核移植介導(dǎo)生產(chǎn)轉(zhuǎn)基因延邊奶山羊供核細(xì)胞體系的研究.pdf

1、In current study，one of the main challenges that all the biologists face is to overcome the problem of tissue heterogeneity for understanding the organ functions further. It is crucial to identify which cell populations

2、produce specific molecules or to get relevant expression profiles reflecting in vivo.In order to study the characteristics of growth mammary gland cell in Yanbian dairy goat，we had established an dairy goat mammary epith

3、elial cell (DGMEC) line of in vitro. Mammary tissues of Yanbian dairy goats were got by conventional operation method and dispersed and cultured in a medium containing fetal bovine serum.Epithelial cells were enriched by

4、 digesting with 0.25％ trypsin repeatedly to remove fibroblast cells and were identified as epithelial origin.The DGMECs displayed monolayer，cobble-stone，epithelial-like morphology，and formed alveolilike structures and is