胸腺素β4在Tenon囊成纖維細胞中的表達及CTGF對其表達的影響.pdf

1、華中科技大學碩士學位論文胸腺素β4在Tenon囊成纖維細胞中的表達及CTGF對其表達的影響姓名：穆歌申請學位級別：碩士專業(yè)：眼科學指導教師：曹陽201105華中科技大學碩士學位論文華中科技大學碩士學位論文2ExpressionofThymosinbeta4inHumanTenon’sCapsuleFibroblaststheEffectofCTGFonitsExpressionCidate:MuGeSuperviss:Prof.CaoY

2、angDepartmentofOphthalmologyUnionHospitalTongjiMedicalCollegeHuazhongUniversityofScienceTechnologyAbstractPurpose:Thymosinbeta4(Tβ4)isamainactinsequestratingpeptidethatmodulatesinflammationhealingindifferenttissues.Thepu

3、rposeofthisstudywastoinvestigatethemolecularbiochemicalexpressionofTβ4inhumanTenon’scapsulefibroblasts(HTFs)toinvestigatetheeffectofconnectivetissuegrowthfact(CTGF)ontheexpressionofTβ4.Methods:TotalRNAfromcultured34passa

4、geHTFsinvitrowasextractedwithTRIZOLreagent.Tβ4messengerRNAwasmeasuredbyreversetranasepolymerasechainreaction(RTPCR).AnimmunohistochemicalstainwasusedtodetectTβ4protein.Aftertreatedwith0ngml（control）1.010.0100.0ngmlCTGFf2

5、4h，Tβ4expressionincultured34passagehumanTenon’scapsulefibroblastswasmeasuredbysemiquantitativeRTPCR.Results:AsingleRTPCRamplifiedproductwasobtained.Tβ4immunohistochemicalstainingwaspositiveincytoplasmofHTFswhilenegativei

6、nnucleus.TheresultofthesemiquantitativeRTPCRwasanalyzedbyimageanalysis.Respectivelytreatedwith1.010.0100.0ngmlCTGFthevaluesofTβ4βactinwere1.110.04，1.050.03，0.820.06.Thedifferencebetweenthesetreatedgroupsthecontrolgroup(1