自身免疫性大皰性皮膚病自身抗體靶抗原的分析.pdf

1、安徽醫(yī)科大學碩士學位論文自身免疫性大皰性皮膚病自身抗體靶抗原的分析姓名：王培光申請學位級別：碩士專業(yè)：皮膚性病學指導教師：張學軍199812012AbstractTheanalysisoftargetantigensdirectedagainsteutoantibodiesinautoi皿uneblisteringskindiseasesAutoimuneblisteringskindiseasesincludepemphigus、pe

2、mphigoid、epidermolysisbullousaacqusita、bulloussystemiclupuserythematosus，andeta1ThetargetantigensdirectedagainstautoantibodiesinthesediseaseshavenotbeenelucidatedOurstudiesaredividedintofourmainsinthefollowing：1prelimina

3、ryexplorationofmethodsextractingpemphigusantigensfrombovinetonguemueosa2analysisoftargetantigensdirectedagainstautoantibodiesinpemphigus3analysisoftargetantigensdirectedagainstautoantihodiesinbullouspemphigoid4analysisof

4、targetantigensdirectedagainstautoantibodiesinlinearIgAbullousdermatosis1Thepreliminaryexplorationofmethodsextractingper珥ohigusantigensfrombovinetonguemUCOSaTheepitheliaofbovinetonguemucosaseparatedin1mol／Lsodiumchloridew

5、ereusedasthesourceofpemphigusantigens10casesofserafrompatientswithpemphigusvulgariswererespectivelydetectedwiththeextractspreparedwithmethodsIandIIbyimmunoblottingTheresultsshowedthatIgGclassantibodiesin8casesofserareact

6、edwith165KDand150KDpolypeptides，inwhichIgGclassantibodiesin3of8casesalseactedwith85KD、80KDand70固polypeptidesintheextractpreparedbymethodsIHowever，IgGclassantibodiesin7casesofserareactedwith260KD、230KD、210l①、190KD、180①and

7、160KDpolypeptides，inwhichIgGclassantibodiesin6of7casesreactedwith210皿polypeptidesintheextractpreparedbymethodsIIItwassuggestedthatthemethodswerepractical，andtwomethodshaddifferentinfluenceontheactivityofpemphigusantigens

8、2TheanalysisoftargetantigensdirectedagainstautoantibodiesinpemphigusTheiII!munoblottingoftheextractpreparedbymethodsI1wasperformedwith15casesofpemphigusseraTheresultsshowedthatIgGantibodiesinIicasesofPVserarecognized210K