乏氧通過腫瘤干細胞途徑引起腦膠質(zhì)瘤細胞放射敏感性改變的初步研究.pdf

1、蘇州大學碩士學位論文乏氧通過腫瘤干細胞途徑引起腦膠質(zhì)瘤細胞放射敏感性改變的初步研究姓名：馮麗波申請學位級別：碩士專業(yè)：腫瘤學指導教師：田野陸雪官20090401乏氧通過腫瘤干細胞途徑引起腦膠質(zhì)瘤細胞放射敏感性改變的初步研究英文摘要ThePreliminaryStudyonRadiosensitivityofGliomaCellsinHypoxiathroughCancerStemCellAbstractobjective：Tllisst

2、udywasdesignedtoobservewhethermicroenvironmenthypoxiacouldinfluenceradiosensitivityofgliomacellsthroughcancerstemcellMethods：SHG44andU251gliomacellswereculturedinserumbasedorserumfreecellgrowthmediumat20％or1％02for12and24

3、hrsinvitrorespectivelyThecellsexpressingCD133weremeasuredbyflowcytometryTheinvitrocolony—formingassaywasusedtoinvestigatetheradiosensitivityResults：(1)SHG44andU251gliornacellsculturedinserumfreecellgrowthmediumcontaineda

4、higherfractionoftumorcellsexpressingCD133andhadlowerradiosensitivitythanthecellsculturedinserumbasedmedium(2)ThesetwotypesofgliomacellsculturedinserumbasedmediumcontainedahigherfractionoftumorcellsexpressingCD133andhadlo

5、werradiosensitivityunder1％02for12or24hrscomparedto20％(3)ThesetwotypesofgliomacellsculturedinserumfreecellgrowthmediumcontainedahigherfractionoftumorcellsexpressingCD133andhadlowerradiosensitivityunder1％02for12or24hrscomp

6、aredto20％Conclusions：(1)ThefractionalenrichmentofstemcellsdecreasedradiosensitivityofSHG44andU251gliomacells(2)Whetherinserumfreecellgrowthorserumbasedmedium，thefractionalenrichmentofstemcellsbyhypoxiacouldincreaseradior