恩諾沙星肺靶向微球的研究.pdf

1、y774604分類號(hào)t密緩；’專目差≮夭等碩士學(xué)位論文單位代碼：10019學(xué)號(hào)：S02296恩諾沙星肺靶向微球的研究StudiesOnlungtargetedmicrospheresofenrofloxacin研究生：奎絲指導(dǎo)教師：肖蕉建熬撞申請(qǐng)學(xué)位門類級(jí)別；塞堂硬專業(yè)名稱；基礎(chǔ)盞醫(yī)堂研究方向：獸醫(yī)葒堡生耋堡堂所在學(xué)院：動(dòng)物醫(yī)堂院2005年6月AbstractEnrofloxacin，allantibacterialdmgmarkete

2、dspecificallyforanimaluse，hasbroad—spectrmnactivity，includingGram—negativeandsomeGrampositivebacteriaErtrofloxacinisusefulforthetreatmentofseveralanimalbacterialdiseaseandmycoplasmapenumoniaEncapsulationofenrofloxacininm

3、icrospheresareusedtoimproveefffectivenssofthedruginthetreatmentofbacterialinfectionsandtoaltersomepharmacikineticpropertiesbyinravenousadministrationAnoptimumprocedurewasestablishedforpreparinglungtargetingenrofloxacinmi

4、scrospheresbythemethodofenmulsification—congealingmethodTheappearance，particleandsizedistribution，ENRcontent，invitrelease，stability，determinationofenrofloxacininrabbitandlungtargetingcharacteristicswerestudiedinthepaperE

5、nrofloxacinwaschosenasmodeldrug，biodegradablegelatinwasused∞acarriermateral，liquidparaffin∞theoilphaseSpan一80a3theemusifier111eonhogonaltestmethodswasalsousedtooptimizethepreparationconditionoftheblankgelatinmicrospherer

6、sFurthermore，Lungtargetedmicro；pheresofenrofioxacinwerepreparationbyusingtheemulsifyingtechniqueTheresultsindicatedthatarithmeticmeandiameterofENR—GMSwas1235umwith92％oftheENRGMSrangefrom70to30umThedrugcontentinthemicrosp

7、hereswasdeterminedbyultravioletspectrophotometryTheaverageENR—GMSentrapmentefficiencywas4362％andthequantityofloaddrugwas2067％ReleaseofthedrugfromENRGMSinvitremarkablybecameslowerthanthmofENRanditsty2was6timeslongerthanth

8、atofENR，ThedissolutionprofilesoftheENRGMSfollowedHiguchikineticsThereleaserateofenrofloxacininvitroshowedthattheENRGMShadpronouncedlysustained—releaseeffectThestudyonthestabilityoftheENR—GMSincludedthelightacceleratedtes

9、tandthermalstresstestAllcharacteristicsuchaScolor，particleandsizedistribution，ENRcontentanddecomposedproducthadnosignificantchangesTheexperimentalresultsindiacatedthatthepreparationhadgoodstabilityPlasmaandtissueENRwered

10、eterminedbyHPLC—FLDThestandardcurveforENRwaslinearinarangeof10～1000ng／mIr09993，n=5，Recoveriesweremorethan70％forENRwith30～300ng／mlfortifiedsamplesThecoeffientofvariationwerelessthan6％Intheresearchofpharmacokinetics，asingl

11、edoseofENRinjectionandthatoftheENRGMSinjectionweregivento22rabbitsbyintravenousadministration，pharmacokineticanalysiswascarriedoutbyusingacomputerprogram(3P97，ChinaPharmaeolohyAssoeiation)Thedistributiontestinrabbitsindi

12、catedthatthelungtargetingeffectoftheENR—GMSwasobviousAfterENRwaspreparedtomicrospheresTargetingefficacy(te)ofthelungcomparedtobloodandothertissuesallweremorethan10，ENRGMSsignificallyalterthedistributionofenrofloxacininra

13、bbitsComparedtootherorgansandbloodthetargetingefficiencyofthelungincreased16to492timesTheKineticsoftheENRingectionandENR—GMSinjectioninrabbitlungafterintravenousinjectionwasbestaccordinglydescribedbytwocompartmentopenmod

14、elandthree—compartmentopenmodelTv2。andTmooftheENRGMSwereextended，itsTⅢwasdelayed，CL(s)wasreducedby522％ENRGMShadagoodtargetingefficiencyKeywords：enrofloxaein，gelatinmicrospheres，rabbit，pharmacokineties，Lungtargetingdelive