CEM15基因的生物信息學(xué)分析.pdf

1、分類(lèi)號(hào)密級(jí)UDC學(xué)位論文CEM15基因的生物信息學(xué)分析基因的生物信息學(xué)分析（題名和副題名）王翊（作者姓名）指導(dǎo)教師姓名馬義才馬義才副教授副教授電子科技大學(xué)電子科技大學(xué)成都成都（職務(wù)、職稱(chēng)、學(xué)位、單位名稱(chēng)及地址）申請(qǐng)學(xué)位級(jí)別碩士碩士專(zhuān)業(yè)名稱(chēng)生物醫(yī)學(xué)工程生物醫(yī)學(xué)工程論文提交日期2005.05論文答辯日期2005.06學(xué)位授予單位和日期電子科技大學(xué)電子科技大學(xué)答辯委員會(huì)主席堯德中堯德中教授評(píng)閱人2003年1月13日注1注明《國(guó)際十進(jìn)分類(lèi)法UD

2、C》的類(lèi)號(hào)CEM15基因的生物信息學(xué)分析IIAbstractRecentlytheresearchonVifhasrevealedanovelcomponentofinnateimmunesystemAPOBEC3G（CEM15）whichisacytidinedeaminaselethallyhypermutatesretrovirusesretroelements.APOBEC3GthatbelongstoAPOBECfamilya

3、ctsinDNAeditingtoblockthereplicationofretrovirusessuchasHIV1.InfactAPOBEC3GisabletodefenseawidespectrumofdistantlyrelatedretrovirusesinterfereswithHBVthroughadifferentmechanismfromHIV.APOBEC3G（CEM15）isacellularproteinreq

4、uiredfresistancetoinfectionbyhumanimmunodeficiencyvirustype1hasbeenshowntobeacytidinedeaminasewhichisincpatedintovirionssubsequentlytriggersmassivedeaminationofdeoxycytidinetodeoxyuridinewithintheretroviralminusstrcDNA.V

5、ifwhichisaminproteinencodedbyHIV1oftenplaysanimptantroleinviralreplicationofHIV1.OnlyinthevirusproducingcellVifexpressionisnecessaryinitsabsenceinfectionofasubsequenttargetcellterminatesatapostentrystepthroughtheactionof

6、aninnateantiviralmechanism.Vifproteinhastwodomains:onebindstoAPOBEC3GtheotherregulatesthedegradationofAPOBEC3GbyaconservedsequenceSLQ(YF)LAmotif.VifproteininducesrapiddegradationofAPOBEC3Gbyubiquitinationwhichisaproteoso

7、medependentpathway.AsCEM15maybeanewwaytocureAIDSsoitisveryimptanttopeople.Butwenowknowlittleaboutitweshouldanalyzeitcarefully.Inthispaperwedothisbybioinfmaticsways.ThegenewasanalyzedfPIMwAminoacidcontentHyhobicicyprotein

8、secondarystructuremotifdomainsuperfamilyelectronicexpressionpatternPositiononChromosomebybioinfmaticsmethods.Theiginalityofthedissertationisasfollows:1)ThedissertationreviewstheresearchofCEM15(APOBEC3G)whichwaythisgenema