小黑楊莖和葉的基因差異表達(dá)分析.pdf

1、東北林業(yè)大學(xué)碩士學(xué)位論文小黑楊莖和葉的基因差異表達(dá)分析姓名：吳麗麗申請學(xué)位級別：碩士專業(yè)：林木遺傳育種指導(dǎo)教師：姜廷波201106東北林業(yè)大學(xué)碩士學(xué)位論文AbstractPopulussimoniixPnigraisthehybridspeci岱fromthecrossingofPopulussimoniiandPopulusnigra，becauseitsquicklygrowingandgoodwoodquanlityitWaswi

2、delyusedforboardmaterialsandpaperindusFortheunderstandingofwoodformationmechanismofpoplarthedifferentexpressedgenesbetweenstemsandleavesofPopulusimoniixpnigraweledetectedbythecDNAAFLPmethodResultsshowedthatatotalof4192ge

3、nefragmentswereamplifiedandobservedbyLicor4300DNAanalyzerfromthecDNAofsternsandleaves，atotalof2275genefragmentsweredifferentialdisplayedgenesbetweenstemsandleavesInwhich1039genefragmentsWerefromsternand1236genefragmentsw

4、erefromleavesAtotalof402differentiallyexpressedgenefragmentswe他foundbysilverstainingmethodand217uaiquesequences晡哪obtainedanddepositedinGenBankasaccessionnumberJG567410JG567590andGW863844GW863890Blastxanalysisshowedthat16

5、5genefragmentsencodedknowfunctionproteins，and25genefragmentsencodedunknownfunctionproteinsand27genefiagmentsdidnotfoundhomologoussequenceinGenBankBased011theirputativeproteinfunctions，the166genefragmentswerefurtherclassi

6、fiedinto12functionalcategorieswhichincludingmetabolismligninbiosyntheticprocess，transportmicrotubulepolymerizationcellwallbiogenesis／degradationcellulosebiosynthesis，catabolicprocess，transcriptionr銘ulationsignaltransduct

7、ionproteinbiosynthesis，photosynthesisandotherBasedontheanalysisofbioinfonnationfoundthatgenefragmentofS一03waslaccasegeneassociatedtigninsynthesis，whichsharehigherhomologouswithothergenethe1413bpeDNAfragmentoflacxasegenei

8、nPopulussimoniixpnigranamedPsnLACwasobtainedusingRTPCRwhichincludinga1020bpopenreadingframeandencoding339mniIloacidresiduessharinghigherhomologywithotherplantlaccascgenesThePsnLACgeneWasintroducedintotobaecobyAtumefacien