黑曲霉木聚糖酶的發(fā)酵、性質及其應用研究.pdf

1、東北師范大學碩士學位論文黑曲霉木聚糖酶的發(fā)酵、性質及其應用研究姓名：李丹申請學位級別：碩士專業(yè)：生物化學與分子生物學指導教師：劉東波20040501AbstractTheinfluenceofconditions(pH，temperature，carbonsourcesandothers)OilthesynthesisofxylanagesproductionofAspergillusNigersb02withsolidstatefer

2、mentationandliquidfermentationwerestudiedWhenthegrowthofsb一02inshakeflasksat28℃for96hourswithinitialpH60and2％branonhemicellulosesubstratesitshowedhi曲activityofxylanase。鼴leoptimalpHandtemperatureforthecrudeenzymefiltratea

3、ctivityofsb一02arepH484555℃Itisclearfromthesedatathatsolidfermentationwasabletoincreasethexylanaseactivitycomparedwithliquidfermentation。Sowecartmakeaconclusionthatsolidstatefermentatiollispriorto1iquidfermentation弧棼xylan

4、asebelongstothehydrolytic強zynlesthatcalldecomposethexylantoformxylandietaryfibreandoligo—saccharideTwoXylanasesofAspergillusNigersb02XI、X11werepurifiedbycentrifugationuRrafiltrationgelSephadexG一75andpreparationG。lelectmp

5、horesis?；rangeofoptimaltemperatureandoptimalpHofXI、XIIforhydrolysisofxylanwere4555℃andpH485。5respectively。DatafromtheSDSGelelectrophoresisindicatedthemolecularweightofXI、XIIarc22，000and40，000respectively強eCDspectrtmasho

6、wedthat出eB——oleatodsheetstructureofXylanasesXI、X11werethemainconformationwithXylanasesactivitiesAfterthecorncortexpowderwastreated蛾鏹|％NaOHsolution，itisdecomposedbyxylanaseandeellulaseunderdifferentconditionsTheenzymicpro

7、ductwereanalyzedbyusingscpharoseGel6B。theresultsofthispolymerizationshowedthatthedepolymcrizationofxylanfollowedarandommechanismandthedegreeofthedepolymerizationincreasedasenzymicreactiontimeisprolonged。Itcouldbeconclude