釀酒葡萄皮渣中反式白藜蘆醇的提取純化及其抗氧化性的研究.pdf

1、東北農(nóng)業(yè)大學(xué)碩士學(xué)位論文釀酒葡萄皮渣中反式白藜蘆醇的提取純化及其抗氧化性的研究姓名：畢海丹申請學(xué)位級別：碩士專業(yè)：食品科學(xué)指導(dǎo)教師：張永忠20060620StudiesonExtraction，PurificationAndAntioxidantActivityofTransresveratrolinBrewingGrapeWineDregsABSTRACTInthisexperiment，itisaimedchieflyatdevel

2、opingandutilizingskinandresiduesofgrapewhichWaSwinebyproductinindustrialproduction，exploringthetechnologyofreclaiminggans—resveratrolfromit，andresearchingthephysiologicalactivityTheextractionandpurificationmethodsoftrans

3、resveratrolwereresearched，andweresearchedantioxidantactivityoftransresveratr01Throughresearching，itgivetheoreticalbasisandsomescientificmessagefortherecyclingofskinandresiduesofgrapeThedeterminationmethodsoflaansresverat

4、r01WaSestablishedTrans—resveratr01wasdeterminedbyhi曲performanceliquidchromatography(HPLC)Theoptimalchromatographyconditionswereobtainedwithmethanol／water(45：55，v／v)asflowphaSe，flowratio(08mL／min)；columntemperaturechoosed

5、roomtemperatureUndertheseconditions，theseparationeffectWaSpreferable，agoodpeak—shapeWaSobtained，thelinearrelationshipWaSexcellentwithconcertrationoftransresveratrolintherangeof199／mL～2099／mL，theminimumdetectionlimitwas19

6、9／mLTrans—resveratrolWasdeterminedbyspectrophotometrymethod，thebiggestabsorbtionwavelength(306nm)werefirstlyobtained，theabsorbancepresentedalinearrelationshipwithconcentrationoftransresveratrolintherangeofO～8“∥mLThesolub

7、ilityexperimentsWaSestablishedindifferenttemperatureandsolventTheresultsshowedthattrans—resveratrolhadthebestsolubilitywhenacetoneWaSsolventduring20“C～60“CThestabilityexperimentsoftransresveratrolshowedthattheUVspectrumw

8、asgreatlychangedaftertrans—resveratrolWasplacedinUVlightorsunlightfor15minute，transresveratrolwasbadlydestroyedTransresveratrolshoweddecreasingtrendduring24hoursevenifwithoutlighting，buttotallosscontentWasmuchsmallerthan

9、inthesunlightSoweshouldadoptavoidingsunlightmethodwhenextractionandpurificationoftransresveratrolwasmade，anditisverynecessarytousedryingintheshMeforrawmaterialsotherwiseitwoulddes仃oymuchtransresveratr01Transresveratrolwa

10、sextractedbyalcoholrefluxextractionandultrasonicextractionfrombrewinggrapewinedregsTheappropriateconditionsofalcoholrefluxextractionwereobtained，namelyextractedfor5hbyusing80％ethanolsolutionat60。Cwiththematerialratiolof1

11、：20(g／mL)Thecontentsoftrans—resveratrolWaS94299／gTheoptimalprocessofultrasonicextractionwereobtained，namelyextractedfor50minbyusing80％ethanolsolutionat350Wwiththematerialratiolof1：15(g／mL)ThecontentoftransresveratrolWas1

12、33299／gComparedtheresultswefoundthatultrasonicextractionWaSpreferablemethodbecauseitledtohigheryieldratio，lesssolvent，andshorterextractiontimePurificationtechnologywasstudiedbyusingthecombinativemethodofultrafiltrationan