NRAGE基因甲基化調(diào)控其在乳腺癌細(xì)胞中的表達(dá).pdf

1、南京師范大學(xué)碩士學(xué)位論文NRAGE基因甲基化調(diào)控其在乳腺癌細(xì)胞中的表達(dá)姓名：黃銀華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物學(xué)；細(xì)胞生物學(xué)指導(dǎo)教師：溫傳俊20110525AbStraCtBacI【ground&objecti、，e：Ⅻ姐GE(neur0拄ophinrec印由0rp75一面船aa曲gMAGEhomolo曲isamen如erof也emelano嗽枷gengene(MAGE)陸niUmeXpressionofmostMAGEproteins

2、isre蚵ctedt0c趾cerandteStis；llo、ⅣeverNRAEGis∞【pressedillmanyno加1altis吼嗆si11eadydeVelopInentand敏hlltStageThestudydemons眥ed廿：斌NRAGEgeneiIlducedceUapoptosisandiIlhibitedcaIlCermetaS切sisNRAGEn珂act嬲apotendalt吼orsuppressorgeneCp

3、Gislandtb幣emetllyla丘onisemer西ng嬲animporta：ntmechaIlismforinac_tiVa=吐onoftumorsuppressorgenesinthecarCiIlogenesisOur陽(yáng)ldyfocusesontherelatioIlsllipb咖eenthereg山ationofNRAGEexpressionandmeulyl撕0nstat嚙i11itspromo白既Methods：啊1e

4、RealtimePCRme血0dwasusedt0d或eCtthemRNAlevelsofⅫ認(rèn)GEgenein也ehi出ymetas謝cbreastc姐CerceUlineMDA—MB231andrelativelylowm能lSt撕cbreaStcancerceUlir屺MCF7TheexistcnceofCpGislandofNRAGEgenewasanalyzedbymeM砒ylPrimerExpress@So血vare(AppI

5、iedBiosySteIns)Hum趾breastc趴cercelll斌s№A—MB一231a11dMCF7w訛treatedw池也edem甜lylath瑪agent5一Aza_CdRneeXpression0fM認(rèn)GEw嬲mendete∞cdby仕les鋤i一刪tatiVepolym衄lsechainreaction(RTPCR)andWestemb10t觚alysisThem抽ylation0fⅫ認(rèn)GEgenein齔twokmsof

6、celllhsw∞dete涮by也emc也ylationSpecificpol徊e粥ech血reaction(MSP)R電sults：TheeXpresSion0fNRAGE刪剛AleVelinMDA—MB一231cellsw觴about40％higher曲nt11atiIlMCF一7cellsCpGislallde)【istedillthep∞moterre百onofⅫ良AGEgenenleRT—PCRandWes屯ernb10tan

7、以ysisshowcd也atNRAGEeXpression、Ⅳ觴illcreaSedsi鰣ficannyinMDAMB一23lcensbutremailledthesa】眥inMCF一7cellsaRerexposuret0thedem利時(shí)1a血培agent5AzaCdRTheCpGislandofNRAC范waShypemetllylatedinMDAMB231cells趾dhypome廿1yla：tedi11MCF7cellsbyM

8、SPConclusion：Me也yl撕onofNRAGEgenecanregulateitseXpressioninmebreastcallcercelllines0哪studyalsoshows吐latmem劬yl撕on渤tuSofNRAGEgeneisrelatedt0m鮑斌aSisofbreaStcancercell1ines1KssuggeSts也atNRAGEcanbeIlsed鈾aclinjcalmoleclllarbiom