34326.中國(guó)鹵蟲(chóng)不同發(fā)育時(shí)期的差異蛋白組學(xué)研究

1、遼寧師范大學(xué)碩士學(xué)位論文中國(guó)鹵蟲(chóng)不同發(fā)育時(shí)期的差異蛋白組學(xué)研究姓名：李妍申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：海洋生物學(xué)指導(dǎo)教師：侯林20080501AbstractContent：ArtemiasinicabelongstoPhylumArthropoda，ClassCmstacea，SubclassBranchiopoda，OrderAnostaca，F(xiàn)amilyArtemiidaeandGenusArtemiaItlivesinthehigh—

2、saltsaltlakesandsalternswhichexistinShanxi，Tibet，XinjiangQinghaiandInnerMongoliaInthelastfewyears，alongwiththecompletionofthegeomeproject，asamilestone，hasprovidedabroaderplatformforlifescienceHowever，biodiversityandcompl

3、exitysuggeststhatstayingoninformationofgenomeisstillfarawayfromexplanntionofmechanismoflifeAsthefinalproductofmostgenes，proteinsaxethefunctionalunitsinthehierarchicalregulationcascadeofgenesIntensivestudyonproteinsisther

4、equirementoffurtherunderstandingofthemechanismsoflivesTheproteomicsresearchofArtemiasinicahasgraduallybecomeoneimportantpostgenomeresearchfocusInestablishingtheproteomicresearchplatformofArtemiasinica，weinvestigateprotei

5、ncomponentsoftheArtemiasinica，aswillhelppeopleunderstandArtemiasinicaonproteinlevelUsingtwo—dimensionalgelelectrophoresis(2一DE)andimageanalysissystem，thechangesof2DEpatternofproteinfromdifferentstagesofembryoofArtemiasin

6、icawereinvestigatedandanalyzedAtotalof97specificproteinspotswereacquiredbyimageanalysissoftwareTenspecificexpressedproteinsWererecognizedasdescribedpreviouslyexceptthatpeptidefragmentswereindentifiedbymatrixassistedlaser

7、desorption／ionizationtimeofflightmassspectrometryPeptidemassmappingoftheselectedspotsledtotheidentificationoftenproteinsspecificexpressedinArtemiasinicaproteins，amongofwhichninespotswereup—regulatedandonespotswere：downre

8、gulatedNineproteinswereidentifiedusingMALDI—TOF—MS觸loftheindentifiedproteinswereclassifiedmainlyintofourcategories：(a)heatshockprotein；(b)factors；(c)enzymes；(d)otherproteinsTherewereexisteddifferentiallyproteinexpression