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1、Glutamine is a non essential amino acid but it is essential for cell proliferation andits presence is required for the smooth process of cell signaling pathways.Themechanisms of differences in protein expression changes
2、and functional annotationduring re-supplemented glutamine it is still not fully understood.To identify themolecular pathways associated with glutamine signaling pathway, we performed bioinformatics analysis of glutamine
3、deprivation and re-supplemented glutamine in HepG2 cells using iTRAQ approach (isobaric tags for relative and absolute quantitation).The samples of cells were harvested for extract and cleaved with trypsin and the result
4、ingpeptides were labeled with iTRAQ reagents.Labeled peptides separated by strong cationexchange and reversed phase LC and analyzed by MALDI-TOF/TOF MS.Proteins identification were performed by using Mascot search engine
5、 (Matrix Science, London,UK; version 2.3.02) against database containing the related sequences.
The results of this study found 10 unique proteins were identified differentiallyexpressed in HepG2 cells.Proteins foun
6、d to be associated with their functions on GFPO,TRSB, EPC, LTM, AATM, and SMBTC, even found significantly up to down regulatedproteins ALDH8A1 and ADH1C during both treatments.We conclude that the upregulated proteins in
7、duced by glutamine deprivation because over-expression of theproteins due to metabolic stress.Instead, re-supplemented glutamine, the cells nutritionalneeds can be distributed and fulfilled, so that the expression of pro
8、teins associated withthe pathways going down again.In summary, our finding provided a comprehensiveprotein profiles relevant to the signaling pathways of glutamine deprivation and resupplemented glutamine in HepG2 cells.
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