHESIGNIFICANTLYHIGHEREXPRESSIONPOINTWASLACTATION10DAFTERENTERINGINVOLUTIONFGFL0WASSIGNIFICANTDECLINEDININVOLUTION4DAND7DANDTHENINCREASEDANDMAINTAINEDAHI曲LEVELEXPRESSIONTHEHIGHEREXPRESSIONOFKGFRINVIRGINWAS20D，60D；INPREGNANCY，THEKGFRLEVELCUTTEDDOWNSLIGHTLYFIRSTANDINCREASEDSIGNIFICANTLYAFTERPREGNANCY10D，THENDECLINEDSLIGHTLYAFTERUPTOPREGNANCY16DINLACTATION，THEEXPRESSIONOFKGFRWASHIGHESTIN4DANDTHENFALLENUNTILLINVOLUTIONTHEEXPRESSIONOFKGFRINCREASEDSIGNIFICANTLYFROMINVOLUTION19DPARTIALCORRELATIONANALYSISSHOWEDTHATTHEEXPRESSIONOFFGF7，F(xiàn)GF10ANDKGFRWERERELEVANTINLACTATION2FGF7MAINLYLOCALIZEDINTHEMATRIXOFMAMMARYGLANDDUCTALEPITHELIALCELLSANDACINARCELLSFGFL0SHOWEDASIMILARLOCATIONPATTERNASFGF7KGFROCCURREDMAINLYINTHEMAMM叫GLANDDUCTALEPITHELIALCELLSANDACINARCELLS，ANDAWEAKEXPRESSIONINMATRIXINTHEINDIVIDUALTIME3FGF7III

簡介：中山大學(xué)碩士學(xué)位論文脂肪源性干細(xì)胞在乳腺整形中的基礎(chǔ)研究姓名楊樺申請學(xué)位級別碩士專業(yè)動(dòng)物學(xué)指導(dǎo)教師陳系古20070520作者楊樺脂肪源性JF細(xì)胞存乳腺整形中的基礎(chǔ)研究1．無菌技術(shù)取SD大鼠雙側(cè)腹股溝脂肪摯，用I型膠原酶消化，獲取脂肪源性干細(xì)胞，接種于含10％胎牛血清的高糖DMEM培養(yǎng)液中，觀測其生長情況，繪制生長曲線，鑒定其表面分子標(biāo)志CD29，CD44，CD45。2．添加脂肪定向誘導(dǎo)劑，將脂肪源性干細(xì)胞誘導(dǎo)成為脂肪細(xì)胞，并用油紅0染色鑒定。3．將經(jīng)過BRDU標(biāo)記并誘導(dǎo)后的脂肪細(xì)胞連續(xù)三次，每次間隔十天，接種于NOD．SCID小鼠一側(cè)皮下，對側(cè)空白對照，觀察小鼠生長情況并在注射細(xì)胞結(jié)束后一個(gè)月和兩個(gè)月取材，觀察組織細(xì)胞形態(tài)學(xué)變化并用油紅0染色鑒定，BRDU免疫組化鑒定。研究結(jié)果1．脂肪源性干細(xì)胞易于分離，培養(yǎng)，具有穩(wěn)定的擴(kuò)增能力，可傳代十次，不易衰減。細(xì)胞表面分子標(biāo)志鑒定結(jié)果CD29，CD44分別為95％，98％，CD45為6％。2．通過添加脂肪定向分化誘導(dǎo)劑，于培養(yǎng)第四天前后細(xì)胞出現(xiàn)脂滴，十天后脂肪細(xì)胞生成率60％以上，細(xì)胞形態(tài)學(xué)和油紅0染色證實(shí)為脂肪細(xì)胞定向分化。繼續(xù)培養(yǎng)可見細(xì)胞變圓從培養(yǎng)瓶底壁游離。3．將BRDU標(biāo)記過并誘導(dǎo)而生成的成熟脂肪細(xì)胞接種入NOD．SCID小鼠一側(cè)背部皮下，對側(cè)空白對照。觀察LID,鼠生長良好，注射細(xì)胞側(cè)可見局部隆起，隆起直徑約LCM，厚約2MM。對側(cè)陰性。經(jīng)組織切片發(fā)現(xiàn)存活于小鼠體內(nèi)的細(xì)胞形態(tài)正常，沒有異型性，經(jīng)油紅0染色證實(shí)為成熟脂肪組織。本實(shí)驗(yàn)分別觀察了細(xì)胞注入NOD．SCID小鼠皮下一個(gè)月和兩個(gè)月后的情況，發(fā)現(xiàn)兩個(gè)月小鼠的移植脂肪細(xì)胞較一個(gè)月小鼠的移植脂肪細(xì)胞直徑增大，表明經(jīng)誘導(dǎo)后的細(xì)胞具有良好的生長能力，能適應(yīng)從體外到體內(nèi)的生存環(huán)境，并隨著環(huán)境的變化而變化，這一點(diǎn)體現(xiàn)在它能隨著小鼠的生長發(fā)育而積聚脂肪，成為成熟脂肪組織。而且因?yàn)楸緦?shí)驗(yàn)中細(xì)胞為多次注入，也為臨床中分離培養(yǎng)細(xì)胞后多次治療提供了一定的參考價(jià)值，同時(shí)也發(fā)現(xiàn)成活的脂肪組織中并沒有明顯的血管的生成，這說明成活的細(xì)胞可能主要是靠局部滲出的組織問液存活，同時(shí)因?yàn)榫植恐镜暮穸葍H為2MM左右，這也間接說明血管化的障礙可能也是影響細(xì)胞生長的重要因素。LL

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文抗菌肽B在奶山羊乳腺上皮細(xì)胞系中的高效表達(dá)姓名尹德云申請學(xué)位級別碩士專業(yè)生物化學(xué)與分子生物學(xué)指導(dǎo)教師高學(xué)軍20080620THEEFFECTIVEEXPRESSIONOFTHECECROPINBINGOATMAMMARYGLANDEPITHELIALCELLLINEABSTRACTTHISSTUDYWASDESIGNEDTOCONSTRUCTTHEEUKARYOTICEXPRESSIONVECTORBYGENERECOMBINATIONTECHNOLOGYANDEXPRESSCECROPINBGENEINTHEGOATMAMMARYEPITHELIALCELLSITAIMEDATTHEEXPRESSIONOFCECROPINBGENEATAHIGHLEVELINTHEMAMMARYGLANDSOFGOATS，ANDPROVIDEDAVIABLETECHNICALMEANSANDCREDIBLETHEORIESONPRODUCTIONOFTRANSGENICANIMALSANDTRANSGENICMILKIMPROVEMENTOFMILKQUALITYFORTHEFUTUREINADDITION，THEEUKARYOTICEXPRESSIONVECTORCONSTRUCTEDINTHISSTUDYCONTAINEDTHEWHOLEEGFPGENESEQUENCE，ANDITSDOWNSTREAMREGULATORYSEQUENCEENDEDWITHRESTRICTIONENDONUCLEASESITES，WHICHPROVIDEDACONVENIENT，RELIABLEMODELFORRESEARCHONDIFFERENTEUKARYOTICGENEREGULATIONSEQUENCESINTHISSTUDYTOTALRNAWASEXTRACTEDFROMIMMUNIZINGSILKWORM，CDNAWASSYNTHESIZEDBYRTPCRREACTIONSANDTHESECDNAWASUSEDASTEMPLATESINPCRAMPLICTIONSTHEPCRPRODUCTSWERE340BP，THESAMELENGTHOFTHEPREDICTEDCECROPINBGENE，ANDWASCLONEDTOPMD18TTOARIALYSISITSSEQUENCE，ANDWASCOMPAREDWITHITSGENEANDAMINOACIDSEQUENCEINGENEBANKTHERESULTSHEWEDTHATTHEGENEANDAMINOACIDSOFCECROPINBHAVEVERYHIGHHOMOLOGYTO100％THEPCRDRODUCTWASLINKEDWITHPMDI8TANDTHERECOMBINANTVECTORWASNAMEDBYPMDI8BTHEPOSITIVERECOMBINANTWASSELECTEDANDDIGESTEDWITHTWORESTRICTIONENZYMES，THEPECFPCIVECTORWHICHHADFLUORESCENCEREPORTGROUPWASDIGESTEDWITHSAMEDOUBLERESTRICTIONENZYMESCECROPINBGENEWHICHHADSTICKYENDSWEREPURIFIEDANDLINKEDWITHPECFPCIWHICHALSOHADTHECORRESBONDINGSTICKYENDSANDWASPURIFIEDTHEPOSITIVERECOMBINANTWASSELECTEDFINALLYBYTHISMEANS，EUKARYOTICEXPRESSIONVECTOROFCECROPINBWASCONSTRUCTEDSUCCESSFULLYITWASNAMEDBYPECFPBTHEN，THEGOATMAMMARYEPITHELIALCELLSWASISOLATEDBYCOLLAGENASEDIGESTIONANDCULTUREDANDSEPARATIONOFCULTUREDGOATMAMMARYEPITHELIALCELLSWASIDENTIFIEDUSINGIMMUNOHISTOCHEMICALSTAININGMETHODTODETERMINATETHEEXPRESSIONOFKERATINI8OFMAMMARYEPITHELIALCELLFURTHERCATIONICLIPOSOMEVECTORSWASUSEDTOTRANSFERTHEPECFPBINTOGOATMAMMARYEPITHELIALCELLS，THUSTHEEXPRESSIONOF3GALACTOSIDASECOULDBEDETECTBYTWOLEVELSMRNAANDPROTEINTHECECROPINBPROTEINWASEXPRESSEDSUCCESSFULLYINGOATMAMMARYEPITHELIALCELLSBYRTPCRANDAGROSRDIFFUSIONTESTTHEVECTORCONTAINEDPECFPCIREPORTGENE，WHICHEXPRESSEDFLUORESCIN，SOTHEGREENFLUORESCENTLIGHTCOULDBESEENINFLUORESCENCEMICROSCOPEFURTHERMORE，THEVECTORCONTAINEDNEE‘RESISTANCEGENESOTHEGOATMAMMARYEPITHELIALCELLSWHICHWERETRANSFECTEDWITHPECFPBWERESCREENEDBYG418OF800MG／MLABOUT3DAYS，AND200MG／MLOVER30DAYSSTEADYEXPRESSIONOFCECROPINBWASCONFIRMEDBYFLUORSCENTTESTTHEEXPRESSIONOFCECROPINBINTHETRANSFECTEDCELLCULTUREMEDIUMANDTHEBROKENCELLSLIQUID

簡介：Y113T596分類號(hào)險(xiǎn)32。嫂壘塑磐旦1JDC一ZI墨密紐壘出學(xué)校代碼；10712研究生學(xué)號(hào)SY20040308西北農(nóng)林釋技夫?qū)W2007屆攻讀碩士學(xué)位研究生學(xué)位畢業(yè)論文R’I’37E幼芽提取物對卵巢、子宮和乳腺上皮細(xì)胞的影日向?qū)W科專業(yè)一絕盥生塑堂指導(dǎo)教師完成刖問墮田五迭塑翌一中國陜西楊凌THEEFFECTSOFEXTRACTSOFMAIZEPLUMPLEONOVARYUTERUSANDMAMMARYEPI唧LIUM【NVITROABSTRACTSUBJECTTORESEARCHTHEEFFECTSOFMAIZEPLUMPLEEXTRACTSONTHEFEMALEREPRODUCTIVEORGANSANDTHEMAMMARYEPITHELIUMINVITROMETHOD①PRODUCEMICEAGEDMODELWITHDG吐MENSTTRATETHEGREENWEIGHTANDAPPARATUSINDEXOFOVARYANDUTERUS，SOD，GSHPX，MDAANDTOTALPROTEINCONTENTINTHETISSUEHOMOGENATEANDESTRADIOLINSERUM②CULTURETHEMAMMARYEPITHELIUMINVITROANDIDENTIFYITBYUSINGCYTOKERATIN18MONOELONALANTIBODYANDCASEINMRNAREVERSETRANSCRIPTION③ADDDIFFERENTCONCCNTRATIONOFMAIZEPLUMULEEXTRACTSTOTHECELLCULTUREMEDIUM，MENSURATETHECELLCURVEOFGROWTH，DOUBLINGTIME，DIVISIONALINDEXANDTOTALPROTEINCONTENT④CULTURETHECELLINSERUNLSTARVEDMEDIUMANDADDDIFFERENTCONCENTRATIONOFMAIZEPLUMULEEXTRACTS，MENSURATETHECELLPROLIFERATIONCELLBREEDINGRATIOANDDEGREEFORPROTECTION；DETERMINETHEBEL一2GENEEXPRESSION⑤CULTURETHECELLINSELUNLSTARVEDMEDIUMANDADDDIFFERENTCONCENTRATIONOFMAIZEPLUMULEEXTRACTS。THENDETERMINETHEEELLTOTALPROTEINCONTENTRESULTS1，THERESULTSOFTHEFEMALEMICEREPRODUCTIVEORGANSTEXTSSHOWTHAT①THECONTENTSOFSOD，GSHPX，TOTALPROTEINANDE2INTHEMICEINJEETEDDGALAREDECLINEDPO01ANDTHEMDACONTENTALEINCREASEDP，OOOEONPAREDWITHTHENOMAL②1HCTWOCONCENTRATIONSOFEMPCANPROMOTETHECONTENTSOFSOD，GSHPXANDTOTALPROTEINSPO01，PO05，REDUCETHEMDACONTENTP001⑧EMPCANINCREASETHEE2CONTENTINSERUMTHEVALUEARE6684O66AND6664O56RESPECTIVELYANDHAVETHESIGNIFICANCECONPAREDWITHTHENORMALP0052THERESULTSOFTHEMAMMARYEPITHELIUMCULTUREDINVITROSHOWTHAT①20MGCLCONCENTRATIONOFEMPCALLSHORTENTHECCUDOUBLINGTIME㈣05ANDINCREASETHECELLDIVISIONALINDEXPO05，ANDHAVETHESIGNIFICANTDIFFERENCE；②MEMPCANNOTINDUCETHECASEINEXPRESSION；③MCELLPROLIFERATIONISREDUCEDUNDERTHESERUMSTARVEDCONDITIONAND20MLAND40MECLEMPHAVETHEPOSTPONABLEEFFECTPO05，ANDHAVETHESIGNIFICANTPROTECTIVEEFFECTP005D11LEMAMLNARYEPITHELIUMCANAPOPTOSISUNDERTHESENUNSTARVEDCONDITIONANDTHEBEL一2GENEEXPRESSES20MG／LAND40MG／LCONCENTRATIONOFEMPCALLINDUCETHEBCL2EXPRESSIONANDTHEAVERAGEOPTICALDENSITYARERO37774O0111ANDO4163400134RESPECTIVELYANDHAVETHESIGNIFICANTDIFFERENTPO05，P001COMPAREDWITHTHESCAUNLSTARVEDONETHE10MG／LCONCENTRATIONARENOTSIGNIFICANT⑤20

簡介：分類號(hào)Q3445學(xué)校代碼10129UDC576學(xué)號(hào)08210053嗜熱菌糖苷酶基因LACS牛乳腺特異性表達(dá)載體的構(gòu)建、轉(zhuǎn)染及鑒定STUDYONCONSTRUCTIONTRANSFECTIONOFMAMMARYGLSPECIFICEXPRESSIONVECTFSSΒGLY申請人杜雪學(xué)科門類農(nóng)學(xué)學(xué)科專業(yè)動(dòng)物發(fā)育生物學(xué)與生物技術(shù)研究方向動(dòng)物發(fā)育生物學(xué)指導(dǎo)教師周歡敏教授論文提交日期二〇一一年六月STUDYONCONSTRUCTIONTRANSFECTIONOFMAMMARYGLSPECIFICEXPRESSIONVECTFSSΒGLYABSTRACTPRODUCINGBIOACTIVEPROTEINSTHROUGHMAMMARYGLBIEACTISAHOTFOCUSINTHEFIELDOFBIOLOGYTHEMOSTIMPTANTQUESTIONISTOCONSTRUCTEXPRESSIONVECTINMAMMARYGLCSNISTHEMAINPROTEININMILKFIRSTLYTHECMVPROMOTERPOLYALACSBCPROMOTERWEREAMPLIFIEDFROMDIFFERENTSOURSESBYPCRTECHNOLOGYFOLLOWEDTHEABOVESEQUENCEARECONNECTEDWITHPDSRED21PLASTOCONSTRUCTMAMMARYGLSPECIFICEXPRESSIONVECTPBLUSINGTHEUPDREAMREGULATIONSEQUENCEOFBOVINEBCREGULATELACSGENEFEXPRESSIONINMAMMARYGLCMVPROMOTERREGULATETHEEXPRESSIONOFREDFLUESCENTPROTEINGENETHEPOLYASEQUENCEISVERYIMPTANTFEXPRESSIONSECONDLYUSETHENEWVECTSTOTRASFACTTHEBOVINEMAMMARYEPITHELIALCELLSIDENTIFYTHECELLSINTODNARNAPROTEINLEVELSTRAININGMONGOLIABOVINEFETALFIBROBLASTCELLSTHECULTUREDCELLSWEREANALYZEDBYTHEIRMPHOLOGYGROWTHCURVERESULTSDEMONSTRATEDTHATTHESECULTUREDCELLSWERETYPICALMONGOLIABOVINEFETALFIBROBLASTCELLSWITHNMALMPHOLOGYTHEPBLPLASVECTCOATEDWITHLIPOFECTAMINEREGEANTWASTRANSFERREDINTOTHEMONGOLIABOVINEFETALFIBROBLASTCELLSTOIDENTIFIEDTHETRANSFERCELLSFROMDNALEVELTHERESULTSSHOWEDTHATTHESENSITIVITYSTUDIESOFBOVINEMAMMARYEPITHELIALCELLSAGAINSTG418CONCENTRATIONINDMEMF12MEDIAINDICATEDASHIGHAS700GMLTHETARGETGENECANBEIDETIFIEDFROMTHREELEVELSMONGOLIABOVINEFETALFIBROBLASTCELLSAGAINSTG418CONCENTRATIONINDMEMF12MEDIAINDICATEDIS700GMLTHETARGETGENEALSOCANBEDETECTEDINTRANSFACTEDCELLSALLINALLTHECONSTRUCTIONOFTHEEXPRESSIONVECTISSUCCESSFULTHECELLSCANBEUSEDTOBESOURCESTOCREATETRANSGENICANIMALSBYNUCLEARTRANSFERTECHNIQUESKEYWDSMAMMARYBIEACT；SSΒGLY；MAMMARYGLEXPRESSIONVECT；DIRECTEDBYPROFZHOUHUANMINAPPLICANTFMASTERDEGREEDUXUEANIMALDEVELOPMENTALBIOLOGYBIOTECHNOLOGYCOLLEGEOFLIFESCIENCES，INNERMONGOLIAAGRICULTURALUNIVERSITY，HUHHOT010018，CHINA

簡介：碩士學(xué)位論文題目敲除敲除抑癌基因抑癌基因PTPROPTPRO對小鼠乳腺發(fā)育的影響對小鼠乳腺發(fā)育的影響英文題目英文題目TARGETEDDISRUPTIONOFTUMSUPPRESSPTPROALTERSMAMMARYDEVELOPMENTINMOUSE姓名殷秀凱學(xué)號(hào)10920023所在學(xué)院汕頭大學(xué)醫(yī)學(xué)院導(dǎo)師姓名張灝專業(yè)生物化學(xué)與分子生物學(xué)入學(xué)日期2009年9月答辯日期2012年5月

簡介：揚(yáng)州大學(xué)碩士學(xué)位論文組織型纖溶酶原激活劑（TPA）突變體乳腺特異性表達(dá)載體的構(gòu)建及其細(xì)胞表達(dá)研究姓名周詠松申請學(xué)位級別碩士專業(yè)臨床獸醫(yī)學(xué)指導(dǎo)教師成勇20080501揚(yáng)州大學(xué)碩士學(xué)位論文揚(yáng)州大學(xué)學(xué)位論文原創(chuàng)性聲明和版權(quán)使用授權(quán)書學(xué)位論文原創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是在導(dǎo)師指導(dǎo)下獨(dú)立進(jìn)行研究工作所取得的研究成果。除文中已經(jīng)標(biāo)明引用的內(nèi)容外，本論文不包含其他個(gè)人或集體已經(jīng)發(fā)表的研究成果。對本文的研究做出貢獻(xiàn)的個(gè)人和集體，均已在文中以明確方式標(biāo)明。本聲明的法律結(jié)果由本人承擔(dān)。學(xué)位論文作者簽名司冰擬簽字日期Y啼年鑰、7日學(xué)位論文版權(quán)使用授權(quán)書本人完全了解學(xué)校有關(guān)保留、使用學(xué)位論文的規(guī)定，即學(xué)校有權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交學(xué)位論文的復(fù)印件和電子文檔，允許論文被查閱和借閱。本人授權(quán)揚(yáng)州大學(xué)可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。同時(shí)授權(quán)中國科學(xué)技術(shù)信息研究所將本學(xué)位論文收錄到中國學(xué)位論文全文數(shù)據(jù)庫，并通過網(wǎng)絡(luò)向社會(huì)公眾提供信息服務(wù)。、／哆學(xué)位論文作者虢間球杠導(dǎo)腳多R窮簽字日期鵬臼IF日簽字日期鵬名月F7日

簡介：西北農(nóng)林科技大學(xué)碩士學(xué)位論文人胰島素基因改造及其山羊乳腺特異性表達(dá)載體構(gòu)建姓名王莉申請學(xué)位級別碩士專業(yè)發(fā)育生物學(xué)指導(dǎo)教師張涌20080501

簡介：論文題目墜丕蘭基囚在堊已避疸生的麥達(dá)型盎座意豎答辯委員會(huì)主席迕坌IJ醫(yī)數(shù)援答辯委員會(huì)成員潘熗墾數(shù)攫郄圭強(qiáng)數(shù)攫隧；巫賢教援但耀空數(shù)援論文答辯日期2Q09二1127溫州醫(yī)學(xué)院佃JIJ學(xué)位論文TBX3基因在乳腺癌中的表達(dá)和臨床意義溫州醫(yī)學(xué)院定理臨床學(xué)院溫州市第■人民醫(yī)院腫瘤外科研究生姓名葉英海導(dǎo)師張?bào)泸懕尘叭橄侔┦浅Ｒ姷膼盒灾?，痛之一。近幾年來，統(tǒng)計(jì)資料表明乳腺癌的發(fā)病率呈逐年上丁F趨勢。乳腺的胚胎發(fā)育和乳腺癌的形成中有幾個(gè)共同之處Ⅲ，都發(fā)生了細(xì)胞增賄，凋亡，侵入周圍組織以及新牛血管的形成。岡此研究乳腺胚胎發(fā)育中的轉(zhuǎn)錄調(diào)控因子，找到其作用機(jī)制，對于闡明乳腺癰的發(fā)牛，發(fā)展町能的致病因素有一定的提示和指導(dǎo)作用。TBX3是TBOX家族的一個(gè)轉(zhuǎn)錄因子，TBOX基因轉(zhuǎn)錄因予以具有高度保守的DNA結(jié)合區(qū)域T區(qū)為特征，都能和靶基因啟動(dòng)子的特定序列相結(jié)合，在脊椎動(dòng)物和非脊椎動(dòng)物的組織形念發(fā)生和器官形成過程中發(fā)揮著至關(guān)重要的作用。所有的TBOX轉(zhuǎn)錄兇子，即T蛋門，均有一個(gè)特征性的結(jié)構(gòu)，即在它們的N端都有一個(gè)由180個(gè)氨皋酸殘翠組成的DNA結(jié)合區(qū)，稱之為TBOX區(qū)，該區(qū)的DNA序列高度保守，而其自仃、后的序列非常多變。TBX3基兇編碼了一條由743個(gè)氨基酸組成的肽鏈，DNA結(jié)合|又化于第105285位氨基酸心1。在人類家族性常染色體顯性遺傳性疾病尺骨一乳腺綜合征U1NATMAMMARYSYNDROME，UMS中對于TBX3基兇及其編碼的轉(zhuǎn)錄因子的研究比較深入。在UMS中一個(gè)TBX3等位基因發(fā)生突變，經(jīng)常造成縮短的非功能性的蛋白合成和野生型TBX3蛋白的不足，也被稱作是單倍劑量不足。功能性TBX3等位基因在尺骨一乳腺綜合征病人中的缺失導(dǎo)致嚴(yán)蕈的先天性疾病或者乳腺上皮組分的缺失L?！耙Ｒ虼嗽O(shè)想若過度的TBX3表達(dá)是否在乳腺癌的發(fā)牛、發(fā)展起到關(guān)鍵的作用。有學(xué)者已經(jīng)證明TBX3可以抑制腫瘤抑制基岡P14ARF的表達(dá)，P14ARF在乳腺癌患者中突變和異常表達(dá)∞7＆9I。TBX3對P14ARF的抑制作用使其阻止雙微體2介導(dǎo)的P53的降解的功能下降，縮減P53的半哀期E10。TBX3基岡可以協(xié)同MYC和RAS癌基因在細(xì)胞轉(zhuǎn)化中發(fā)揮作用，過表達(dá)時(shí)可以抑制細(xì)胞的凋亡。表明TBX3基因在乳腺上皮細(xì)胞中的過表達(dá)可能允許細(xì)胞在抑制生長／增殖的環(huán)境中經(jīng)歷另外一輪細(xì)胞分裂，從而繼續(xù)生長。因此，如果該基因持續(xù)過表達(dá)，上述過程將反復(fù)進(jìn)行，最后導(dǎo)致乳腺上皮細(xì)胞的失控性生長，發(fā)生惡性轉(zhuǎn)化，從而促進(jìn)乳腺癌的發(fā)生。分別有文獻(xiàn)報(bào)道TBX3在乳腺癌細(xì)胞和血清中過表達(dá)生”1。目前針對該基因在乳腺癌組織中的表達(dá)狀況和臨床特征的聯(lián)系研究較少。目的研究TBX3在乳腺癌組織中的表達(dá)情況和在具有不同臨床病理特征的乳腺癌中的表達(dá)差異的臨床意義。

簡介：東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文奶牛乳腺上皮細(xì)胞系的建立姓名陳建暉申請學(xué)位級別碩士專業(yè)基礎(chǔ)獸醫(yī)學(xué)指導(dǎo)教師高學(xué)軍20080620ABSTRACTESTABLISHMENTOFDAIRYCOWMAMMARYGLANDEPITHELIALCELLLINEABSTRACTMAMMARYGLANDEPITHELIALCELLSARESPECIALIZEDSECRETORYFUNCTIONCELLS，ANDAREALSOTHEALLFOUNDATIONOFLACTATIONFUNCTIONMATERIALSFORSTUDYINGONLACTATIONSTARTING，F(xiàn)UNCTIONSMAINTENANCE，DEGRADATIONETC，PHYSIOLOGICALPROCESSINTERNALANDEXTEMALCYTOKINEREGULATION，SIGNALTRANSDUCTION，F(xiàn)UNCTIONGENESEXPRESSIONANDTHEFORMATIONOFMAMMARYGLANDTUMORS，CANCERATIONACCORDINGTONOWADAYSTUDIES，BETWEENRUMINANTSANDHUMAN，LACTATIONCONTROLMECHANISMEXISTINGAGREATDIFFERENCESCOMPAREDTOOTHERSPECIESOFANIMALS，MAMMARYGLANDREGULATIONMECHANISMOFRUMINANTSHASNOTBEENFULLYPROVED，ESPECIALLYTHATTHECULTUREOFLACTATIONDAIRYCOWMAMMARYGLANDEPITHELIALCELLSWHICHHAVEHIGHLEVELSECRETIONOFMILKISDIFFICULTDAIRYCOWMAMMARYGLANDEPITHELIALCELLSCULTURED／NVITROCALLBEUSEDFORRESEARCHOFMAMMARYGLANDDEVELOPMENTLACTATIONMECHANISM，ANDBIOLOGICALREACTORAPPLICATIONSETCALTHOUGHTHEREHAVEBEENSOMEREPORTSOFFCULTUREDDAIRYCOWMAMMARYGLANDEPITHELIALCELLS，RESEARCHERSALWAYSCANNOTSUCCEEDINESTABLISHINGDAIRYCOWMAMMARYGLANDEPITHELIALCELLSLINE，CELLSGAINEDAREUNSUITEDFORTHERESEARCHONLACTATIONMECHANISMANDDETECTIONOFMAMMARYGLANDEXPRESSIONVECTORESTABLISHMENTOFDAIRYCOWMAMMARYGLANDEPITHELIALCELLLINEISFAIRLYGOODASANEXTERNALMODELFORTHEESTABLISHMENTOFTRANSGENICANIMALS，STUDYOFTHELACTATIONQUANTITYIMPROVEMENT，ANDALSOAUXANOLOGYMASTOPATHYNEOCONFUCIANISMANDBIOENGINEERINGETCTHEREFOREITISNECESSARYTOCULTUREDAIRYCOWMAMMARYGLANDEPITHELIALCELL／NVITROANDSTUDYTHEROLEOFHORMONESONMAMMARYGLANDEPITHELIALCELLFUNCTIONTHISSTUDYSELECTEDSLAUGHTEREDHEALTHHOLSTEINDAIRYCOWMAMMARYGLANDTISSUEOFLATESTAGELACTATIONASEXPERIMENTALMATERIAL，ANDUSEDTISSUEBLOCKINOCULATIONMETHODTOCULTUREDAIRYCOWMAMMARYGLANDEPITHELIALCELLSSUCCESSFULLYCYTOKERATIN18WASUSEDFORIMMUNOFLUORESCENCESTAININGOFMAMMARYGLANDEPITHELIALCELLSACCORDINGTOCELLSINOCULATIONSURVIVALRATE，POPULATIONDOUBLINGTIME，GROWTHCURVEMORPHOLOGYANDOTHERBIOLOGICALCHARACTERSOFDETECTION，ADAIRYCOWMAMMARYGLANDEPITHELIALCELLLINEWASESTABLISHEDINADDITIONIACTOSEANDCASEINPROTEINCONTENTSOFDAIRYCOWMAMMARYGLANDEPITHELIALCELLSWEREMEASUREDBYHPLCMETHODRESTIVELYREFLECTINGEFFECTSOFTHEHORMONEONLACTATINGDAIRYCOWMAMMARYGLANDEPITHELIALCELLSFUNCTION，THUSTHISSTUDYGAVEIMPORTANTBASISFORDAIRYCOWMAMMARYGLANDEPITHELIALCELLSBIOREACTORMODELANDSTUDYOFLACTATIONMECHANISMTHEEXPERIMENTALRESULTSSHOWEDTHATDAIRYCOWMAMMARYGLANDEPITHELIALCELLSHADBEENPURIFIED，ANDTHEESTABLISHMENTOFDAIRYCOWMAMMARYGLANDEPITHELIALCELLLINEHADBEENIDENTIFIEDTHROUGHPASSAGEOFTHEMAMMARYGLANDEPITHELIALCELLSANDREPEATEDLYFROZENANDRECOVERYTHEMAMMARYGLANDCELLLINEWASPROVEDTOHAV弓ACHIEVEDIONGTERMPRESERVATION，ANDALARGENUMBEROFMAMMARYGLANDEPITHELIALCELLSWEREGAINEDDAIRYCOWMAMMARYGLANDEPITHELIALCELLSGREWWELLINTHECULTURELIQUIDMEDIUMAFTERADDINGWITHEPIDERMALGROWTHFACTORS，ANDHADGOODCHARACTERSOFPROLIFERATION，ANDTHECELLSOVER20PASSAGESOFPROLIFERATIONREMAINEDEXCELLENTACTIVITYCONTENTSOFCASEINPROTEINOFMAMMARYGLANDEPITHELIALCELLSCULTUREDWITH

簡介：細(xì)胞周期調(diào)控蛋白CYCLIND1基因靶向MICRORNAS的預(yù)測、系統(tǒng)驗(yàn)證及西洋參提取物處理人乳腺癌細(xì)胞的表達(dá)譜分析⑧論文作者簽名指導(dǎo)教師簽名I趁論文評閱人1評閱人2評閱人3評閱人4評閱人5答辯委員會(huì)主席委員1委員2委員3委員4委員5答辯曰期2翌2翌壘益浙江大學(xué)研究生學(xué)位論文獨(dú)創(chuàng)性聲明本人聲明所呈交的學(xué)位論文是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果，也不包含為獲得迸姿盤塋或其他教育機(jī)構(gòu)的學(xué)位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示謝意。學(xué)位論文作者簽名與厶衷未簽字日期2。厶年多月7日學(xué)位論文版權(quán)使用授權(quán)書本學(xué)位論文作者完全了解逝姿盤堂有權(quán)保留并向國家有關(guān)部門或機(jī)構(gòu)送交本論文的復(fù)印件和磁盤，允許論文被查閱和借閱。本人授權(quán)逝姿盤堂可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索和傳播，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文。一保密的學(xué)位論文在解密后適用本授權(quán)書學(xué)位論文作者簽名?；ピ滥?dǎo)師簽名簽字同期砩年苦月7日簽字日期彥。／D年石月7日

簡介：L學(xué)校代碼10571L學(xué)號(hào)2007010012奶牛13一酪蛋白啟動(dòng)子引導(dǎo)的乳腺高效定位表達(dá)載體的構(gòu)建與篩選CONSTRUCTIONANDSCREENINGOFEFFICIENTANDSPECIFICEXPRESSIONVECTORWHICHISGUIDEDBYCOWISCASEINPROMOTER學(xué)科門類學(xué)科、理學(xué)專業(yè)生物化學(xué)與分子生物學(xué)研究方向年級研究生姓名導(dǎo)師姓名起止日期生物制藥二零零七級一令令屯繳邵正江黎明主任技師2007．9～20LO．5廣東醫(yī)學(xué)院生物化學(xué)與分子生物學(xué)教研室二OO年五月資助廣東省自然科學(xué)基金項(xiàng)目,5011595學(xué)位論文獨(dú)創(chuàng)性聲明本人呈交的學(xué)位論文系在導(dǎo)師指導(dǎo)下所取得的研究成果。除了文中特別加以標(biāo)注和致謝的地方外，論文中不包含其他人已經(jīng)發(fā)表或撰寫的研究成果，也不包含為獲得廣東醫(yī)學(xué)院或其他教育機(jī)構(gòu)的學(xué)位或證書所使用過的材料。作者簽名娃日期作者簽名迎￡日期學(xué)位論文知識(shí)產(chǎn)權(quán)聲明本人在就讀研究生期間所完成的學(xué)位論文及其相關(guān)成果，知識(shí)產(chǎn)權(quán)歸屬學(xué)校。本人在畢業(yè)離校前會(huì)將有關(guān)的研究資料和結(jié)果全部上交導(dǎo)師，離校后發(fā)表或使用學(xué)位論文或與該論文直接相關(guān)的學(xué)術(shù)論文或成果時(shí)，署名單位仍然為廣東醫(yī)學(xué)院。學(xué)?？梢詫W(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索，可以采用影印、縮印或掃描等復(fù)制手段保存、匯編學(xué)位論文，可以公布包括刊登論文的全部或部分內(nèi)容。導(dǎo)師享有發(fā)表學(xué)位論文、申請成果和專利的權(quán)利。注保密的學(xué)位論文在解密后適用本聲明。作者簽名笠2豎日期導(dǎo)師簽名遼輦豳日期2業(yè)笸Z