CpG-ODN對豬免疫增強(qiáng)作用的研究.pdf

1、萊陽農(nóng)學(xué)院碩士學(xué)位論文CpGODN對豬免疫增強(qiáng)作用的研究姓名：徐文申請學(xué)位級別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：王金寶20060601菜鞠農(nóng)學(xué)院碩士學(xué)諺論文ImmuneE佰ectsofCpG—oDNinPigsABSTRACTBac捃南【lDKAhasmmun。s蛀mul器幻搿0f凳ctsa萎d列aptiveimmu黼持sp。玎sesi玨nme瓣璐speeiesfthadbeenpr0VedthatsyntheticCpGODNmOtIf

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3、。hh鑫d氌e孫s毫i嫩m疆n。髑hance哦e璀e狳cta皤elon醴難op瓣轉(zhuǎn)18羊vee鼬，PigletswereVaccinatedwiththerecombinantplaSmidsand址cenuatedvaccineofCSFVondifFerentdosagestoconnrmtherelationsbetweenthedosagesandimmuneonhancementef詫c塢1ThreekindsofCpGODN

4、motifswhichcontain57一purpu卜CG—pyrpyr3’motifswemsynthesizedanddeeora士ed啊1eywe糟ATeGA，琿汀℃G炎tGACG蕊AeG下鼉AAeGrAAeGTTt2Pi垂啦we砧vacc；na把dw|tha娃enualedvaccineofcsFVa耐tltfeekl耐sofepGoDNmotlfSThenthetiterofspecincantibodjestoa他nuate

5、dVaccineofcsFV，懶eproli詫mtionoflyTnphoc她s，thepercentageofANArlymphocytesandtheconcenIrati徹ofIL2wercassessedTheresultsshowedthattheabove稍entionedimmuneresponses“pi舀如拇a程enua￡edvaccineofCsFVwitb搬reckindsofe西一oDN蠢d囂dw懈s釃|毛。翹鼉

6、lys鼬鞋辮臻躺only戤n強(qiáng)翻蘸僦cineofeswa嬲重|leimmu辯responseofpigletstovaccinewrththec∞motif(ATcGATATcGAT)甜dedwaSthestrongestamongthethreekindsofCpGmotifS3onekind。fCpGmotjfanditscomplemenla哆chainwerepai他dinIoCpGoDNmOnomerandth肌t沁m。no斑

7、erw鑫sclon耐nlop醚D8量V黯靜￡強(qiáng)e陀eo確i羥鞴￡w囂de挺c矧努dol琵犯s撼e|lo玨翱巧memethodands。q吐enedTheresultsindlcafed懶atCpGoDNwassuccessfullyclonedimDpMD饞Tvecto‘4PigletswereinoculatedwithpIasmidsandanenuatedvaccineofCSFVsimultaneouslyandthedosag

8、esofrecombinantplasmidswere100雌，300鳩，600鵂，900雌，1200峙嗽|pectivelyTheresu慪showed業(yè)a￡也eimmune糙s妒nsesofthepigle主sV8ce黼【tedwim6∞粥plasm討sw瑟stronger搬黼氆eo如erdosages1(eywords：CpG—ODN；immuneadjuVa?。籭mmunercsponseianonuatedvaccineofC